Journal of electromagnetic engineering and science

The Korean Institute of Electromagnetic Engineering and Science (한국전자파학회)
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Effects of 60-Hz Magnetic Fields on DNA Damage Responses in HT22 Mouse Hippocampal Cell Lines

  • Mun, Gil-Im (Graduate School of Pharmaceutical Sciences, Ewha Womans University) ;
  • Lee, Seungwoo (School of Information and Communication Engineering, Chungbuk National University) ;
  • Kim, Nam (School of Information and Communication Engineering, Chungbuk National University) ;
  • Lee, Yun-Sil (Graduate School of Pharmaceutical Sciences, Ewha Womans University)
  • Received : 2015.04.06
  • Accepted : 2015.05.21
  • Published : 2015.07.31
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Abstract

Previously, we investigated extremely low-frequency magnetic fields (ELF-MFs) on diverse DNA damage responses, such as phosphorylated H2AX (${\gamma}H2AX$), comet tail moments, and aneuploidy production in several non-tumorigenic epithelial or fibroblast cell lines. However, the effect of ELF-MF on DNA damage responses in neuronal cells may not be well evaluated. Here, we investigated the effects of ELF-MF on the DNA damage responses in HT22 non-tumorigenic mouse neuronal cells. Exposure to a 60-Hz, 2 mT ELF-MF did not produce any increased ${\gamma}H2AX$ expression, comet tail moments, or aneuploidy formation. However, 2 mT ELF-MF transiently increased the cell number. From the results, ELF-MF could affect the DNA damage responses differently, depending on the cell lines.

