DOI QR코드

DOI QR Code

Correlation of Hanwoo (Korean Native Cattle) Carcass Classification and Oocyte Donor for Blastocyst Production In Vitro

한우 육질등급이 난포란의 배반포 체외생산에 미치는 영향

  • Kim, Kang-Sig (Dept. of Biotechnology, College of Engineering, Daegu University) ;
  • Lee, Hong-Chul (Dept. of Biotechnology, College of Engineering, Daegu University) ;
  • Park, Yong-Su (Kyongbuk Livestock Research Institute) ;
  • Kim, So-Sub (Gwanggaeto Hanwoo Agricultural Company Corporation. Inc.) ;
  • Park, Hum-Dai (Dept. of Biotechnology, College of Engineering, Daegu University)
  • Received : 2015.05.24
  • Accepted : 2015.08.27
  • Published : 2015.09.30

Abstract

These studies were conducted to establish the practical Hanwoo (Korean native cattle) improvement system through the combining of embryo transfer technology and confirming individual Hanwoo oocyte culture system and to investigate that correlation of Hanwoo carcass classification (intramuscular marbling) and oocyte donor for blastocyst production in vitro. In case of Hanwoo, the carcass meat quality grades were divided to grade $1^{{+}{+}}$, $1^{+}$, 1, 2, and 3 depends on fat distribution of longest muscle cross-sectional surface. As results, the numbers of follicular oocytes collected from individual fundamentally-registered Hanwoo yielded $1^{{+}{+}}$, $1^{+}$, 1, 2 and 3 meat quality were 9.5, 9.4, 8.5, 8.8 and 8.8 per ovary, respectively. The numbers of retrieval oocyte from follicles were significantly higher in the cattle yield $1^{{+}{+}}$, $1^{+}$ meat quality than in the cattle yield 1, 2 and 3 meat quality (p<0.05). The rates of blastocyst formation were 18.2, 21.3, 29.4, 30.9, and 31.5% in the cattle yield $1^{{+}{+}}$, $1^{+}$, 1, 2 and 3 meat quality of after in vitro maturation, respectively. It was significantly lower in the cattle yield $^{{+}{+}}$ and $1^{+}$ meat quality than in the cattle yield 1, 2 and 3 meat quality (p<0.05). In order to evaluate embryos quality, TUNNEL assay was conducted for each meat quality grade using blastocyst stage embryo on day 8. The results showed that apoptosis cell number was higher tendency in the cattle yield $1^{{+}{+}}$and $1^{+}$ meat quality (81 and 79, respectively) than in the cattle yield 1, 2 and 3 meat quality (51, 48 and 50, respectively) but there was no statistical significance in each group. After embryo transfer, the conception rate of recipient was 53.5 (23 out of 43), 52.1 (38 out of 73) and 58.0% (58 out of 100) in the meat quality of 1, $1^{+}$ and $1^{{+}{+}}$, respectively. These results showed that the conception rate was significantly higher in the cattle yield 1 meat quality than in the cattle yield $1^{{+}{+}}$, $1^{+}$, 2, and 3 meat quality (p<0.05). In summary, these results indicate that the application of confirming Hanwoo individual oocyte culture system and embryo transfer technology can make good use of the genetic resources conservation and improvement of Hanwoo. Relevance of the meat quality grade and reproductive ability of carcasses of Hanwoo will be considered to be one of the effective means for the associated research with obesity and reproduction.

