Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effects of Bambusae Caulis in Taeniam Extract on the UVB-induced Cell Death, Oxidative Stress and Matrix Metalloproteinase 1 Expression in Keratinocytes

각질세포에서 자외선B가 유도한 세포 사멸, 산화적 스트레스 및 matrix metalloproteinase 1 발현에 대한 죽여추출물의 영향

  • Seok, Jin Kyung (Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine) ;
  • Kwak, Jun Yup (Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine) ;
  • Seo, Hyeong Ho (Ruby Crown Co., Ltd.) ;
  • Suh, Hwa Jin (Gyeongbuk Natural Color Industry Institute) ;
  • Boo, Yong Chool (Department of Molecular Medicine, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine)
  • 석진경 (경북대학교 의학전문대학원 분자의학교실, 세포기질연구소) ;
  • 곽준엽 (경북대학교 의학전문대학원 분자의학교실, 세포기질연구소) ;
  • 서형호 ((주)루비크라운) ;
  • 서화진 ((재)경북천연염색산업연구원) ;
  • 부용출 (경북대학교 의학전문대학원 분자의학교실, 세포기질연구소)
  • Received : 2014.10.20
  • Accepted : 2015.01.09
  • Published : 2015.03.31
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Abstract

Ultraviolet radiation (UV) is a major cause of skin photoaging, and effective UV protecting agents are needed for the skin health and beauty. This study was undertaken to examine the effects of Bambusae caulis in Taeniam extract (BCTE) on UVB-induced cell death, oxidative stress and matrix metalloproteinase 1 (MMP1) expression in cell-based assays. HaCaT human keratinocytes were exposed to UVB in the presence of BCTE at different concentrations and resulting changes in cell viability and biochemical events were determined. The results showed that BCTE enhanced the viabilities of UVB-exposed cells, and attenuated apoptotic events such as cleavage of procaspase 3 to its active form, and the increase of Bax to Bcl-2 ratios. BCTE also attenuated the reactive oxygen generation and lipid peroxidation in cells exposed to UVB. Additionally, it attenuated the expression of matrix metalloproteinase 1 and the phosphorylation of c-Jun N-terminal kinase stimulated by UVB. Conclusively, the present study demonstrated that BCTE pro tected skin cells from the UVB-induced cell death, oxidative stress and MMP1 expression, suggesting its potential use as a cosmetic ingredient mitigating some features of the skin photoaging.

자외선은 피부 광노화의 주요 요인이며, 효과적인 자외선 차단제가 피부의 건강과 아름다움을 위해 필요하다. 본 연구는 세포 실험을 통하여 자외선에 의해 유도된 세포 사멸, 산화적 스트레스, matrix metalloproteinase 1 발현에 미치는 죽여추출물의 영향을 알아보고자 수행하였다. HaCaT 인간 각질세포를 여러 농도의 죽여추출물 유무 조건에서 자외선을 조사하고 세포의 생존율과 생화학적 과정들의 변화를 분석하였다. 죽여추출물은 자외선을 조사한 세포의 생존율을 증진시켰고, procaspase 3가 활성화 형태로 절단되고, Bax/Bcl-2 비율이 증가하는 세포 자살 과정을 완화시켰다. 죽여추출물은 자외선에 노출된 세포에서 활성산소의 발생과 지질 과산화도 감소시켰다. 또한 죽여추출물은 자외선에 의해 자극된 matrix metalloproteinase 1의 발현과 c-Jun N-terminal kinase의 인산화를 억제하였다. 본 연구는 죽여추출물이 자외선에 의한 세포 사멸, 산화적 스트레스, 그리고 matrix metalloproteinase 1 발현을 억제함을 보여 주었으며, 이 추출물이 피부 광노화의 일부 현상을 억제하는 화장품 원료로 유용함을 시사하였다.

