Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic analysis of env and gag gene fragments of bovine leukemia virus identified in cattle from Korea

  • Kim, Yeon-Hee (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Lee, Eun-Yong (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Oem, Jae-Ku (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Kim, Seong-Hee (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Lee, Myoung-Heon (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Lee, Kyoung-Ki (Division of Animal Diseases Diagnosis, Animal and Plant Quarantine Agency) ;
  • Park, Se-Chang (College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2014.10.07
  • Accepted : 2014.12.29
  • Published : 2015.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis. This study was conducted to clarify the molecular characteristics of BLVs obtained from a specific region in Korea. Proviral BLVs were detected in anti-BLV antibody-positive blood samples by PCR. Env and gag fragments were sequenced and compared to previously published reference sequences. Analysis of the env gene sequence revealed that the YI strain was highly similar to genotype 1, including United States and Japanese strains. The gag gene sequence had the highest degree of similarity with a Japanese strain.

Keywords

References

  1. Asfaw Y, Tsuduku S, Konishi M, Murakami K, Tsuboi T, Wu D, Sentsui H. Distribution and superinfection of bovine leukemia virus genotypes in Japan. Arch Virol 2005, 150, 493-505. https://doi.org/10.1007/s00705-004-0433-5
  2. Camargos MF, Pereda A, Stancek D, Rocha MA, dos Reis JKP, Greiser-Wilke I, Leite RC. Molecular characterization of the env gene from Brazilian field isolates of bovine leukemia virus. Virus Genes 2007, 34, 343-350. https://doi.org/10.1007/s11262-006-0011-x
  3. Choudhury B, Finnegan C, Frossard JP, Venables C, Steinbach F. Colostrum replacer and bovine leukemia virus seropositivity in calves. Emerg Infect Dis 2013, 19, 1027-1028. https://doi.org/10.3201/eid1906.121523
  4. Dube S, Abbott L, Dube DK, Dolcini G, Gutierrez S, Ceriani C, Juliarena M, Ferrer J, Perzova R, Poiesz BJ. The complete genomic sequence of an in vivo low replicating BLV strain. Virol J 2009, 6, 120. https://doi.org/10.1186/1743-422X-6-120
  5. Dus Santos MJ, Trono K, Lager I, Wigdorovitz A. Development of a PCR to diagnose BLV genome in frozen semen samples. Vet Microbiol 2007, 119, 10-18. https://doi.org/10.1016/j.vetmic.2006.08.030
  6. Have P, Hoff-Jorgensen R. Demonstration of antibodies against bovine leukemia virus (BLV) by blocking ELISA using bovine polyclonal anti-BLV immunoglobulin. Vet Microbiol 1991, 27, 221-229. https://doi.org/10.1016/0378-1135(91)90149-A
  7. Juliarena MA, Lendez PA, Gutierrez SE, Forletti A, Rensetti DE, Ceriani MC. Partial molecular characterization of different proviral strains of bovine leukemia virus. Arch Virol 2013, 158, 63-70. https://doi.org/10.1007/s00705-012-1459-8
  8. Licursi M, Inoshima Y, Wu D, Yokoyama T, Gonzalez ET, Sentsui H. Provirus variants of bovine leukemia virus in naturally infected cattle from Argentina and Japan. Vet Microbiol 2003, 96, 17-23. https://doi.org/10.1016/S0378-1135(03)00202-5
  9. Lim SI, Jeong W, Tark DS, Yang DK, Kweon CH. Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T−). J Vet Sci 2009, 10, 331-336. https://doi.org/10.4142/jvs.2009.10.4.331
  10. Matsumura K, Inoue E, Osawa Y, Okazaki K. Molecular epidemiology of bovine leukemia virus associated with enzootic bovine leukosis in Japan. Virus Res 2011, 155, 343-348. https://doi.org/10.1016/j.virusres.2010.11.005
  11. Rodriguez SM, Florins A, Gillet N, de Brogniez A, Sanchez-Alcaraz MT, Boxus M, Boulanger F, Gutierrez G, Trono K, Alvarez I, Vagnoni L,Willems L. Preventive and therapeutic strategies for bovine leukemia virus: lessons for HTLV. Viruses 2011, 3, 1210-1248. https://doi.org/10.3390/v3071210
  12. Suh GH, Lee JC, Lee CY, Hur TY, Son DS, Ahn BS, Kim NC, Lee CG. Establishment of a bovine leukemia virus-free dairy herd in Korea. J Vet Sci 2005, 6, 227-230.
  13. Suh GH, Lee CG, Lee CY, Hur TY, Kang SJ, Son DS, Ryu IS, Ahn BS, Kim NC, Joo YS. Prevalence of antibovine Leukemia virus antibodies in dairy and Korean native cattle. J Vet Clin 2003, 20, 172-176.
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28, 2731-2739. https://doi.org/10.1093/molbev/msr121
  15. World Organisation for Animal Health. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). Chap. 2.4.11. Enzootic bovine leukosis. 7th ed. Vol. 1. pp. 721-731, OIE, Paris, 2012.

Cited by

  1. Phylogenetic analysis of env gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan vol.79, pp.5, 2017, https://doi.org/10.1292/jvms.17-0055
  2. Bovine Leukaemia Virus: Current Epidemiological Circumstance and Future Prospective vol.13, pp.11, 2015, https://doi.org/10.3390/v13112167