DOI QR코드

DOI QR Code

Whitening and Antioxidant Activities of Solvent Extracts from Hot-Air Dried Allium hookeri

열풍건조 삼채의 분획별 미백 및 항산화 활성에 관한 연구

  • Jeong, Su-Ji (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Kyoung-Hee (Department of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Department of Food and Nutrition, Chungnam National University)
  • Received : 2015.02.17
  • Accepted : 2015.05.14
  • Published : 2015.06.30

Abstract

The study analyzed the antioxidant and whitening activities of fractions from hot-air-dried Allium hookeri (HADAH). The total polyphenol contents in fractions from HADAH extract showed the highest value ($171.07{\pm}7.55mg/g$ GAE) in ethyl acetate fraction of roots. Antioxidant activities of leaf and root parts were significantly higher in the ethyl acetate fraction. HADAH showed higher whitening activity (n-hexane fraction of leaf; 52.22% melanin production) than arbutin (65.82% melanin production), which is known as a whitening ingredient. These results suggest that HADAH extracts can be used as antioxidant and whitening materials. Thus, study of HADAH extracts showing effective whitening and physiological activities can provide efficient data for industrial use of Allium hookeri.

열풍건조 삼채 잎 및 뿌리 부분의 메탄올 추출물과 순차적 분획물에 대하여 총 폴리페놀 함량과 항산화 활성을 측정하고, 미백 활성을 측정하여 삼채의 생리활성 효과와 화장품 원료 등의 기능성 소재로의 활용 가능성을 조사하였다. 열풍 건조 삼채 잎 및 뿌리 부분의 총 폴리페놀 함량은 뿌리의 ethyl acetate 분획물에서 $171.07{\pm}7.55mg/g$ GAE로 가장 높게 나타났다. 항산화 활성 측정 결과 잎과 뿌리 부분 모두 ethyl acetate 분획물에서 높은 활성을 나타내었다. 미백 활성 측정 결과 잎 부분의 n-hexane 분획물에서 tyrosinase 저해 활성은 2 mg/mL의 농도에서 $53.51{\pm}2.55%$로 높은 활성을 나타내었으며 melanin 생성 억제 효과도 52.22%의 멜라닌 생성으로 양성대조군으로 쓰인 알부틴의 65.82%보다도 더 높은 멜라닌 생성 억제 효과를 나타내었다. 이상의 연구 결과로 삼채의 메탄올 추출물 및 용매 분획물은 항산화 활성을 가지고 있고, tyrosinase 저해 활성 및 melanin 생성 억제 효과를 나타내어 건강기능식품 및 화장품 관련 제품으로서의 개발 가능성이 있음을 확인하였다.