Keywords

References

  1. J. H. Rabe, A. J. Mamelak, P. J. McElgunn, W. L. Morison, and D. N. Sauder, Photoaging: mechanisms and repair, J. Am. Acad. Dermatol., 55(1), 1 (2006). https://doi.org/10.1016/j.jaad.2005.05.010
  2. C. Antoniou, M. G. Kosmadaki, A. J. Stratigos, and A. D. Katsambas, Photoaging: prevention and topical treatments, Am. J. Clin. Dermatol., 11(2), 95 (2010). https://doi.org/10.2165/11530210-000000000-00000
  3. K. Scharffetter-Kochanek, P. Brenneisen, J. Wenk, G. Herrmann, W. Ma, L. Kuhr, C. Meewes, and M. Wlaschek, Photoaging of the skin from phenotype to mechanisms, Exp. Gerontol., 35(3), 307 (2000). https://doi.org/10.1016/S0531-5565(00)00098-X
  4. B. Kozma and M. J. Eide, Photocarcinogenesis: an epidemiologic perspective on ultraviolet light and skin cancer, Dermatol. Clin., 32(3), 301 (2014). https://doi.org/10.1016/j.det.2014.03.004
  5. C. H. Lee, S. B. Wu, C. H. Hong, H. S. Yu, and Y. H. Wei, Molecular Mechanisms of UV-Induced Apoptosis and Its Effects on Skin Residential Cells: The Implication in UV-Based Phototherapy, Int. J. Mol. Sci., 14(3), 6414 (2013). https://doi.org/10.3390/ijms14036414
  6. N. Pustisek and M. Situm, UV-radiation, apoptosis and skin, Coll. Antropol., 35, 339 (2011).
  7. L. A. Sitailo, S. S. Tibudan, and M. F. Denning, Activation of caspase-9 is required for UV-induced apoptosis of human keratinocytes, J. Biol. Chem., 277(22), 19346 (2002). https://doi.org/10.1074/jbc.M200401200
  8. G. R. Aitken, J. R. Henderson, S. C. Chang, C. J. McNeil, and M. A. Birch-Machin, Direct monitoring of UV-induced free radical generation in HaCaT keratinocytes, Clin. Exp. Dermatol., 32(6), 722 (2007). https://doi.org/10.1111/j.1365-2230.2007.02474.x
  9. M. Podda, M. G. Traber, C. Weber, L. J. Yan, and L. Packer, UV-irradiation depletes antioxidants and causes oxidative damage in a model of human skin, Free Radic. Biol. Med., 24(1), 55 (1998). https://doi.org/10.1016/S0891-5849(97)00142-1
  10. N. A. Soter, Acute effects of ultraviolet radiation on the skin, Semin. Dermatol., 9(1), 11 (1990).
  11. F. Afaq and H. Mukhtar, Effects of solar radiation on cutaneous detoxification pathways, J. Photochem. Photobiol. B, 63(1-3), 61 (2001). https://doi.org/10.1016/S1011-1344(01)00217-2
  12. V. M. Kahari and U. Saarialho-Kere, Matrix metalloproteinases in skin, Exp. Dermatol., 6(5), 199 (1997). https://doi.org/10.1111/j.1600-0625.1997.tb00164.x
  13. W. Bode, C. Fernandez-Catalan, H. Tschesche, F. Grams, H. Nagase, and K. Maskos, Structural properties of matrix metalloproteinases, Cell Mol. Life Sci., 55(4), 639 (1999). https://doi.org/10.1007/s000180050320
  14. K. Scharffetter, M. Wlaschek, A. Hogg, K. Bolsen, A. Schothorst, G. Goerz, T. Krieg, and G. Plewig, UVA irradiation induces collagenase in human dermal fibroblasts in vitro and in vivo, Arch. Dermatol. Res., 283(8), 506 (1991). https://doi.org/10.1007/BF00371923