Keywords

References

  1. Aardema H, Vos PL, Lolicato F, Roelen BA, Knijn HM, Vaandrager AB, Helms JB and Gadella BM. 2011. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol. Reprod. 85:62-69. https://doi.org/10.1095/biolreprod.110.088815
  2. Blondin P and Sirard MA. 1995. Oocytes and follocular morphology as determining characteristics for developmental competence in bovine oocytes. Mol. Reprod. Dev. 41:54-62. https://doi.org/10.1002/mrd.1080410109
  3. Boelhauve M, Sinowatz F, Wolf E and Paula-Lopes FF. 2005 Maturation of bovine oocytes in the presence of leptin improves development and reduces apoptosis of in vitroproduced blastocysts. Biol. Reprod. 73:737-44. https://doi.org/10.1095/biolreprod.105.041103
  4. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF and Dressel MA. 1982. Normal development following in vitro fertilization in the cow. Biol. Reprod. 27:147-158. https://doi.org/10.1095/biolreprod27.1.147
  5. Carolan C, Lonergan P, Khatir H and Mermillod P. 1996. In vitro production of bovine embryos using individual oocytes. Mol. Reprd. Dev. 41:145-150.
  6. Cerri RL, Juchem SO, Chebel RC, Rutigliano HM, Bruno RG and Galvao KN. 2009. Effect of fat source differing in fatty acid profile on metabolic parameters, fertilization, and embryo quality in highproducing dairy cows. J. Dairy Sci. 92:1520-31. https://doi.org/10.3168/jds.2008-1614
  7. Chi MM, Schlein AL and Moley KH. 2000. High insulin-like growth factor 1 (IGF-1) and insulin concentrations trigger apoptosis in the mouse blastocyst via down-regulation of the IGF-1 receptor. Endocrinology. 141:4784-4792. https://doi.org/10.1210/endo.141.12.7816
  8. Fabian D, Koppel J and Maddox-Hyttel P. 2005. Apoptotic processes during mammalian preimplantation development. Theriogenology. 64:221-231. https://doi.org/10.1016/j.theriogenology.2004.11.022
  9. Farley D, Tejero ME, Comuzzie AG, Cox L and Werner SL. 2009. Feto-placental adaptations to maternal obesity in the baboon. Placenta. 30:752-760. https://doi.org/10.1016/j.placenta.2009.06.007
  10. Fedorscak P, Dale PO, Storeng R, Ertzeid G, Bjercke S and Oldereid N. 2004. Impact of overweight and underweight on assisted reproduction treatment. Hum. Reprod. 19:2523-2528. https://doi.org/10.1093/humrep/deh485
  11. Igosheva N, Abramov AY, Poston L, Eckert JJ, Fleming TP and Duchen MR. 2010. Maternal diet-induced obesity alters mitochondrial activity and redox status in mouse oocytes and zygotes. PLoS. One. 5:e10074. https://doi.org/10.1371/journal.pone.0010074
  12. Itagaki Y, Sato S and Shitanaka Y. 1993. Sexing of bovine embryo with male-specific repetitive DNA by polymerase chain reaction: Sexing of bovine embryo and production of calves with predicted sex. J. Reprod. Dev. 39:65-72. https://doi.org/10.1262/jrd.39.65
  13. Sun J, Xu Y, Deng H, Sun S, Dai Z and Sun Y. 2010. Intermittent high glucose exacerbates the aberrant production of adiponectin and resistin through mitochondrial superoxide overproduction in adipocytes. Journal of Molecular Endocrinology. 44:179-185. https://doi.org/10.1677/JME-09-0088
  14. Jungheim ES, Schoeller EL, Marquard KL, Louden ED, Schaffer JE and Moley KH. 2010. Diet-induced obesity model: Abnormal oocytes and persistent growth abnormalities in the offspring. Endocrinology. 151:4039-4046. https://doi.org/10.1210/en.2010-0098
