대한약침학회지 (Journal of Pharmacopuncture)

  • 제18권2호
  • /
  • Pages.60-66
  • /
  • 2015
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

Toxicity of Single-dose Intramuscular Injection of Samjeong Pharmacopuncture in Sprague-Dawley Rats

  • Kwon, Kang (Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University) ;
  • Kim, Chul-Yun (Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University) ;
  • Kim, Nam-Kwen (Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University) ;
  • Sun, Seung-Ho (Department of Acupuncture and Moxibustion, Sangji University College of Korean Medicine) ;
  • Seo, Hyung-Sik (Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University)
  • 투고 : 2015.03.20
  • 심사 : 2015.05.14
  • 발행 : 2015.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study was carried out in order to find both the single-dose intramuscular injection toxicity and the approximate lethal dose of samjeong pharmacopuncture (SP) in Sprague-Dawley (SD) rats. Methods: The SD rats in this study were divided into four groups, one control group (1.0 mL/animal, normal saline) and three experimental groups (0.25, 0.5, and 1.0 mL/animal, SP). All groups consisted of five male and five female rats. SP was injected as a single-dose intramuscularly at the thigh. After the injection, general symptoms and weight were observed for 14 days. After the observations had ended, hematologic and serum biochemical examinations, necropsy and a local tolerance test at the injection site were performed. The experiments were carried out at the Good Laboratory Practice firm, Biotoxtech Co. (Cheongwon, Chungbuk). Animal experiments were approved by the Ethics Committee (Approval Number: 130379). Results: No deaths occurred in any of the three experimental groups. The injection of SP had no effects on the general symptoms, body weights, results of the hematologic, and serum biochemical examinations, and necropsy findings. In local tolerance tests at the injection sites, mild inflammation was observed in the experimental group, but it did not appear to be a treatment related effect. Conclusion: Under the conditions of this test, the results from the injection of SP suggest that the approximate lethal dose of SP is above 1.0 mL/animal for both male and female SD rats. Therefore, the clinical use of SP is thought to be safe.

키워드

참고문헌

  1. Korean Pharmacopuncture Institute. Pharmacopuncturology: principles and clinical application. Seoul: Elsevier Korea LLC; 2012. p. 3-5.
  2. Son IH, Lee SM, Kim DH, Jeong HH, Cho SH, Lee EY, et al. A pilot study on single-dose toxicity testing of scolopendrid pharmacopuncture in sprague-dawley rats. J Pharmacopuncture. 2014;17(2):57-66. https://doi.org/10.3831/KPI.2014.17.017
  3. Yeo IH, Lee EY. Single-dose intramuscular toxicity of neutral natured blood stasis pharmacopuncture in sprague-dawley rats. J Pharmacopuncture. 2014;17(2):41-5. https://doi.org/10.3831/KPI.2014.17.015
  4. Jo SJ, Kim SC, Kim YJ, Kim EJ, Kim KS, Lee SD. Study of single-dose toxicity of guseonwangdo-go glucose intramuscular injection in sprague-dawley rats. J Pharmacopuncture. 2014;17(1):51-8. https://doi.org/10.3831/KPI.2014.17.007
  5. Lee HG, Kim SC, Jung DJ, Choi YM, Sin MS, Choi SW, et al. Single intramuscular-dose toxicity of water soluble carthmi-flos herbal acupuncture (WCF) in sprague-dawley rats. J Pharmacopuncture. 2014;17(1):27-34. https://doi.org/10.3831/KPI.2014.17.004
  6. Jung DJ, Kim SC, Lee HG, Choi YM, Sin MS, Choi SW, et al. Single intramuscular-dose toxicity of anti-inflammatory pharmacopuncture in rats. J Pharmacopuncture. 2013;16(4):22-9. https://doi.org/10.3831/KPI.2013.16.027
  7. Kim JK, Kim SH, Lee SM, Jeong HH, Park MY, Kim DW, et al. Study of single-dose toxicity of aconitum kusnezoffii reichb. pharmacopuncture in rats. J Pharmacopuncture. 2012;15(3):48-52. https://doi.org/10.3831/KPI.2012.15.007
  8. Yoon HC, Lee KH, Kwon KR. [Study of single dose toxic test of sweet bee venom in beagle dogs]. J Pharmacopuncture. 2010;13(4):43-61. Korean. https://doi.org/10.3831/KPI.2010.13.4.043
  9. Kim YJ, Lim CS, Kwon KR. [Study of single dose test of sweet bee venom in rats]. J Pharmacopuncture. 2009;12(4):5-31. Korean. https://doi.org/10.3831/KPI.2009.12.4.005
  10. Lee KH, Yu JS, Sun SH, Kwon KR, Lim CS. A 4-week, repeated, intravenous dose, toxicity test of mountain ginseng pharmacopuncture in sprague-dawley rats. J Pharmacopuncture. 2014;17(4):27-35. https://doi.org/10.3831/KPI.2014.17.034
  11. Kang HM, Lim CS, Kwon KR, Lee KH. Study of a 13-weeks, repeated, intramuscular dose, toxicity test of sweet bee venom in sprague-dawley rats. J Pharmacopuncture. 2014;17(2):73-9. https://doi.org/10.3831/KPI.2014.17.019
  12. Kang HM, Lim CS, Lee SB, Kim BW, Kwon KR, Lee KH. Study on a 4-week recovery test of sweet bee venom after a 13-week, repeated, intramuscular dose toxicity test in sprague-dawley rats. J Pharmacopuncture. 2014;17(2):18-26. https://doi.org/10.3831/KPI.2014.17.012
  13. Jo SJ, Kim SC, Kim YJ, Kim EJ, Kim KS, Lee SD. Study of single-dose toxicity of guseonwangdo-go glucose intramuscular injection in sprague-dawley rats. J Pharmacopuncture. 2014;17(1):51-8. https://doi.org/10.3831/KPI.2014.17.007
  14. Kwon HY, Kwon KR. [Study of four weeks repeated-dose toxic test of sweet bee venom in rats]. J Pharmacopuncture. 2011;14(1):5-24. Korean. https://doi.org/10.3831/KPI.2011.14.1.005
  15. Park JS, Lee KH, Kwon KR. [Study of four week repeated dose toxic test of sweet bee venom in beagle dogs. J Pharmacopuncture. 2010;13(4):5-41. Korean. https://doi.org/10.3831/KPI.2010.13.4.005
  16. Seo HS, Lee DJ. Testing of the safety and the effectiveness of using samjeong pharmacopuncture solution as eye drops. J Pharmacopuncture. 2012;15(1):23-8. https://doi.org/10.3831/KPI.2012.15.1.023