  15. P. Brenneisen, J. Oh, M. Wlaschek, J. Wenk, K. Briviba, C. Hommel, G. Herrmann, H. Sies, and K. Scharffetter-Kochanek, Ultraviolet B wavelength dependence for the regulation of two major matrix-metalloproteinases and their inhibitor TIMP-1 in human dermal fibroblasts, Photochem. Photobiol., 64(4), 649 (1996). https://doi.org/10.1111/j.1751-1097.1996.tb03119.x
  16. K. H. Park and M. W. Lee, Anti-oxidative, anti-inflammatory and whitening effects of phenolic compounds from Bambusae Caulis in Liquamen, Nat. Prod. Res., 26(18), 1687 (2012). https://doi.org/10.1080/14786419.2011.593517
  17. Y. M. Lee, Y. K. Kim, K. H. Kim, S. J. Park, S. J. Kim, and J. H. Chung, A novel role for the TRPV1 channel in UV-induced matrix metalloproteinase (MMP)-1 expression in HaCaT cells, J. Cell Physiol., 219(3), 766 (2009). https://doi.org/10.1002/jcp.21729
  18. M. R. Iannacone, M. C. Hughes, and A. C. Green, Effects of sunscreen on skin cancer and photoaging, Photodermatol. Photoimmunol. Photomed., 30(2-3), 55 (2014). https://doi.org/10.1111/phpp.12109
  19. J. F. Nash and P. R. Tanner, Relevance of UV filter/sunscreen product photostability to human safety, Photodermatol. Photoimmunol. Photomed., 30(2-3), 88 (2014). https://doi.org/10.1111/phpp.12113
  20. S. Kim and K. Choi, Occurrences, toxicities, and ecological risks of benzophenone-3, a common component of organic sunscreen products: a mini-review, Environ. Int., 70, 143 (2014). https://doi.org/10.1016/j.envint.2014.05.015
  21. J. Park, J. K. Seok, H. J. Suh, and Y. C. Boo, Gardenia jasminoides extract attenuates the UVB-induced expressions of cytokines in keratinocytes and indirectly Inhibits matrix metalloproteinase-1 expression in human dermal fibroblasts, Evid. Based Complement Alternat. Med., 2014, 429246 (2014).
  22. S. M. An, S. J. Lee, J. S. Koh, K. Park, and Y. C. Boo, Effects of plant extract-containing creams on UVB radiation-induced inflammatory responses in mice, J. Soc. Cosmet. Sci. Korea, 36(4), 271 (2010).
  23. M. J. Lee, M. J. Kim, Y. S. Song, Y. O. Song, and G. S. Moon, Bamboo culm extract supplementation elevates HDL-cholesterol and ameliorates oxidative stress in C57BL/6 mice fed atherogenic diet, J. Med. Food, 11(1), 69 (2008). https://doi.org/10.1089/jmf.2007.009
  24. J. Jiao, Y. Zhang, D. Lou, and X. Wu, Antihyperlipidemic and antihypertensive effect of a triterpenoid-rich extract from bamboo shavings and vasodilator effect of friedelin on phenylephrine- induced vasoconstriction in thoracic aortas of rats, Phytother. Res., 21(12), 1135 (2007). https://doi.org/10.1002/ptr.2223
  25. Y. Zhang, X. Yao, and B. Bao, Anti-fatigue activity of a triterpenoid-rich extract from Chinese bamboo shavings (Caulis bamfusae in taeniam), Phytother. Res., 20(10), 872 (2006). https://doi.org/10.1002/ptr.1965
  26. J. Sun, J. Yu, P. C. Zhang, F. Tang, Y. D. Yue, Y. N. Yang, Z. M. Feng, and X. F. Guo, Isolation and identification of lignans from Caulis Bambusae in Taenia with antioxidant properties, J. Agric. Food Chem., 61(19), 4556 (2013). https://doi.org/10.1021/jf4003686
  27. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med., 26(9-10), 1231 (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
