Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Probiotic Properties of Lactic Acid Bacteria Isolated from Commercial Raw Makgeolli

시판 생막걸리에서 분리한 유산균의 프로바이오틱스 기능성 연구

  • Jung, Sang-Eun (Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Sae-Hun (Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University)
  • 정상은 (고려대학교 생명과학대학 식품공학부) ;
  • 김세헌 (고려대학교 생명과학대학 식품공학부)
  • Received : 2014.07.10
  • Accepted : 2014.11.26
  • Published : 2015.02.28
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Abstract

The purpose of this study was to characterize the lactic acid bacteria found in makgeolli in terms of bacterial identity and gastric compatibility. Lactic acid bacteria were isolated from commercial raw makgeolli and separated into six strains that are resistant to gastric acidity and bile acid. These strains were identified by analysis of their 16S rDNA, as Lactobacillus plantarum BSM-2 and EHJ-1, Lactobacillus casei GSM-3 and EHJ-2, Lactobacillus brevis BSM-3 and Pediococcus pentosaceus TJH-1. All strains exhibited adhesion to intestines, showing that they were probiotic. We also found that L. plantarum BSM-2 had excellent resistance to bile acid as well as antioxidant activity. Taken together with its antibacterial properties and ability to lower cholesterol, our data suggest that L. plantarum BSM-2 was the most beneficial probiotic among the six strains.

본 연구에서는 시판 생막걸리에서 분리한 유산균의 probiotics 특성을 연구하기 위해 내산성, 내담즙성, 장 부착능에 효능이 있는 6개 균주를 선별하여 동정하였다. 생막걸리에서 분리한 유산균을 pH 2.5의 산성 및 담즙산(0.3% oxgall) 조건에서 내산성과 내담즙성을 측정한 결과 BSM-2, BSM-3, GSM-3, TJH-1, EHJ-1, EHJ-2의 6개 균주에서 각각 $10^7$, $10^8CFU/mL$ 높은 생존률을 나타내어 우수한 내산성과 내담즙성을 확인할 수 있었다. 내산성과 내담즙성에 효능이 있는 6개 균주의 16S rDNA 분석 결과, L. plantarum (BSM-2, EHJ-1) L. casei (GSM-3, EHJ-2), L. brevis (BSM-3), P. pentosaceus (TJH-1)의 4개 group이 동정되었다. 장부착능에서는 다른 유산균에 비해 장내 부착능이 뛰어난 것으로 알려진 L. rhamnosus GG를 대조군으로 사용하였으며 HT-29 cell에 대해 6종의 유산균이 모두 장 부착능을 보였으며 L. casei(GSM-3, EHJ-2)가 가장 장 부착능이 높았다. 항산화능력은 L. plantarum BSM-2와 EHJ-1에서 각각 32.44%, 24.65%의 DPPH radical 소거능을 보였다. 시판 생막걸리에서 분리된 유산균은 다양한 식중독 원인 세균에 대하여 항균활성을 지니고 있었으며, 본 연구에서는 유산균이 생성하는 항균물질인 bacteriocin이 아닌 유산균 발효 중 젖산을 생성하면서 pH의 저하로 항균활성을 보인다는 결과를 확인할 수 있었다. Probiotics로의 기능성을 가진 6개 균주의 콜레스테롤 저하능력은 L. plantarum BSM-2와 EHJ-1에서 각각 68.82%, 56.38%의 높은 감소율을 나타내었다. 시판 생막걸리에서 분리한 유산균의 probiotic 특성을 조사해 본 결과 L. plantarum BSM-2, EHJ-1, L. brevis BSM-3, L. casei GSM-3, EHJ-2, P. pentosaceus TJH-1 모두 프로바이오틱스의 기본특성을 지니고 있어 프로바이오틱 균주로서 이용가치가 충분하다고 판단되어지며, L. plantarum BSM-2가 내산성, 내담즙성이 가장 우수한 결과를 나타내었고, 장 부착성이 우수하였으며, 뛰어난 항산화 능력과 항균력을 지니며, 콜레스테롤 저하능도 뛰어나 가장 우수한 프로바이오틱 균주라고 판단된다.

