The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Melon necrotic spot virus Occurring on Watermelon in Korea

  • Kwak, Hae-Ryun (Department of Crop Protection, National Academy of Agricultural Science, RDA) ;
  • Kim, Jeong-Soo (Department of Plant Medicine, Andong National University) ;
  • Cho, Jeom-Deog (Department of Plant Medicine, Andong National University) ;
  • Lee, Joong-Hwan (Institute for Bioresources Research, Gyeongsangbukdo Agricultural Research and Extension Service, KBARES) ;
  • Kim, Tae-sung (Gyeongsangnamdo Agricultural Research and Extension Service) ;
  • Kim, Mi-Kyeong (Department of Crop Protection, National Academy of Agricultural Science, RDA) ;
  • Choi, Hong-Soo (Department of Crop Protection, National Academy of Agricultural Science, RDA)
  • Received : 2014.11.27
  • Accepted : 2015.08.11
  • Published : 2015.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Melon necrotic spot virus (MNSV) was recently identified on watermelon (Citrullus vulgaris) in Korea, displaying as large necrotic spots and vein necrosis on the leaves and stems. The average occurrence of MNSV on watermelon was found to be 30-65% in Hapcheon and Andong City, respectively. Four isolates of the virus (MNSV-HW, MNSV-AW, MNSV-YW, and MNSV-SW) obtained from watermelon plants in different areas were non-pathogenic on ten general indicator plants, including Chenopodium quinoa, while they infected systemically six varieties of Cucurbitaceae. The virus particles purified by 10-40% sucrose density gradient centrifugation had a typical ultraviolet spectrum, with a minimum at 245 nm and a maximum at 260 nm. The morphology of the virus was spherical with a diameter of 28-30 nm. Virus particles were observed scattered throughout the cytoplasm of watermelon cells, but no crystals were detected. An ELISA was conducted using antiserum against MNSV-HW; the optimum concentrations of IgG and conjugated IgG for the assay were $1{\mu}l/ml$ and a 1:8,000-1:10,000 dilutions, respectively. Antiserum against MNSV-HW could capture specifically both MNSV-MN from melon and MNSV-HW from watermelon by IC/RT-PCR, and they were effectively detected with the same specific primer to produce product of 1,172 bp. The dsRNA of MNSV-HW had the same profile (4.5, 1.8, and 1.6 kb) as that of MNSV-MN from melon. The nucleotide sequence of the coat protein of MNSV-HW gave a different phylogenetic tree, having 17.2% difference in nucleotide sequence compared with MNSV isolates from melon.

Keywords

References

  1. Avgelis, A. D. 1989. Watermelon necrosis caused by a strain of melon necrotic spot virus. Plant Pathol. 38:618-622. https://doi.org/10.1111/j.1365-3059.1989.tb01461.x
  2. Bos, L., Van Dorst, H. J. M., Huttinga, H. and Maat, D. Z. 1984. Further characterization of Melon necrotic spot virus causing severe disease in glasshouse cucumbers in the Netherlands and its control. Neth. J. Plant Pathol. 90:55-69. https://doi.org/10.1007/BF01999953
  3. Cho, J. D., Kim, J. S., Kim, H. R., Chung, B. N. and Ryu, K. H. 2006. Convenient nucleic acid detection for Tomato spotted wilt virus: Virion captured/RT-PCR (VC/RT-PCR). Res. Plant Dis. 12:139-143. https://doi.org/10.5423/RPD.2006.12.2.139
  4. Choi, G. S., Kim, J. H. and Kim, J. S. 2003. Characterization of Melon necrotic spot virus isolated from muskmelon. Plant Pathol. J. 19:123-127. https://doi.org/10.5423/PPJ.2003.19.2.123
  5. Gonzalez-Garza, R., Gumpz, D. J., Kishaba, A. N. and Bohn, G. W. 1979. Identification, seed transmission and host range pathogenicity of a California isolate of Melon necrotic spot virus. Phytopathology 69:340-345. https://doi.org/10.1094/Phyto-69-340
  6. Herrera, J. A., Cebrian, M. C. and Jorda, C. 2006. First report of Melon necrotic spot virus in Panama. Plant disease 90:1261
  7. Herrera, J. A., Cordoba, M. C., Cebrian, M. C., Rosselo, J. A., Alfaro, A. and Jorda, C. 2010. Genetic diversity of Melon necrotic spot virus and Olpidium isolates from different origins. Plant Pathol. 59:240-251. https://doi.org/10.1111/j.1365-3059.2009.02208.x
  8. Hibi, T. 1986. Melon necrotic spot virus. CMI/AAB Description of plant viruses, No. 302.
  9. Huitron, M. V., Ricardez, M. and Camacho F. 2009. Influence of grafted watermelon plant density on yield and quality in soil infested with Melon necrotic spot virus. HortScience 44:1838-1841.
  10. Kim, J. S., Lee, S. H., Choi, H. S., Choi, G. S., Cho, J. D. and Chung, B. N. 2008. Survey of viral diseases occurrence on major crops in 2007. Res. Plant Dis. 14:1-9. https://doi.org/10.5423/RPD.2008.14.1.001
  11. Kishi, K. 1966. Necrotic spot of melon, a new virus disease. Ann. Phytopath. Soc. Japan 57:558-567.
  12. Mallor, C., Alvarez, M. and Luis-Arteaga, M. 2003. A resistance to systemic symptom expression of Melon necrotic spot virus in melon. J. Amer. Soc. Hort. Sci. 128:541-547.
  13. Matsuo, K., Kameya-Iwaki, M. and Ota, T. 1991. Two new strains of Melon necrotic spot virus. Ann. Phytopath. Soc. Japan 57:558-567. https://doi.org/10.3186/jjphytopath.57.558
  14. Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis of double- stranded RNA from virus infected plant and fungal tissue. Phytopathology 69:854-858. https://doi.org/10.1094/Phyto-69-854
  15. Ohki, T., Sako, I., Kanda, A., Mochizuki, T., Honda, Y. and Tsuda, S. 2008. A new strain of Melon necrotic spot virus that is unable to systemically infect Cucumis melo. Phytopathology 98:1165-1170. https://doi.org/10.1094/PHYTO-98-11-1165
  16. Park, J. W., Jang, T. H., Song, S. H., Choi, H. S. and Ko, S. J. (2010). Studies on the Soil Transmission of CGMMV and Its Control with Crop Rotation. Kor. J. Pestic. Sci. 14:473-477.
  17. Riviere, C. J., Pot, J., Tremaine, J. H. and Rochon, D. M. 1989. Coat protein of Melon necrotic spot carmovirus is more similar to those of Tombusviruses than those of Carmoviruses. J. Gen. Virol. 70:3033-3042. https://doi.org/10.1099/0022-1317-70-11-3033
  18. Tomlinson, J. A. and Thomas, B. J. 1986. Studies on Melon necrotic spot virus disease of cucumber and on the control of the fungus vector (Olpidium radicale). Ann. Appl. Biol. 108:71-80. https://doi.org/10.1111/j.1744-7348.1986.tb01967.x