Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
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Carbohydrate Fermentation Character of Bifidobacteria and Lactobacilli isolated from Feces of the Adult Women supplied with Goat Milk

산양유를 섭취한 성인 여성의 분변에서 분리한 Bifidobacteria와 Lactobacilli의 탄수화물 발효특성

  • Received : 2015.05.04
  • Accepted : 2015.05.19
  • Published : 2015.06.30
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Abstract

The objective of this study was to investigate the effects of consuming goat milk on the bacterial counts (colony forming units [CFU]) in adult women and to evaluate the carbohydrate fermentation capacity of bifidobacteria and lactobacilli isolated from their feces. Adult women who consumed goat milk (treatment group) had relatively higher CFU of bifidobacteria than did the control group, and the difference was significant (p<0.05) after 8 weeks. In total, 13 strains isolated from the feces of women in the treatment group were identified using 16S rRNA sequencing as Bifidobacterium adolescentis, B. longum, B. pseudocatenulatum, B. dentium, and Lactobacillus sakei. Similarly, 12 strains isolated from the feces of women in the control group included B. adolescentis, B. longum, L. ruminis, L. sakei, and B. pseudocatenulatum. All isolated bifidobacteria and lactobacilli fermented goat milk oligosaccharide and lactulose. All 7 strains of B. adolescentis fermented fructooligosaccharides, and 3 of the 4 B. pseudocatenulatum strains, 2 of the 3 L. sakei strains, and 1 of the 7 B. longum strains fermented fructooligosaccharides.

산양유을 섭취한 성인의 분변 내 bifidobacteria의 증식 효과 및 분변에서 분리된 bifidobacteria와 lactobacilli의 당 발효 능력을 조사하였다. 1. 산양유를 섭취한 처리군이 대조군보다 bifidobacteria 세균수가 상대적으로 높았으며, 8주에서 95% 신뢰한계에서 유의성이 있었다. 2. 분변에서 분리한 균주들을 13 균주의 16S rRNA 염기서 열로 동정한 결과, 산양유를 섭취한 처리구는 B. adolescentis, B. longum, B. pseudocatenulatum, B. dentium, L. sakei 순으로 분리되었다. 대조군의 12 균주는 B. adolescentis, B. longum, L. ruminis, L. sakei, B. pseudocatenulatum 순으로 분리되었다. 3. 산양유 올리고당과 lactulose는 모든 bifidobacteria 및 lactobacilli 균주가 발효하였다. Fructooligosaccaride는 B. adolescentis 7 균주 모두 발효하였다. 조사한 B. pseudocatenulatum 4 균주, L. sakei 3 균주, B. longum 7 균주 중에서 각각 3, 2, 1 균주가 fructooligosaccharide를 발효하였다.