  15. Kajihara Y, Kometani N, Kobayashi S, Shitanaka Y, Koshiba Y, Shiraiwa K and Goto K. 1992. Pregnancy rates and births after co-culture of cumulus cells with bovine embryos derived from in vitro fertilization of in vitro matured follicular oocytes. Theriogenology. 3:264.
  16. Kredel LI and Siegmund B. 2014. Adipose-tissue and intestinal inflammation-visceral obesity and creeping fat. Front. Immunol. 24;5:462.
  17. Kubandova J, Cikos S, Burkus J, Czikkova S, Koppel J and Fabian D. 2014. Amount of maternal body fat significantly affected the quality of isolated mouse preimplantation embryos and slowed down their development. Theriogenology. 15;81:187-195.
  18. Lashen H, Ledger W, Bernal AL and Barlow D. 1999. Extremes of body mass do not adversely affect the outcome of superovulation and in vitro fertilization. Hum. Reprod. 14:712-715. https://doi.org/10.1093/humrep/14.3.712
  19. Leroy JL, Vanholder T, Delanghe JR, Opsomer G, Van Soom A, Bols PE and de Kruif A. 2004. Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows. Anim. Reprod. Sci. 80:201-211. https://doi.org/10.1016/S0378-4320(03)00173-8
  20. Leroy JL, Vanholder T, Delanghe JR, Opsomer G, Van Soom A, Bols PE, Dewulf J and de Kruif A. 2004. Metabolic changes in follicular fluid of the dominant follicle in highyielding dairy cows early post partum. Theriogenology. 15; 62:1131-1143.
  21. Leroy JL, Vanholder T, Mateusen B, Christophe A, Opsomer G, de Kruif A, Genicot G and Van Soom A. 2005. Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction. 130:485-95. https://doi.org/10.1530/rep.1.00735
  22. Lewis CG, Warnes GM, Wang X and Matthews CD. 1990. Failure of body mass index or body weight to influence markedly the response to ovarian stimulation in normal cycling women. Fertil. Steril. 53:1097-1099. https://doi.org/10.1016/S0015-0282(16)53594-8
  23. Luzzo KM, Wang Q, Purcell SH, Chi M, Jimenez PT and Grindler N. 2012. High fat diet induced developmental defects in the mouse: Oocyte meiotic aneuploidy and fetal growth retardation/brain defects. PLoS. One. 7:e49217. https://doi.org/10.1371/journal.pone.0049217
  24. Mermillod P, Wils C, Massip A and Dessy F. 1992. Collection of oocytes and production of blastocysts in vitro from individual, slaughtered cows. J. Reprod. Fert. 96:717-723. https://doi.org/10.1530/jrf.0.0960717
  25. Minge CE, Bennett BD, Norman RJ and Robker R. 2008. Peroxisome proliferator-activated receptor-gamma agonist rosiglitazone reverses the adverse effects df diet-induced obesity on oocyte quality. Endocrionology. 149:2646-2656. https://doi.org/10.1210/en.2007-1570
  26. Moley KH, Chi MM and Mueckler MM. 1998. Maternal hyperglycemia alters glucose transport and utilization in mouse preimplantation embryos. Am. J. Physiol. 275:38-47.
  27. Ponter AA, Parsy AE, Saade M, Mialot JP, Ficheux C and Duvaux-Ponter C. 2006. Effect of a supplement rich in linolenic acid added to the diet of post partum dairy cows on ovarian follicle growth, and milk and plasma fatty acid compositions. Reprod. Nutr. Dev. 46:19-29. https://doi.org/10.1051/rnd:2005058
  28. Rosenkrans CF Jr, Zeng GQ, MCNamara GT, Schoff PK and First NL. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod. 49:459-462. https://doi.org/10.1095/biolreprod49.3.459
  29. Shea BF. 1999. Determining the sex of bovine embryo using polymerase chain reaction results: A six-year retrospective study. Theriogenology. 51:841-854. https://doi.org/10.1016/S0093-691X(99)00030-8