Keywords

References

  1. Lee JS, Lee TS, Park SO, Noh BS. Flavor components in mash of takju prepared by different raw materials. Korean J. Food Sci. Technol. 28: 316-323 (1996)
  2. Lee TS, Han EH. Volatile flavor components in mash of takju prepared by using Aspergillus oryzae nuruks. Korean J. Food Sci. Technol. 33: 366-372 (2001)
  3. Kwon YH, Lee AR, Kim JH, Kim HR, Ahn BH. Changes of physicochemical properties and microbial during storage of commercial makgeolli. Korean J. Mycol. 40: 210-214 (2012) https://doi.org/10.4489/KJM.2012.40.4.210
  4. Seo DH, Jung JH, Kim HY, Kim YR, Ha SJ, Kim YC, Park CS. Identification of lactic acid bacteria involved in traditional Korean rice wine fermentation. Food Sci. Biotechnol. 16: 994-998 (2007)
  5. Jin J, Kim SY, Jin Q, Eom HJ, Han NS. Diversity analysis of lactic acid bacteria in takju, Korean rice wine. J. Microbiol. Biotechn. 18: 1678-1682 (2008)
  6. Kim YH, Min JH, Kang MG, Kim JH, Ahn BH, Kim HG, Lee JS. Physicochemical properties, lactic acid bacteria content and physiological functionalities of Korean commercial makgeolli. Korean J. Microbiol. Biotechnol. 40: 325-332 (2012) https://doi.org/10.4014/kjmb.1207.07006
  7. Jo KY, Ha DM. Isolation and identification of the lactic acid bacteria from nuruk. J. Korean Soc. Appl. Biol. Chem. 38: 95-99 (1995)
  8. Lee JH, Yu TS. Identification and characteristics of lactic acid bacteria isolated from nuruk. Korean J. Biotechnol. Bioeng. 15: 359-365 (2000)
  9. Hoon SS, Lee CH, Lee SH, Park JM, Lee HJ, Bai DH, Yoon SS, Choi JB, Park Y S. Analysis of microflora profile in Korean traditional nuruk. J. Microbiol. Biotechnol. 23: 40-46 (2013) https://doi.org/10.4014/jmb.1210.10001
  10. Kim SY, Yoo KS, Kim JE, Kim JS, Jung JE, Jin Q, Eom HJ, Han NS. Diversity analysis of lactic acid bacteria in takju, Korean rice wines, by PCR-denaturing gradient gel electrophoresis. Korean J. Food Sci. Technol. 19: 749-755 (2010) https://doi.org/10.1007/s10068-010-0105-z
  11. Kim HR, Lee AR, Kim JH, Ahn BH. Microbial dynamics of commercial makgeolli depending on the storage temperature. J. Microbiol. Biotechnol. 22: 1101-1106 (2012) https://doi.org/10.4014/jmb.1112.12063
  12. Lee HR. Diversity of lactic acid bacteria (LAB) in Korean draft rice wines and studies on the functional materials in selected LABs. MS thesis, Kyung Hee University, Seoul, Korea (2012)
  13. Yoo KS. Lactic acid bacterial diversity in makgeolli and its effects on fermentation process and quality. PhD thesis, Chungbuk National University, Daejeon, Korea (2013)
  14. Lin MYN, Chang FJ. Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC15708 and lactobacillus acidophilus ATCC 4356. Digest. Dis. Sci. 45: 1617-1622 (2000) https://doi.org/10.1023/A:1005577330695
  15. Gilliland SE, Nelson CR, Maxwell C. Assimilation of cholesterol by lactobacillus acidophilus. Appl. Environ. Microbiol. 49: 377 (1985)
  16. Rudel LL, Morris MD. Determination of cholesterol using o-phtalaldehyde. J. Lipid Res. 14: 364-366 (1973)
  17. Sim JH, Oh SJ, Kim SK, Baek YJ. Comparative tests on the acid tolerance of some lactic acid bacteria species isolated from lactic fermented products. Korean J. Food Sci. Technol. 27: 101-104 (1995)
  18. Saarela M, Mogensen G, Fondn R, Mtt J, Mattila-Sandholm T. Probiotic bacteria: safety, functional and technological properties. J. Biotechnol. 84: 197-215 (2000) https://doi.org/10.1016/S0168-1656(00)00375-8
  19. Gilliland SE. Beneficial interrelationships between certain microorganisms and humans: Candidate microorganisms for use as dietary adjuncts. J. Food Prot. 42: 164-167 (1979)
  20. McDonald LC, Fleming HP. Acid tolerance of Leuconostoc mesenteriodes and Lactobacillus plantarum. Appl. Environ. Microbiol. 56: 2120-2124 (1990)