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References

  1. J. Juutilainen and S. Lang, "Genotoxic, carcinogenic and teratogenic effects of electromagnetic fields: introduction and overview," Mutation Research/Reviews in Mutation Research, vol. 387, no. 3, pp. 165-171, 1997. https://doi.org/10.1016/S1383-5742(97)00036-7
  2. J. McCann, F. Dietrich, and C. Rafferty, "The genotoxic potential of electric and magnetic fields: an update," Mutation Research/Reviews in Mutation Research, vol. 411, no. 1, pp. 45-86, 1998. https://doi.org/10.1016/S1383-5742(98)00006-4
  3. J. E. Moulder, "Power-frequency fields and cancer," Critical Reviews in Biomedical Engineering, vol. 26, no. 1-2, pp. 1-116, 1998. https://doi.org/10.1615/CritRevBiomedEng.v26.i1-2.10
  4. J. Juutilainen, T. Kumlin, and J. Naarala, "Do extremely low frequency magnetic fields enhance the effects of environmental carcinogens? A meta-analysis of experimental studies," International Journal of Radiation Biology, vol. 82, no. 1, pp. 1-12, 2006. https://doi.org/10.1080/09553000600577839
  5. Vijayalaxmi and G. Obe, "Controversial cytogenetic observations in mammalian somatic cells exposed to extremely low frequency electromagnetic radiation: a review and future research recommendations," Bioelectromagnetics, vol. 26, no. 5, pp. 412-430, 2005. https://doi.org/10.1002/bem.20111
  6. Vijayalaxmi and T. J. Prihoda, "Genetic damage in mammalian somatic cells exposed to extremely low frequency electro-magnetic fields: a meta-analysis of data from 87 publications (1990-2007)," International Journal of Radiation Biology, vol. 85, no. 3, pp. 196-213, 2009. https://doi.org/10.1080/09553000902748575
  7. H. Yaguchi, M. Yoshida, G. R. Ding, K. Shingu, and J. Miyakoshi, "Increased chromatid-type chromosomal aberrations in mouse m5S cells exposed to power-line frequency magnetic fields," International Journal of Radiation Biology, vol. 76, no. 12, pp. 1677-1684, 2000. https://doi.org/10.1080/09553000050201172
  8. M. Villarini, G. Scassellati-Sforzolini, M. Moretti, and R. Pasquini, "In vitro genotoxicity of terbutryn evaluated by the alkaline single-cell microgel-electrophoresis "comet" assay," Cell Biology and Toxicology, vol. 16, no. 5, pp. 285-292, 2000. https://doi.org/10.1023/A:1026794213308
  9. M. J. Ruiz-Gomez, F. Sendra-Portero, and M. Martinez-Morillo, "Effect of 2.45 mT sinusoidal 50 Hz magnetic field on Saccharomyces cerevisiae strains deficient in DNA strand breaks repair," International Journal of Radiation Biology, vol. 86, no. 7, pp. 602-611, 2010. https://doi.org/10.3109/09553001003734519
  10. H. J. Lee, Y. B. Jin, J. S. Lee, J. I. Choi, J. W. Lee, S. H. Myung, and Y. S. Lee, "Combined effects of 60 Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells," Bioelectromagnetics, vol. 33, no. 3, pp. 207-214, 2012. https://doi.org/10.1002/bem.20700
  11. Y. B. Jin, S. H. Choi, J. S. Lee, J. K. Kim, J. W. Lee, S. C. Hong, S. H. Myung, and Y. S. Lee, "Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression," Radiation and Environmental Biophysics, vol. 53, no. 1, pp. 93-101, 2014. https://doi.org/10.1007/s00411-013-0506-5
  12. Y. B. Jin, G. Y. Kang, J. S. Lee, J. I. Choi, J. W. Lee, S. C. Hong, S. H. Myung, and Y. S. Lee, "Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression," International Journal of Radiation Biology, vol. 88, no. 4, pp. 374-380, 2012. https://doi.org/10.3109/09553002.2012.652724
  13. H. Jin, H. E. Yoon, J. S. Lee, J. K. Kim, S. H. Myung, and Y. S. Lee, "Effects on G2/M phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in L132 nontumorigenic human lung epithelial cells," Korean Journal of Physiology & Pharmacology, vol. 19, no. 2, pp. 119-124, 2015. https://doi.org/10.4196/kjpp.2015.19.2.119
  14. H. E. Yoon, J. S. Lee, S. H. Myung, and Y. S. Lee, "Increased ${\gamma}$-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines," International Journal of Radiation Biology, vol. 90, no. 4, pp. 291-298, 2014. https://doi.org/10.3109/09553002.2014.887866
  15. M. N. Hong, N. K. Han, H. C. Lee, Y. K. Ko, S. G. Chi, Y. S. Lee, Y. M. Gimm, S. H. Myung, and J. S. Lee, "Extremely low frequency magnetic fields do not elicit oxidative stress in MCF10A cells," Journal of Radiation Research, vol. 53, no. 1, pp. 79-86, 2012. https://doi.org/10.1269/jrr.11049
  16. H. Lai and N. P. Singh, "Acute exposure to a 60 Hz magnetic field increases DNA strand breaks in rat brain cells," Bioelectromagnetics, vol. 18, no. 2, pp. 156-165, 1997. https://doi.org/10.1002/(SICI)1521-186X(1997)18:2<156::AID-BEM8>3.0.CO;2-1
  17. B. M. Svedenstal, K. J. Johanson, M. O. Mattsson, and L. E. Paulsson, "DNA damage, cell kinetics and ODC activities studied in CBA mice exposed to electromagnetic fields generated by transmission lines," In vivo (Athens, Greece), vol. 13, no. 6, pp. 507-513, 1998.
  18. B. M. Svedenstal, K. J. Johanson, and K. H. Mild, "DNA damage induced in brain cells of CBA mice exposed to magnetic fields," In vivo (Athens, Greece), vol. 13, no. 6, pp. 551-552, 1999.
  19. B. Yokus, M. Z. Akdag, S. Dasdag, D. U. Cakir, and M. Kizil, "Extremely low frequency magnetic fields cause oxidative DNA damage in rats," International Journal of Radiation Biology, vol. 84, no. 10, pp. 789-795, 2008. https://doi.org/10.1080/09553000802348203
  20. I. H. Ismail, T. I. Wadhra, and O. Hammarsten, "An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans," Nucleic Acids Research, vol. 35, no. 5, pp. e36, 2007. https://doi.org/10.1093/nar/gkl1169