Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Radical Scavenging and Anti-Obesity Effects of 50% Ethanol Extract from Fermented Curcuma longa L.

발효울금 50% 에탄올 추출물의 라디칼 소거능 및 지방형성 억제 효과

  • Kim, Jihye (Division of Food and Nutrition, Chonnam National University) ;
  • Jun, Woojin (Division of Food and Nutrition, Chonnam National University)
  • 김지혜 (전남대학교 식품영양과학부) ;
  • 전우진 (전남대학교 식품영양과학부)
  • Received : 2014.12.16
  • Accepted : 2015.01.07
  • Published : 2015.02.28
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Abstract

In this study, free radical scavenging activities (ABTS, DPPH, NBT, TBARS, and ORAC) and anti-obesity potential were evaluated using 50% ethanol extract from fermented Curcuma longa L. (FCE50). FCE50 showed free radical scavenging activities and anti-oxidant potential. Lipid accumulation and intracellular TG content were significantly reduced by 25.8% and 28.6%, respectively, by $250{\mu}g/mL$ of FCE50 compared to adipocytes. Glucose uptake was significantly reduced by 12.0%. FCE50 significantly reduced mRNA expression of acetyl-CoA carboxylase in 3T3-L1 cells. These results indicate that the anti-adipogenic effect of FCE50 might be due to its radical scavenging activity and anti-oxidant potential.

본 연구에서는 FCE50을 대상으로 다양한 항산화 실험을 실시한 결과, FCE50이 항산화능을 보유하고 있음을 확인하였다. 또한 FCE50은 3T3-L1 전지방세포의 분화과정 중 세포내 지방축적을 유의적으로 감소시키며 포도당 유입을 감소시켰다. 지방합성과 관련된 ACC 효소의 mRNA 발현량을 측정한 결과, 완전히 분화된 지방세포와 비교하여 FCE50의 처리 시 그 발현량이 현저히 감소됨을 확인하였다. 이상의 결과로부터 FCE50은 항산화 활성을 보유하고 있으며, 이는 FCE50이 지방세포로의 분화과정 중 지방형성을 유의적으로 감소시키는 결과에 영향을 미치는 것으로 사료된다.

Keywords

References

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