DOI QR코드

DOI QR Code

양송이 재배사 공기 중에 존재하는 세균조사

Investigation of bacteria in indoor air of a greenhouse for button mushroom cultivation

  • 권혁우 (단국대학교 미생물학과) ;
  • 최민아 (단국대학교 미생물학과) ;
  • 오연이 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 김성환 (단국대학교 미생물학과)
  • Kwon, Hyuk Woo (Department of Microbiology, Dankook University) ;
  • Choi, Min Ah (Department of Microbiology, Dankook University) ;
  • Oh, Youn-Lee (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University)
  • 투고 : 2015.03.02
  • 심사 : 2015.03.25
  • 발행 : 2015.03.31

초록

양송이는 재배사라는 밀폐된 환경에서 재배되는 바 양질의 생산을 위해서는 재배사 내 환경에 대한 정보가 요구된다. 본 연구는 양송이 재배사내 실내환경에 대한 기초 정보를 얻고자 수행되었다. 재배사 내 온도와 습도는 $19.75{\pm}0.35^{\circ}C$, $87{\pm}3.67%$로 세균이 서식할 수 있는 환경조건이었으며, 부유세균의 농도는 $3.84{\times}10^3cfu/m^3$로서 환경부에서 고시한 실내공기질 오염유지 기준치인 $8.0{\times}10^2cfu/m^3$을 초과하는 수치였다. 분리된 부유세균은 Advenella kashmirensis, Bacillus vietnamensism, B. licheniformis, Burkholderia sordidicola, Fictibacillus phosphorivorans, Lysobacter daejeonensis, Microbacterium esteraromaticum, Pseudomonas aeruginosa, P. protegens, P. gessardii, P. mosseli 등 7속 11종으로 동정되었다.

Since button mushroom (Agaricus bisporus) cultivation is performed in closed environment, the understanding of indoor environment becomes essential for the quality and quantitative production of the greenhouse-grown mushroom. To generate information on indoor environmental factors affecting on fruiting body quality, we investigated temperature, humidity, and bacterial concentration and species in a greenhouse located in Buyeo, Chungnam Province. Temperature and humidity were recorded as $19.75{\pm}0.35^{\circ}C$ and $87{\pm}3.67%$, respectively. The total concentration of bacteria was measured as $3.84{\times}10^3CFU/M^3$. Advenella kashmirensis, Bacillus vietnamensism, B. licheniformis, Burkholderia sordidicola, Fictibacillus phosphorivorans, Lysobacter daejeonensis, Microbacterium esteraromaticum, Pseudomonas aeruginosa, P. protegens, P. gessardii, P. mosseli were identified from indoor air of the greenhouse.

키워드

참고문헌

  1. Adams JDW, Frostick LE. 2008. Investigating microbial activities in compost using mushroom (Agaricus bisporus) cultivation as an experimental system. Bioresour Technol. 99:1097-1102. https://doi.org/10.1016/j.biortech.2007.02.019
  2. Bech K. 1973. Preparing for productive commercial compost as a selective growing medium for Agaricus bisporus (Lange) Singer. Mushroom Sci. 10(2):77-83.
  3. Blue SR, Singh VR, Saubolle MA. 1995. Bacillus licheniformis bacteremia: five cases associated with indwelling central venous catheters. Clin Infect Dis. 20:629-633 https://doi.org/10.1093/clinids/20.3.629
  4. Braaksma A, Schaap DJ. 1996. Protein analysis of the common mushroom Agaricus bisporus. Postharvest Bio Technol. 7:119-127. https://doi.org/10.1016/0925-5214(95)00034-8
  5. Dales RE, Zwanenburg H, Burnett R, Franklin CA. 1991. Respiratory health effects of home dampness and molds among child. Am J Epidemiol. 134(2):196-203. https://doi.org/10.1093/oxfordjournals.aje.a116072
  6. Govan JRW, Deretic V. 1996. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev. 60(3):539-574.
  7. Jeong SC, Jeong YTm Yang BK, Islam R, Koyyalamudi SR, Pang G, Cho KY, Song CH. 2010. White button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats. Nutr Res. 30:49-56. https://doi.org/10.1016/j.nutres.2009.12.003
  8. Kim JY, Kwon HW, Jang S, Choi MA, Ko HK, Kim SH. 2014. A study of bacterial concentration and species in the indoor air of green houses used for shiitake cultivation. J Odor Indoor Envirion. 13(4):1-6. https://doi.org/10.15250/joie.2014.13.1.1
  9. Kiuouasu K, Osamu K, Teiji S, Maria O, Micheal AG, Dara WF, Thomas RM, Jeanine PWK. 1999. Pathogenesis of septic shock in Pseudomonas aeruginosa pneumonia. J Clin Invest. 104:743-750. https://doi.org/10.1172/JCI7124
  10. Koyyalamudi SR, Jeong SC, Song CH, Cho LY, Pang G. 2009. Vitamin D2 formation and bioavailability from Agaricus bisprous button mushrooms treated with ultraviolet irradiation. J Agric Food Chem. 57: 3351-3355. https://doi.org/10.1021/jf803908q
  11. Lane DJ. 1991. 16S/23S rRNA sequencing, pp. 115-175, In Stackebrndt E, Goodfellow (ed.), Nucleic acid techniques in bacterial systematics. New York, NY: John Wiley and Sons Press. United Kingdom.
  12. Malika E, Loic C, Sophie V, Daniel I, Henri L. 1997. Pseudomonas monteilii sp. nov., isolated from clinical specimens. Int J Syst Bacteriol. 47(3):846-852. https://doi.org/10.1099/00207713-47-3-846
  13. Randle PH, Hayes WA. 1972. Progress in experimentation on the efficiency of composting and compost. Mushroom Sci. 8:789-795.
  14. Ren P, Jankun TM, Leaderer BP. 1999. Comparisons of seasonal fungal prevalence in indoor and outdoor air and in house dusts of dwellings in one Northeast American county. J Expo Anal Environ Epidemiol. 99(9):560-568.
  15. Ross RC, Harris PJ. 1983. The signigicance of thermophilic fungi in mushroom compost preparation. Sci Hortic. 20:61-70. https://doi.org/10.1016/0304-4238(83)90112-7
  16. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 4:406-425.
  17. Siersted HC, Gravesen S. 1993. Extrinsic allergic alveolitis after exposure to the yeast Rhodotorula rubra. Allergy. 48(4):298-299. https://doi.org/10.1111/j.1398-9995.1993.tb00734.x
  18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular Evolutionary genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 28:2731-2739. https://doi.org/10.1093/molbev/msr121