Keywords

References

  1. Bezkorovainy, A. 1989. Nutrition and metabolism of bifidobacteria. In: Biochemistry and physiology of bifidobacteria. Bezkorovainy, A., Miller-Catchpole, R., eds. CRC Press, Boca Raton.
  2. Biavati, B., Castagnoli, P. and Trovatelli, L. D. 1986. Species of the genus Bifidobacterium in the feces of human adults. Microbiologica (Bologna) 9:39-45.
  3. De Vuyst, L. 2002. Inhibitory effects of probiotic lactic acid bacteria. Probiotics-accompanying measure: Flair-flow Europe IV, Cracow, Poland, 28-32.
  4. Finegold, S. M., Attebery, H. R. and Sutter, V. L. 1974. Effect of diet on human fecal flora: Comparison of Japanese and American diets. Am. J. Clin. Nutr. 27:1456-1469. https://doi.org/10.1093/ajcn/27.12.1456
  5. Fiordaliso, M., Kok, N., Desaqer, J. P., Goethals, F., Deboyser, D., Roberfroid, M. and Delzenne, N. 1995. Dietaryoligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30:163-167. https://doi.org/10.1007/BF02538270
  6. Fooks, L. J., Fuller, R. and Gibson, G. R. 1999. Prebiotics, probiotics and human gut microbiology. Inter. Dairy J. 9:53-61. https://doi.org/10.1016/S0958-6946(99)00044-8
  7. Higa, H., and Paulson, J. 1985. Sialylation of glycoprotein oligosaccharides with N-acetyl-, N-glycolyl-and N-Odiacetylneuraminic acids. J. Biological Chem. 200:8838-8849.
  8. Imamura, L., Hisamitsu, K. and Kobashi, K. 1994. Purification and characterisation of ${\beta}$- fructofuranosidase from Bifidobacterium infantis. Biol. Pharm. Bull. 17:596-602. https://doi.org/10.1248/bpb.17.596
  9. Kaplan, H. and Hutkins, R. W. 2000. Fermentation of fructooligosaccharides by lactic acid bacteria and bifidobacteria. Appli. Environ. Microbiol. 66:2682-2684. https://doi.org/10.1128/AEM.66.6.2682-2684.2000
  10. Kleessen, B., Hartmann, L. and Blaut, M. 2003. Fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats. Br. J. Nutr. 89:597-606. https://doi.org/10.1079/BJN2002827
  11. Kleessen, B., Stoof, G., Proll, J., Schmiedl, D., Noack, J., and Blaut, M. 1997. Feeding resistant starch affects fecal and cecal microflora and short-chain fatty acid in rat. J. Anim. Sci. 75:2453-2462. https://doi.org/10.2527/1997.7592453x
  12. Koon, Y. S. and Lee, S. Y. 2002. Effects of bifidobacteria and oligosaccharide on the quality attributes of frozen soy yogurts. Korean J. Soc. Food Cookery Sci. 18:43-50.
  13. Langlands, S. J., Hopkins, M. J., Coleman, N. and Cummings, J. H. 2004. Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut 53:1610-1616. https://doi.org/10.1136/gut.2003.037580
  14. Martinez-Ferez, A., Rudloff, S., Guadix, A., Henkel, C. A., Pohlentz, G., Boza, J. J., Guadix, E. M. and Kunz, C. 2006. Goats' milk as a natural source of lactose-derived oligosaccharides : Isolation by membrane technology. Int. Dairy J. 16:173-181. https://doi.org/10.1016/j.idairyj.2005.02.003
  15. Matsuki, T., Watanabe, K., Tanaka, R., Fukuda, M. and Oyaizu, H. 1999. Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Appl. Enviroment. Microbiol. 65:4506-4512. https://doi.org/10.1128/AEM.65.10.4506-4512.1999
  16. Moore, W. E. C. and Holdeman, L. V. 1974. Human fecal flora: The normal flora of 20 Japanese-Hawaiians. Appl. Microbiol. 27:961-979.
  17. Park, Y., Juarez, M., Ramos, M. and Haenlein, G. 2007. Physico-chemical characteristics of goat and sheep milk. Small Ruminant Res. 68:88-113. https://doi.org/10.1016/j.smallrumres.2006.09.013
  18. Perrin, S., Warchol, M., Grill, J. P. and Schneider, F. 2001. Fermentations of fructo-oligosaccharides and their components by Bifidobacterium infantis ATCC 15697 on batch culture in semi-synthetic medium. J. Appli. Microbiol. 90:859-865. https://doi.org/10.1046/j.1365-2672.2001.01317.x
  19. Raynal-Ljutovac, K., Lagriffoul, G., Paccard, P., Guillet, I. and Chilliard, Y. 2008. Composition of goat and sheep milk products: An update. Small Ruminant Res. 79:57-72. https://doi.org/10.1016/j.smallrumres.2008.07.009
  20. Roberfroid, M. B. 1998. Prebiotics and synbiotics: Concepts and nutritional properties. Brit. J. Nutr. 80: Suppl. 2, S197-S202. https://doi.org/10.1017/S0007114500006024
  21. Russell D., Ross, R., Fitzgerald, G. and Stanton, C. 2011. Metabolic activities and probiotic potential of bifidobacteria. Inter. J. Food Microbiol. 149:88-105. https://doi.org/10.1016/j.ijfoodmicro.2011.06.003
  22. Scardovi, V. and Crociani, F. 1974. Bifidobacterium catenulatum, Bifidobacterium dentium, and Bifidobacterium angulatum: three new species and their deoxyribonucleic acid homology relationships. Int. J. Syst. Bacteriol. 24:6-20. https://doi.org/10.1099/00207713-24-1-6
  23. Scardovi, V., Trovatelli, L. D., Biavati, B. and Zani, G. 1979. Bifidobacterium cuniculi, Bifidobacterium choerinum, Bifidobacterium boum, and Bifidobacterium pseudocatenulatum: four new species and their deoxyribonucleic acid homology relationships. Int. J. Syst. Bacteriol. 29:291-311. https://doi.org/10.1099/00207713-29-4-291
  24. Silanikove, N., Leitner, G., Merin, U. and Prosser, C. G. 2010. Recent advances in exploiting goat's milk: Quality, safety and production aspects. Small Ruminant Res. 89:110-124. https://doi.org/10.1016/j.smallrumres.2009.12.033
  25. Urashima, T. and Taufik, E. 2010. Oligosaccharides in milk: Their benefits and future utilization. Media Peternakan, (December):189-197.