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Biomass and oil content of microalgae under mixotrophic conditions

  • Choi, Hee-Jeong (Department of Environmental Engineering, Catholic Kwandong University) ;
  • Lee, Seung-Mok (Department of Environmental Engineering, Catholic Kwandong University)
  • 투고 : 2014.07.15
  • 심사 : 2014.12.27
  • 발행 : 2015.03.31

초록

The growth of the algae strains Neochloris oleabundans, Botryococcus Braunii and Dunaliella sp. under mixotrophic conditions in the presence of different concentrations of crude glycerol was evaluated with the objective of increasing the biomass growth and algal oil content. A high biomass concentration was characteristic of these strains when grown on crude glycerol compared to autotrophic growth, and 5 g/L glycerol yielded the highest biomass concentration for these strains. Mixotrophic conditions improved both the growth of the microalgae and the accumulation of triacylglycerols (TAGs). The maximum amount of TAGs in the algal strains was obtained in the 5 g/L glycerol growth medium. The fatty acid profiles of the oil for the cultures met the necessary requirements and are promising resources for biofuel production.

키워드

참고문헌

  1. Pulz O, Grass W. Valuable products from biotechnology of microalgae. Appl. Microbiol. Biotechnol. 2004;65:635-648. https://doi.org/10.1007/s00253-004-1647-x
  2. Borowitzka MA, Moheimani NR. Sustainable biofuels from algae. Mitig. Adapt. Strateg. Glob. Chang. 2013;18:13-25. https://doi.org/10.1007/s11027-010-9271-9
  3. Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial applications of microalgae. J. Biosci. Bioeng. 2006;101:87-96. https://doi.org/10.1263/jbb.101.87
  4. Rudolfi L, Chini Zittelli G, Bassin N, et al. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol. Bioeng. 2009;102:100-112. https://doi.org/10.1002/bit.22033
  5. Xu H, Miao X, Wu Q. High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J. Biotechnol. 2006;126:499-507. https://doi.org/10.1016/j.jbiotec.2006.05.002
  6. Pittman JK, Dean AP, Osundeko O. The potential of sustainable algal biofuel production using wastewater resources. Bioresour. Technol. 2011;102:17-25. https://doi.org/10.1016/j.biortech.2010.06.035
  7. Chen GQ, Chen F. Growing phototrophic cells without light. Biotechnol. Lett. 2006;28:607-616. https://doi.org/10.1007/s10529-006-0025-4
  8. Qiao H, Wang G, Zhang X. Isolation and characterization of Chlorella sorokiniana GXNN01(Chlorophyta) with the properties of heterotrophic and microaerobic growth. J. Phycol. 2009;45:1153-1162. https://doi.org/10.1111/j.1529-8817.2009.00736.x
  9. Yang C, Hua Q, Shimizu K. Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions. Biochem. Eng. J. 2000;6:87-102. https://doi.org/10.1016/S1369-703X(00)00080-2
  10. Zhang H, Wang W, Li Y, Yang W, Shen G. Mixotrophic cultivation of Botryococcus braunii. Biomass Bioenergy. 2011;35: 1710-1715. https://doi.org/10.1016/j.biombioe.2011.01.002
  11. Alkhamis Y, Qin JG. Cultivation of isochrysis galbana in phototrophic, heterotrophic, and mixotrophic conditions. BioMed Res. Int. 2013;2013:983465.
  12. Chojnacka K, Noworyta A. Evaluation of Spirulina sp. growth in photoautotrophic, heterotrophic and mixotrophic cultures. Enzyme Microb. Technol. 2004;34:461-465. https://doi.org/10.1016/j.enzmictec.2003.12.002
  13. Bouarab L, Dauta A, Loudiki M. Heterotrophic and mixotrophic growth of Micractinium pusillum Fresenius in the presence of acetate and glucose: effect of light and acetate gradient concentration. Water Res. 2004;38:2706-2712. https://doi.org/10.1016/j.watres.2004.03.021