  30. Styne-Gross A, Elkind-Hirsch K and Scott RT. 2005. Obesity does not impact implantation rates or pregnancy outcome in women attempting conception through oocyte donation. Fertil. Steril. 83:1629-1634. https://doi.org/10.1016/j.fertnstert.2005.01.099
  31. Tersigni C, Di Nicuolo F, D'Ippolito S, Veglia M, Castellucci M and Di Simone N. 2011. Adipokines: New emerging roles in fertility and reproduction. Obstet. Gynecol. Surv. 66:47-63. https://doi.org/10.1097/OGX.0b013e318217b0a4
  32. Thangavelu G, Colazo MG, Ambrose DJ, Oba M, Okine EK and Dyck MK. 2007. Diets enriched in unsaturated fatty acids enhance early embryonic development in lactating Holstein cows. Theriogenology. 68:949-957. https://doi.org/10.1016/j.theriogenology.2007.07.002
  33. Thibier M. 2001. Identified and unidentified challenges for reproductive biotechnologies regarding infectious diseases in animal and public health. Theriogenology. 56:1465-1481. https://doi.org/10.1016/S0093-691X(01)00647-1
  34. Wakefield SL, Lane M, Schulz SJ, Hebart ML and Thompson JG. 2008. Maternal supply of omega-3 polyunsaturated fatty acids alter mechanism involved in oocyte and early embryo development in the mouse. Am. J. Physiol. Endocrinol. Metab. 294:425-434. https://doi.org/10.1152/ajpendo.00409.2007
  35. Wathes DC, Abayasekara DR and Aitken RJ. 2007. Polyunsaturated fatty acids in male and female reproduction. Biol Reprod. 77:190-201. https://doi.org/10.1095/biolreprod.107.060558
  36. Wiemer KE, Watson AJ, Polanski V, Mckenna AI, Fick GH and Shultz GA. 1991. Effects of maturation and co-culture treatments on the developmental capacity of early bovine embryos. Mol. Reprod. Dev. 30:330-338. https://doi.org/10.1002/mrd.1080300407
  37. Wittemer C, Oh J, Bailly M, Bettahar-Lebugle K and Nisand I. 2000. Does body mass index of fertile women have an impact on IVF procedure outcome J. Assist. Reprod. Genet. 17:547-552. https://doi.org/10.1023/A:1026477628723
  38. Wu B and Zan L. (2012). Enhance beef cattle improvement by embryo biotechnologies. Reprod. Domest. Anim. 47:865-871. https://doi.org/10.1111/j.1439-0531.2011.01945.x
  39. Zullo F, Di Carlo C, Pellicano M, Catizone C, Mastrantonio P and Nappi C. 1996. Superovulation with urinary human follicle- stimulating hormone: Correlations with body mass index and body fat distribution. Gynecol. Endocrinol. 10:17-21.
  40. Utsumi K, Hayashi M and Takakura R. 1993. Embryo sex selection by a rat male-specific antibody and the cytogenetic and developmental confirmation in cattle embryo. Mol. Reprod. Dev. 34:25-32. https://doi.org/10.1002/mrd.1080340105
  41. 고광두, 정길생, 이기만. 1981. 한우의 수정란이식에 관한 연구 III. 수정란의 비외과적 채취와 이식. 한국축산학회지. 23: 331-337.
  42. 김용준, 정구남, 이해이, 조성우, 김용수, 유일정. 2000. 한우체외수정란 Biopsy 후 PCR 기법을 이용한 성판정과 성감별 수정란의 이식. 한국수정란이식학회지. 15:219-230.
  43. 김영훈, 고진철, 오창언, 강승률, 강보석, 오성종, 김창능, 송중용, 김일화. 2006. CIDR를 이용한 제주한우 및 흑우의 체내 수정란 생산과 이식. 한국수정란이식학회지. 21;191-198.
  44. 박성재, 양보석, 임기순, 성환후, 양병철, 장원경, 정일정, 정기화, 심보웅, 박충생. 2000. 한우에 있어서 초음파기기를 이용한 생체내 개체별 난자 채취 빈도 및 수정란 생산 효율에 관한 연구. 한국수정란이식학회지. 15:1-8.
  45. 박용수, 김소섭, 박흠대, 박현정, 김재명. 2005. 한우 체외수정란이 이식된 수란우의 임신과 유산에 영향을 미치는 수정란측 요인. 한국수정란이식학회지. 20:89-95.
  46. 박희성, 이지삼, 진종인, 박준규, 홍승표, 이명열, 정장용. 2001. 유전자 분석을 통하여 선발된 한우로부터 초음파 유래 체외수정란 이식에 의한 고품질 한우 생산기술의 실용화 II. DNA 검정우로부터 초음파 유래 체외수정란의 생산에 관한 연구. 한국수정란이식학회지. 16:193-201.
  47. 조성근, 노국진, 이정국, 이효종, 최상용, 박충생. 1992. 체외배양 조건이 소 체외 수정란의 생산에 미치는 효과. 한국수정란이식학회지. 15:271-277.
  48. 황환섭, 장현용, 김성곤, 김종택, 박춘근, 정희태, 김정익, 양부근. 2004. 한우 체외성숙-체외 수정란의 수정란 이식에 관한 연구. 한국수정란이식학회지. 19:1-10.