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Induction of Apoptosis by Gamma-Irradiated Apigenin in H1975 Human Non-Small Lung Cells

감마선 조사된 Apigenin의 H1975 인체 비소폐암세포에서의 Apoptosis 유발 효과

  • Park, Jae-Nam (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Eui-Baek (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jwa-Jin (Department of Anatomy, College of Medicine, Konyang University) ;
  • Jang, Beon-Su (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Sang-Hyun (Advance Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 박재남 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 변의백 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 김좌진 (건양대학교 의과대학) ;
  • 장범수 (한국원자력연구원 첨단방사선연구소 생명공학연구부) ;
  • 박상현 (한국원자력연구원 첨단방사선연구소 생명공학연구부)
  • Received : 2014.12.17
  • Accepted : 2015.04.21
  • Published : 2015.06.30

Abstract

The objective of this study was to evaluate the anticancer effects of gamma-irradiated apigenin against various human cancer cells. Structural changes were analyzed by high pressure liquid chromatography. Gamma-irradiated apigenin showed a new peak distinguished from the main peak of apigenin (non-irradiated). Cytotoxic effects in human normal cells (HS68) were not observed upon gamma-irradiated and non-irradiated apigenin treatment. However, gamma-irradiated apigenin treatment significantly increased cytotoxicity against non-small lung cancer cells. For apoptosis induction activity tested by Annexin V/PI staining, gamma-irradiated apigenin showed a stronger effect than non-irradiated apigenin, and the level of reactive oxygen species was apparently elevated by gamma-irradiated apigenin treatment. These results suggest that gamma irradiation could be an effective method for development of a new physiological compound from an original compound by inducing structural changes.

본 연구는 감마선 조사에 의해 유도된 apigenin 화합물(radiolysis products)이 인체유래 다양한 암세포에 처리했을 때의 항암 효과를 알아보기 위하여 실험을 진행하였다. Apigenin을 50 kGy로 조사할 경우 유도 화합물이 생성됨을 확인할 수 있었으며, radiolysis 화합물을 분획하여 인체유래 섬유육종세포(HS68)에 대한 독성을 평가한 결과 독성이 없는 것으로 나타났다. 다양한 암세포에 항암 활성을 평가한 결과 폐암(H1975)세포주의 경우 다른 암세포에 비해 효과가 높은 것으로 나타났다. Annexin V/PI 염색을 통해 감마선 조사된 apigenin 처리구에서 apoptosis의 발현을 확인할 수 있었으며, ROS(reactive oxygen species) 평가 결과에서도 농도 의존적으로 발현을 확인할 수 있었다. 본 연구는 방사선 구조 변환 연구를 통해 방사선을 이용한 새로운 신약 개발 가능성을 제시하는 기초자료로 활용될 수 있을 것으로 판단하였다.

Keywords

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