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Biological activities and acute oral toxicity of citronella and lemongrass oil

Citronella 및 lemongrass oil의 생리활성 및 단회 경구 투여 독성시험

  • 박지용 ((주)크린바이오 부설연구소) ;
  • 김진윤 (경북대학교 수의과대학) ;
  • 장승희 ((주)크린바이오 부설연구소) ;
  • 김해중 ((주)크린바이오 부설연구소) ;
  • 이승진 (경북대학교 수의과대학) ;
  • 박승춘 (경북대학교 수의과대학)
  • Received : 2015.02.09
  • Accepted : 2015.03.05
  • Published : 2015.03.31

Abstract

This study was performed to investigate the antibacterial, antioxidant, and termite repellent effects of citronella oil (CiO) and lemongrass oil (LO). When the antibacterial activity against Staphylococcus (S.) aureus with various levels of antibacterial resistance were tested, a 0.05% concentration of CiO and LO completely inhibited the growth of all tested S. aureus strains. Evaluation of the antioxidant effect demonstrated that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of CiO was 2~3 times greater than that of LO. Among trial products made with various combinations of CiO and LO, a CiO : LO ratio of 6 : 4 had the most potent termite repellent effects. Assessment of acute toxicity of the trial product showed that the $LD_{50}$ was more than 2,000 mg/kg. Based on the above results, CiO and LO have antibacterial, antioxidant, and termite repellent activities. Therefore, both compounds could be potential termites repellent reagents.

