Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Phenolic Contents of Different Parts of Rhus verniciflua Stokes according to Extraction Conditions

추출조건에 따른 옻나무 부위별 Phenolics 함량

  • Park, Hye Jin (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Sang Hoon (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Li, Meishan (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Min Young (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Sung Tae (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Ji Hyun (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Yoon, Gun Mook (Institute of Health & Environment Research) ;
  • Lee, Junsoo (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jeong, Heon Sang (Department of Food Science and Biotechnology, Chungbuk National University)
  • 박혜진 (충북대학교 식품생명공학과) ;
  • 이상훈 (충북대학교 식품생명공학과) ;
  • 장귀영 (충북대학교 식품생명공학과) ;
  • ;
  • 김민영 (충북대학교 식품생명공학과) ;
  • 김성태 (충북대학교 식품생명공학과) ;
  • 이지현 (충북대학교 식품생명공학과) ;
  • 윤건묵 (충북보건환경연구원) ;
  • 이준수 (충북대학교 식품생명공학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Received : 2014.09.19
  • Accepted : 2014.10.27
  • Published : 2015.01.31
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Abstract

This study investigated changes in phenolic contents of different parts of Rhus verniciflua Stokes (RV) according to extraction conditions. Bark and xylem parts of RV were extracted at 80, 100, 120, 140, and $160^{\circ}C$ for 1, 3, and 5 h, respectively. Major phenolic compounds (gallic acid, protocatechuic acid, fustin, fisetin, sulfuretin, and butein) of RV were analyzed. The gallic acid, fisetin, sulfuretin, and butein contents significantly increased as extraction temperature increased. Protocatechuic acid and fustin contents increased as increasing extraction temperature to $120^{\circ}C$ and decreased afterward. The gallic acid, protocatechuic acid, and butein contents of bark were higher than those of xylem extracts. The optimal extraction conditions of gallic acid, protocatechuic acid, fustin, fisetin, sulfuretin, and butein were $160^{\circ}C/3h$ (380.22 mg%), $120^{\circ}C/1h$ (9.25 mg%), $100^{\circ}C/3h$ (206.97 mg%), $140^{\circ}C/5h$ (93.84 mg%), $140^{\circ}C/5h$ (16.07 mg%) and $160^{\circ}C/5h$ (1.49 mg%), respectively. These results suggest that the optimum extraction temperature and time considering RV extraction yield and cost are $140^{\circ}C$ and 3 h, respectively.

추출 온도와 시간에 따른 옻나무의 주요 페놀화합물의 함량을 부위별로 분석하고 이들의 효율적인 추출조건을 확립하였다. 옻나무 추출물의 gallic acid 함량은 추출 온도가 증가함에 따라 증가하여 수피의 $160^{\circ}C$, 5시간 처리구에서 385.33 mg%로 가장 높았다. Protocatechuic acid 함량은 수피의 $120^{\circ}C$, 1시간 처리구에서 9.25 mg%로 높았지만 그 이상의 온도에서는 크게 감소하였다. Fustin은 $120^{\circ}C$ 이상에서는 감소하였으며 목질의 $100^{\circ}C$, 3시간 처리구에서 206.97 mg%로 가장 높았다. Fisetin은 추출 온도가 증가함에 따라 증가하여 목질부의 $160^{\circ}C$, 5시간 처리구에서 가장 높았다. Sulfuretin은 fisetin과 유사한 경향을 나타내었으며, 목질부의 $160^{\circ}C$, 3시간 처리구에서 16.40 mg%로 가장 높았다. Butein은 추출 온도 및 추출 시간이 증가함에 따라 증가하였으며, 목질부의 $160^{\circ}C$, 5시간 처리구에서 1.49 mg%로 가장 높았다. 통계분석 결과 옻나무의 주요 페놀화합물 추출에 영향을 미치는 요인은 추출 온도가 가장 큰 것으로 나타났다. 유효성분, 추출 수율, 경제성 등을 감안할 때 옻나무 부위별 효율적인 추출조건은 추출 온도 $140^{\circ}C$와 추출 시간 3시간이었다.

Keywords

References

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