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Production of Recombinant GG1234-DsRed Fusion Protein and Its Effect on in vitro CaCO3 Crystallization

재조합 GG1234-DsRed 융합 단백질의 생산 및 In vitro 탄산칼슘 결정화에 미치는 영향에 대한 연구

  • Son, Chaeyeon (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Kim, Jin Ho (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Kim, Ji Ha (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Choi, Yoo Seong (Department of Chemical Engineering and Applied Chemistry, Chungnam National University)
  • 손채연 (충남대학교 응용화학공학과) ;
  • 김진호 (충남대학교 응용화학공학과) ;
  • 김지하 (충남대학교 응용화학공학과) ;
  • 최유성 (충남대학교 응용화학공학과)
  • Received : 2015.10.23
  • Accepted : 2015.11.25
  • Published : 2015.12.27

Abstract

Eggshell-based biocomposites have become attractive due to their exquisite nanostructure and biological properties, which are mainly composed of highly organized calcium carbonate crystals controlled by organic macromolecules such as proteins and polysaccharides. Here, we designed the recombinant fusion protein of a putative eggshell matrix protein named as GG1234 and a fluorescent reporter protein of DsRed. The protein was successfully over-expressed in E. coli and purified by Ni-NTA affinity chromatography. In vitro calcium carbonate crystallization was conducted in the presence of the fusion protein, and morphological change was investigated. The protein inhibited the calcite growth in vitro, and spherical calcium carbonate micro-particles with the diameter of about $20-30{\mu}m$ were obtained. We expect that this study would be helpful for better understanding of eggshell-based biomineralization.

Keywords

References

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