Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Protective effect of Hizikia fusiforme on radiation-induced damage in splenocytes

방사선을 조사한 마우스에서 비장세포에 대한 톳의 보호 작용

  • Kim, Areum (Department of Advanced Convergence Technology & Science, Jeju National University) ;
  • Bing, So Jin (Department of Veterinary Medicine, Jeju National University) ;
  • Cho, Jinhee (Department of Veterinary Medicine, Jeju National University) ;
  • Ahn, Ginnae (Department of Marine Bio-Food Sciences, Chonnam National University) ;
  • Lee, Ji-Hyeok (Department of Marine Life Sciences, Jeju National University) ;
  • Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Byung-Gul (Department of Civil Engineering, Jeju National University) ;
  • Jee, Youngheun (Department of Advanced Convergence Technology & Science, Jeju National University)
  • 김아름 (제주대학교 차세대융복합과학기술협동과정) ;
  • 빙소진 (제주대학교 수의과대학 수의학과) ;
  • 조진희 (제주대학교 수의과대학 수의학과) ;
  • 안긴내 (전남대학교 수산해양대학 해양바이오식품학과) ;
  • 이지혁 (제주대학교 해양과학대학 해양생물자원이용공학연구실) ;
  • 전유진 (제주대학교 해양과학대학 해양생물자원이용공학연구실) ;
  • 이병걸 (제주대학교 토목공학과) ;
  • 지영흔 (제주대학교 차세대융복합과학기술협동과정)
  • Received : 2015.02.25
  • Accepted : 2015.03.19
  • Published : 2015.03.31
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Abstract

The immune system is specifically sensitive to oxidative stress induced by ionizing radiation because of its rapid proliferative activity. For this reason, an instructive immune system is one of the best ways to minimize side effects, such immunodeficiency, of gamma radiation. Over the past few decades, several natural plants with antioxidant and immunomodulatory properties have been identified as adjuncts for nontoxic and successful radiotherapy. Hizikia fusiforme extract (HFE) containing plentiful dietary fiber and fucoidan is known for its instructive antioxidant capacity, immunomodulation abilities, and immune activation. In this study, we determined whether HFE protects radiosensitive immune cells from gamma radiation-induced damage. C57BL/6 mice were irradiated with gamma-ray. The effect of HFE on the ionizing radiation damage of immune cells was then evaluated with an MTT assay, 3H-thymidine incorporation assay, and PI staining. We found that HFE stimulated the proliferation of gamma-ray irradiated immune cells without cytotoxic effects. We also observed that HFE not only decreased DNA damage but also reduced gamma radiation-induced apoptosis of the immune cells. Our results suggest that HFE can protect immune cells from gamma-ray damage and may serve as an effective, non-toxic radioprotective agent.

Keywords

References

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