  14. Thompson JC, He BB. Characterization of crude glycerol from biodiesel production from multiple feedstocks. Appl. Eng. Agric. 2006;22:261-265. https://doi.org/10.13031/2013.20272
  15. Johnson DT, Taconi KA. The glycerol glut: Options for the value-added conversion of crude glycerol resulting from biodiesel production. Environ. Prog. 2007;26:338-348. https://doi.org/10.1002/ep.10225
  16. Yang F, Hanna MA, Sun R. Value-added uses for crude glycerol- a byproduct of biodiesel production. Biotechnol. Biofuels. 2002;5:1-10.
  17. Chi Z, Pyle D, Wen Z, Frear C, Chen S. A laboratory study of producing docosahexaenoic acid from biodiesel-water glycerol by microalgal fermentation. Process Biochem. 2007;42: 1537-1545. https://doi.org/10.1016/j.procbio.2007.08.008
  18. Pyle DJ, Garcia RA, Wen Z. Producitng docosahexaenoic acid (DHA)-rich algae from biodiesel-derived crude glycerol: effects of impurities on DHA production and algal biomass composition. J. Agric. Food Chem. 2008;56:3933-3939. https://doi.org/10.1021/jf800602s
  19. Choi HJ, Lee JM, Lee SM. A novel optical panel photobioreactor for cultivation of microalgae. Water Sci. Technol. 2013;67:2543-2548. https://doi.org/10.2166/wst.2013.128
  20. Mitra D, van Leenwen J, Lamsal B. Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products. Algal Res. 2012;1:40-48. https://doi.org/10.1016/j.algal.2012.03.002
  21. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 1959;37:911-917. https://doi.org/10.1139/o59-099
  22. Sobczuk TM, Chisti Y. Potential fuel oils from the microalga Choricystis minor. J. Chem. Technol. Biotechnol. 2010;85: 100-108. https://doi.org/10.1002/jctb.2272
  23. Stehfest K, Toepel J, Wilhelm C. The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiol. Biochem. 2005;43:717-726. https://doi.org/10.1016/j.plaphy.2005.07.001
  24. Andruleviciute V, Makareviciene V, Skorupskaite V, Gumbyte M. Biomass and oil content of Chlorella sp., Haematococcus sp., Nannochloris sp. and Scenedesmus sp. under mixotrophic growth conditions in the presence of technical glycerol. J. Appl. Phycol. 2014;26:83-90. https://doi.org/10.1007/s10811-013-0048-x
  25. Ceron Garcia MC, Fernandez Sevilla JM, Acien Fernandez FG, Molina Grima E, Garcia Camacho F. Mixotrophic growth of Phaeodactrylum tricornutum on glycerol: growth rate and fatty acid profile. J. Appl. Phycol. 2000;12:239-248. https://doi.org/10.1023/A:1008123000002
  26. Liang Y, Sarkany N, Cui Y. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol. Lett. 2009;31:1043-1049. https://doi.org/10.1007/s10529-009-9975-7
  27. Liang Y, Sarkany N, Cui Y, Blackburn JM. Batch stage study of lipid production from crude glycerol derived from yellow grease or animal fats through microalgal fermentation. Bioresour. Technol. 2010;101:6745-6750. https://doi.org/10.1016/j.biortech.2010.03.087
  28. Perez-Garcia O, de-Bashan LE, Hernandez JP, Bashan Y. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense. J. Phycol. 2010;46:800-812. https://doi.org/10.1111/j.1529-8817.2010.00862.x
  29. Chen YH, Walker TH. Biomass and lipid production of heterotrophic microalgae Chlorella protothecoides by using biodiesel- derived crude glycerol. Biotechnol. Lett. 2011;33:1973-1983. https://doi.org/10.1007/s10529-011-0672-y
  30. Ramos MJ, Fernandez CM, Casas A, Rodriguez L, Perez A. Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour. Technol. 2009;100:261-268. https://doi.org/10.1016/j.biortech.2008.06.039
  31. Kong WB, Yang H, Cao YT, Song H, Hua SF, Xia CG. Effects of glycerol and glucose on the enhancement of biomass, lipid and soluble carbohydrate production by Chlorella vulgaris in mixotrophic cultures. Food Technol. Biotechnol. 2013;51:62-69.

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