Keywords

References

  1. Adukwu EC, Allen SC, Phillips CA. The anti-biofilm activity of lemongrass (Cymbopogon flexuosus) and grapefruit (Citrus paradisi) essential oils against five strains of Staphylococcus aureus. J Appl Microbiol 2012, 113, 1217-1227. https://doi.org/10.1111/j.1365-2672.2012.05418.x
  2. Baik MK, Hwang JM, Jung KS, Kim TW, Kim MC, Lee YJ, Cho YB, Park SW, Lee HS, Ku DS, Jeong JC, Kim KG, Choi DS, Shin EH, Hwang JH, Lee JS, Kim SS, Bae YS. Checklist of Korean Insects. Nature & Ecology, Seoul, 2010.
  3. Bakkali F, Averbeck S. Averbeck D, Idaomar M. Biological effects of essential oils - a review. FoodChem Toxicol 2008, 46, 446-475. https://doi.org/10.1016/j.fct.2007.09.106
  4. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  5. Bruneton J. Pharmacognosy, Phytochemistry, Medicinal Plants. 2nd ed. pp. 461-780, Lavoisier, New York, 1999.
  6. Chandra H, Ah AF. Lipoxygenase inhibitory, antioxidant and antimicrobial activities of selected essential oils. Asian J Pharma Clin Res 2014, 7, 79-83.
  7. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-Eleventh Edition. CLSI document M02-A11. Clinical and Laboratory Standards Institute, Wayne, 2012.
  8. De Martino L, De Feo V, Fratianni F, Nazzaro F. Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. Nat Prod Commun 2009, 4, 741-750.
  9. Desmarchelier JH. Grain protectants: trends and developments. In: Highley E, Wright EJ, Banks HJ, Champ BR (eds.). Stored Product Protection: Proceedings of the 6th International Working Conference on Stored-Product Protection: 17-23 April 1994, Canberra, Australia, Volume 1. pp. 722-728, CAB International, Wallingford, 1994.
  10. Dryden MS, Dailly S, Crouch M. A randomized, controlled trial of tea tree topical preparations versus a standard topical regimen for clearance of MRSA colonization. J Hosp Infect 2004, 56, 283-286. https://doi.org/10.1016/j.jhin.2004.01.008
  11. Fandohan P, Gnonlonfin B, Laleye A, Gbenou JD, Darboux R, Moudachirou M. Toxicity and gastric tolerance of essential oils from Cymbopogon citratus, Ocimum gratissimum and Ocimum basilicum in Wistar rats. Food Chem Toxicol 2008, 46, 2493-2497. https://doi.org/10.1016/j.fct.2008.04.006
  12. Han SH, Lee KS, Chung YJ. Characteristic of termite inhibits in South Korea and the control. Conserv Stud 1998, 19, 133-158.
  13. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 1999, 86, 985-990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  14. Kim JK, Kang CS, Lee JK, Kim YR, Han HY, Yun HK. Evaluation of repellency effect of two natural aroma mosquito repellent compounds, citronella and citronellal. Entomol Res 2005, 35, 117-120. https://doi.org/10.1111/j.1748-5967.2005.tb00146.x
  15. Kim SI, Chang KS, Yang YC, Kim BS, Ahn YJ. Repellency of aerosol and cream products containing fennel oil to mosquitoes under laboratory and field conditions. Pest Manag Sci 2004, 60, 1125-1130. https://doi.org/10.1002/ps.921
  16. Mohammed OB, Hussein HS, Elowni EE. The ant, Pachycondyla sennaarensis (Mayr) as an intermediate host for the poultry cestode, Raillietina tetragona (Molin). Vet Res Commun 1988, 12, 325-327. https://doi.org/10.1007/BF00343251
  17. Lee YJ, Kim EO, Choi SW. Isolation and identification of antioxidant polyphenolic compounds in mulberry (Morus alba L.) seeds. J Korean Soc Food Sci Nutr 2011, 40, 517-524. https://doi.org/10.3746/jkfn.2011.40.4.517
  18. Li WR, Shi QS, Ouyang YS, Chen YB, Duan SS. Antifungal effects of citronella oil against Aspergillus niger ATCC 16404. Appl Microbiol Biotechnol 2013, 97, 7483-7492. https://doi.org/10.1007/s00253-012-4460-y
  19. Lu Y, Shipton FN, Khoo TJ, Wiart C. Antioxidant activity determination of citronellal and crude extracts of Cymbopogon citratus by 3 different methods. Pharmacol Pharm 2014, 5, 395-400. https://doi.org/10.4236/pp.2014.54047
  20. Luangnarumitchai S, Lamlertthon S, Tiyaboonchai W. Antimicrobial activity of essential oils against five strains of Propionibacterium acnes. Warasan Phesatchasat 2007, 34, 60-64.
  21. Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules 2010, 15, 9252-9287. https://doi.org/10.3390/molecules15129252
  22. Moore-Neibel K, Gerber C, Patel J, Friedman M, Ravishankar S. Antimicrobial activity of lemongrass oil against Salmonella enterica on organic leafy greens. J Appl Microbiol 2012, 112, 485-492. https://doi.org/10.1111/j.1365-2672.2011.05222.x
  23. Naik MI, Forma BA, Jaykumar E, Bhat JA. Antibacterial activity of lemongrass (Cymbopogon citratus) oil against some selected pathogenic bacterias. Asian Pac J Trop Med 2010, 3, 535-538. https://doi.org/10.1016/S1995-7645(10)60129-0
  24. NPIC. Oil of citronella general fact sheet. National Pesticide Information Center, Oregon State University, Corvallis, 2013.
  25. Organisation for Economic Co-operation and Development. OECD Guidelines for the Testing of Chemicals, Section 4: Health Effects. Test No. 420: Acute oral toxicity - fixed dose procedure. OECD Publishing, Paris, 2002.
  26. Sekine N, Shibutani S. Chemical structures of p-menthane monoterpenes with special reference to their effect on seed germination and termite mortality. J Wood Sci 2013, 59, 229-237. https://doi.org/10.1007/s10086-013-1327-5
  27. Singh B, Singh PR, Mohanty MK. Toxicity of a plant based mosquito repellent/killer. Interdiscip Toxicol 2012, 5, 184-191.
  28. Thorsell W, Mikiver A, Malander I, Tunon H. Efficacy of plant extracts and oils as mosquito repellents. Phytomedicine 1998, 5, 311-323. https://doi.org/10.1016/S0944-7113(98)80072-X
  29. Tyagi BK, Shahi AK, Kaul BL. Evaluation of repellent activities of Cymbopogon essential oils against mosquito vectors of Malaria, Filariasis and Dengue Fever in India. Phytomedicine 1998, 5, 324-329. https://doi.org/10.1016/S0944-7113(98)80073-1
  30. Zhu BC, Henderson G, Chen F, Fei H, Laine RA. Evaluation of vetiver oil and seven insect-active essential oils against the Formosan subterranean termite. J Chem Ecol 2001, 27, 1617-1625. https://doi.org/10.1023/A:1010410325174

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