The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Activation of Pathogenesis-related Genes by the Rhizobacterium, Bacillus sp. JS, Which Induces Systemic Resistance in Tobacco Plants

  • Kim, Ji-Seong (Graduate School of Life and Environmental Sciences, University of Tsukuba) ;
  • Lee, Jeongeun (Graduate School of Life and Environmental Sciences, University of Tsukuba) ;
  • Lee, Chan-Hui (Graduate School of Biotechnology, Kyung Hee University) ;
  • Woo, Su Young (Department of Environmental Horticulture, The University of Seoul) ;
  • Kang, Hoduck (Department of Biological and Environmental Science, Dongguk University) ;
  • Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul)
  • Received : 2014.11.25
  • Accepted : 2015.02.08
  • Published : 2015.06.01
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Abstract

Plant growth promoting rhizobacteria (PGPR) are known to confer disease resistance to plants. Bacillus sp. JS demonstrated antifungal activities against five fungal pathogens in in vitro assays. To verify whether the volatiles of Bacillus sp. JS confer disease resistance, tobacco leaves pre-treated with the volatiles were damaged by the fungal pathogen, Rhizoctonia solani and oomycete Phytophthora nicotianae. Pre-treated tobacco leaves had smaller lesion than the control plant leaves. In pathogenesis-related (PR) gene expression analysis, volatiles of Bacillus sp. JS caused the up-regulation of PR-2 encoding ${\beta}$-1,3-glucanase and acidic PR-3 encoding chitinase. Expression of acidic PR-4 encoding chitinase and acidic PR-9 encoding peroxidase increased gradually after exposure of the volatiles to Bacillus sp. JS. Basic PR-14 encoding lipid transfer protein was also increased. However, PR-1 genes, as markers of salicylic acid (SA) induced resistance, were not expressed. These results suggested that the volatiles of Bacillus sp. JS confer disease resistance against fungal and oomycete pathogens through PR genes expression.

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References

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