Journal of Microbiology and Biotechnology

  • 제25권11호
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  • Pages.1782-1786
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  • 2015
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR

  • Yun, Yeo Hong (Department of Microbiology and Institute of Biodiversity, Dankook University) ;
  • Yoon, Seong Kwon (Department of Microbiology and Institute of Biodiversity, Dankook University) ;
  • Jung, Jae Sung (Department of Biology, Sunchon National University) ;
  • Kim, Seong Hwan (Department of Microbiology and Institute of Biodiversity, Dankook University)
  • 투고 : 2015.07.30
  • 심사 : 2015.09.01
  • 발행 : 2015.11.28
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초록

A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.

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참고문헌

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