  21. Jin LZ, Ho YW, Abdullah N, Jalaludin S. Acid and bile tolerance of Lactobacillus isolated from chicken intestine. Lett. Appl. Microbiol. 27: 183-185 (1998) https://doi.org/10.1046/j.1472-765X.1998.00405.x
  22. Maria GK, Evdokia KM, Ioannis GO, Adamantini AK. In vitro assessment of probiotic properties of Lactobacillus strains from infant gut microflora. Food Biotechnol. 22: 1-17 (2008) https://doi.org/10.1080/08905430701707844
  23. Vidhyasagar V, Jeevaratnam K. Evaluation of Pediococcus pentosaceus strains isolated from idly batter for probiotic properties in vitro original research article. J. Funct. Food. 5: 235-243 (2013) https://doi.org/10.1016/j.jff.2012.10.012
  24. Klaenhmmer TR, Kleeman EG. Growth characteristics bile sensitive and freeze damage in colonial variants of Lactobacillus acidophilus. Appl. Environ. Microbiol. 41: 1461-1667 (1981)
  25. Dunne CL, O'Mahoney L, Murphy L, Thornton D, Morrissey D, O'Halloran S, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, O'Sullivan GC, Shanahan F, Collins JK. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386S-392S (2001)
  26. Gronlund MM, Isoaurk E, Kirjavainen PV, Ouwehand AC, Salminen SJ. Adhesion of probiotic micro-organism to intestinal mucus. Int. Dairy J. 9: 623-630 (1999) https://doi.org/10.1016/S0958-6946(99)00132-6
  27. Kim SE. Identification of lactic acid bacteria (LAB) from mukeunji, a long-term ripened kimchi and analysis of availability of LAB as probiotics. MS thesis, Kyung Hee University, Seoul, Korea (2009)
  28. Aguirre M, Collins MD. Lactic acid bacteria and human clinical infection. J. Appl. Bacteriol. 75: 95-107 (1993) https://doi.org/10.1111/j.1365-2672.1993.tb02753.x
  29. Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochemie 70 : 337-349 (1988) https://doi.org/10.1016/0300-9084(88)90206-4
  30. Lim SM, Im DS, Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J. Microbiol. Biotechnol. 19: 178-186 (2008)
  31. Song YJ, Park SH, You JY, Cho YS, Oh KH. Antibacterial activity against food-poisoning causing bacteria and characterization of Lactobacillus plantarum YK-9 isolated from kimchi. Korean J. Biotechnol. Bioeng. 24: 273-278 (2009)
  32. Baek H, Choi MS, Oh KH. Characterization and antibacterial activity of Lactobacillus casei HK-9 Isolated from Korean rice wine, makgeolli. Korean J. Biotechnol. Bioeng. 27: 161-166 (2012) https://doi.org/10.7841/ksbbj.2012.27.3.161
  33. Ahn DK, Han TW, Shin HY, Jin IN, Ghim SY. Diversity and antibacterial activity of lactic acid bacteria isolated from kimchi. J. Korean Microbiol. Biotechnol. 2: 191-196 (2003)
  34. Kim MJ, Kim GR. In vitro evaluation of cholesterol reduction by lactic acid bacteria extracted from kimchi. Korean J. Culin. Res. 12: 259-268 (2006)
  35. Nguyen TD, Kang JH, Lee MS. Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterollowering effects. Int. J. Food Microbiol. 113: 358-361 (2007) https://doi.org/10.1016/j.ijfoodmicro.2006.08.015
  36. Wang Y, Xu N, Xi A, Ahmed Z, Zhang B, Bai X. Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl. Microbiol. Biotechnol. 84: 341-347 (2009) https://doi.org/10.1007/s00253-009-2012-x
  37. Tsai Ty, Chu LH, Lee CL, Pan TM. Atherosclerosis-preventing activity of lactic acid bacteria-fermneted milk-soymilk supplemented with momordica charantia. J. Agr. Food Chem. 57: 2065- 2071 (2009) https://doi.org/10.1021/jf802936c
  38. Bang JH, Shin HJ, Choi HJ, Kim DW, Ahn CS, Jeong YK, Joo WH. Probiotic potential of Lactobacillus isolates. J. Life Sci. 22: 251-258 (2012) https://doi.org/10.5352/JLS.2012.22.2.251

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