  28. J. Xie and K. M. Schaich, Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity, J. Agric. Food Chem., 62(19), 4251 (2014). https://doi.org/10.1021/jf500180u
  29. M. Mihara and M. Uchiyama, Determination of malonaldehyde precursor in tissues by thiobarbituric acid test, Anal. Biochem., 86(1), 271 (1978). https://doi.org/10.1016/0003-2697(78)90342-1
  30. L. Liu, B. Lu, D. Xia, and Y. Zhang, Evaluation of antihypertensive and antihyperlipidemic effects of bamboo shoot angiotensin converting enzyme inhibitory peptide in vivo, J. Agric. Food Chem., 60(45), 11351 (2012). https://doi.org/10.1021/jf303471f
  31. S. M. An, S. I. Lee, S. W. Choi, S. W. Moon, and Y. C. Boo, p-Coumaric acid, a constituent of Sasa quelpaertensis Nakai, inhibits cellular melanogenesis stimulated by alpha-melanocyte stimulating hormone, Br. J. Dermatol., 159(2), 292 (2008). https://doi.org/10.1111/j.1365-2133.2008.08653.x
  32. K. Song and Y. C. Boo, UVB shielding effects of para-coumaric acid. J. Soc. Cosmet. Sci. Korea, 38(3), 263 (2012). https://doi.org/10.15230/SCSK.2012.38.3.263
  33. K. Song, S. M. An, M. Kim, J. S. Koh, and Y. C. Boo, Comparison of the antimelanogenic effects of p-coumaric acid and its methyl ester and their skin permeabilities, J. Dermatol. Sci., 63(1), 17 (2011). https://doi.org/10.1016/j.jdermsci.2011.03.012
  34. Y. K. Seo, S. J. Kim, Y. C. Boo, J. H. Baek, S. H. Lee, and J. S. Koh, Effects of p-coumaric acid on erythema and pigmentation of human skin exposed to ultraviolet radiation, Clin. Exp. Dermatol., 36(3), 260 (2011). https://doi.org/10.1111/j.1365-2230.2010.03983.x
  35. M. Brennan, H. Bhatti, K. C. Nerusu, N. Bhagavathula, S. Kang, G. J. Fisher, J. Varani, and J. J. Voorhees, Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin, Photochem. Photobiol., 78(1), 43 (2003). https://doi.org/10.1562/0031-8655(2003)078<0043:MMITMC>2.0.CO;2
  36. G. J. Fisher, S. Kang, J. Varani, Z. Bata-Csorgo, Y. Wan, S. Datta, and J. J. Voorhees, Mechanisms of photoaging and chronological skin aging, Arch. Dermatol., 138(11), 1462 (2002).
  37. G. J. Fisher, H. S. Talwar, J. Lin, P. Lin, F. McPhillips, Z. Wang, X. Li, Y. Wan, S. Kang, and J. J. Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin. Invest., 101(6), 1432 (1998). https://doi.org/10.1172/JCI2153
  38. P. Brenneisen, H. Sies, and K. Scharffetter-Kochanek, Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events, Ann. N. Y. Acad. Sci., 973, 31 (2002). https://doi.org/10.1111/j.1749-6632.2002.tb04602.x
  39. Y. J. Choi, H. S. Lim, J. S. Choi, S. Y. Shin, J. Y. Bae, S. W. Kang, I. J. Kang, and Y. H. Kang, Blockade of chronic high glucose-induced endothelial apoptosis by Sasa borealis bamboo extract, Exp. Biol. Med. (Maywood), 233(5), 580 (2008). https://doi.org/10.3181/0707-RM-205
  40. X. F. Qi, D. H. Kim, Y. S. Yoon, S. B. Song, Y. C. Teng, D. Q. Cai, and K. J. Lee, Bambusae caulis in Liquamen Suppresses the Expression of Thymus and Activation-Regulated Chemokine and Macrophage-Derived Chemokine in Human Keratinocytes due to Antioxidant Effect, Evid. Based Complement Alternat. Med., 2012, 617494 (2012).