Keywords

References

  1. Kim EJ, Ahn SY, Nam GW, Lee HK, Moon SJ, Kim YM, Oh MS, Kim NS, Chang IS, Park SK. 2006. The anti-aging effect of the cosmetic products containing the needles of red pine on human skin. Kor J Herbology 21: 25-31.
  2. Papa S, Skulachev VP. 1997. Reactive oxygen species, mitochondria, apoptosis and aging. Mol Cell Biochem 174:305-319. https://doi.org/10.1023/A:1006873518427
  3. Fantone JC, Ward PA. 1982. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 107: 395-418.
  4. Bae SM, Kim JH, Cho CW, Jeong TJ, Ha JU, Lee SC. 2001. Effect of microwave treatment on the antioxidant activity of rice processed by-products. J Korean Soc Food Sci Nutr 30: 1026-1032.
  5. Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16: 33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  6. Morrissey PA, O'Brien NM. 1998. Dietary antioxidants in health and disease. Int Dairy J 8: 463-472. https://doi.org/10.1016/S0958-6946(98)00070-3
  7. Ayam VS. 2011. Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur. Afr J Food Agric Nutr Dev 11: 5389-5412.
  8. You BR, Kim E, Jang JY, Choi HJ, Kim HJ. 2013. Quality characteristics of kimchi with Allium hookeri root powder added. Korean J Food Preserv 20: 863-870. https://doi.org/10.11002/kjfp.2013.20.6.863
  9. Park JY, Yoon KY. 2014. Comparison of the nutrient composition and quality of the root of Allium hookeri grown in Korea and Myanmar. Korean J Food Sci Technol 46:544-548. https://doi.org/10.9721/KJFST.2014.46.5.544
  10. Rhyu DY, Park SH. 2013. Characterization of alkyl thiosulfinate in Allium hookeri root using HPLC-ESI-MS. J Korean Soc Appl Biol Chem 56: 457-459. https://doi.org/10.1007/s13765-013-3069-x
  11. Hsu CC, Huang CN, Hung YC, Yin MC. 2004. Five cysteine-containing compounds have antioxidative activity in Balb/cA mice. J Nutr 134: 149-152. https://doi.org/10.1093/jn/134.1.149
  12. Welch C, Wuarin L, Sidell N. 1992. Antiproliferative effect of the garlic compound S-allyl cysteine on human neuroblastoma cells in vitro. Cancer Lett 63: 211-219. https://doi.org/10.1016/0304-3835(92)90263-U
  13. Banerjee SK, Maulik SK. 2002. Effect of garlic on cardiovascular disorders: a review. Nutr J 1: 4. https://doi.org/10.1186/1475-2891-1-4
  14. Keusgen M. 2002. Health and alliums. In Allium Crop Science-Recent Advances. CABI, Wallingford, UK. p 357-378.
  15. Bae GC, Bae DY. 2012. The anti-inflammatory effects of ethanol extract of Allium hookeri cultivated in South Korea. Kor J Herbology 27: 55-61.
  16. Kim KH, Kim HJ, Byun MW, Yook HS. 2012. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J Korean Soc Food Sci Nutr 41: 577-583. https://doi.org/10.3746/jkfn.2012.41.5.577
  17. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
  18. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  19. Fellegrini N, Ke R, Yang M, Rice-Evans C. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline- 6-sulfonic acid radical cation decolorization assay. Method Enzymol 299: 379-389.
  20. Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 239: 70-76. https://doi.org/10.1006/abio.1996.0292
  21. Flurkey WH. 1991. Identification of tyrosinase in mushrooms by isoelectric focusing. J Food Sci 56: 93-95. https://doi.org/10.1111/j.1365-2621.1991.tb07983.x
  22. Park YJ, Yoon MY, Lim HW, Lee JY, Kim CJ, Sim SS. 2004. Effect of hot-water extracts from Laminaria japonicus on melanin production in B16 melanoma cells. Yakhak Hoeji 48: 374-378.
  23. Hyon JS, Kang SM, Senevirathne M, Koh WJ, Yang TS, Oh MC, Oh CK, Jeon YJ, Kim SH. 2010. Antioxidative activities of extracts from dried Citrus sunki and C. unshiu peels. J Korean Soc Food Sci Nutr 39: 1-7. https://doi.org/10.3746/jkfn.2010.39.1.001
  24. Lee SE, Lee SW, Bang JK, Yu YJ, Seong NS. 2004. Antioxidant activities of leaf, stem and root of Panax ginseng C. A. Meyer. Korean J Medicinal Crop Sci 12: 237-242.
  25. Lee HJ, Lee BJ, Lee DS, Seo Y. 2003. DPPH radical scavenging effect and in vitro lipid peroxidation inhibition by Portulaca oleracea. Korean J Biotechnol Bioeng 18: 165-169.
  26. Wang LF, Zhang HY. 2003. A theoretical investigation on DPPH radical-scavenging mechanism of edaravone. Bioorg Med Chem Lett 13: 3789-3792. https://doi.org/10.1016/j.bmcl.2003.07.016
  27. Muller L, Theile K, Bohm V. 2010. In vitro antioxidant activity of tocopherols and tocotrienols and comparison of vitamin E concentration and lipophilic antioxidant capacity in human plasma. Mol Nutr Food Res 54: 731-742. https://doi.org/10.1002/mnfr.200900399
  28. Joo SY. 2013. Antioxidant activities of medicinal plant extracts. J Korean Soc Food Nutr 41: 512-519.
  29. Song JC, Park NK, Hur HS, Bang MH, Baek NI. 2000. Examination and isolation of natural antioxidants from Korean medicinal plants. Korean J Medicinal Crop Sci 8:94-101.
  30. Kim JE, Joo S, Seo JH, Lee SP. 2009. Antioxidant and ${\alpha}$- glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 38: 989-995. https://doi.org/10.3746/jkfn.2009.38.8.989
  31. Choi JS, Kim HY, Seo WT, Lee JH, Cho KM. 2012. Roasting enhances antioxidant effect of bitter melon (Momordica charantia L.) increasing in flavan-3-ol and phenolic acid contents. Food Sci Biotechnol 21: 19-26. https://doi.org/10.1007/s10068-012-0003-7
  32. Nicoli MC, Anese M, Manzocco L, Lerici CR. 1997. Antioxidant properties of coffee brews in relation to the roasting degree. LWT - Food Sci Technol 30: 292-297. https://doi.org/10.1006/fstl.1996.0181
  33. Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290-4302. https://doi.org/10.1021/jf0502698
  34. Kwak JH, Seo UK, Han YH. 2001. Inhibitory effect of mugwort extracts on tyrosinase activity. Korean J Biotechnol Bioeng 16: 220-223.
  35. Bennett DC, Cooper PJ, Hart IR. 1987. A line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth. Int J Cancer 39: 414-418. https://doi.org/10.1002/ijc.2910390324
  36. Yang YM, Son YO, Lee SA, Jeon YM, Lee JC. 2011. Quercetin inhibits ${\alpha}$-MSH-stimulated melanogenesis in B16F10 melanoma cells. Phytother Res 25: 1166-1673. https://doi.org/10.1002/ptr.3417
  37. Arung ET, Furuta S, Ishikawa H, Tanaka H, Shimizu K, Kondo R. 2011. Melanin biosynthesis inhibitory and antioxidant activities of quercetin-3'-O-beta-D-glucoside isolated from Allium cepa. Z Naturforsch C 66: 209-214. https://doi.org/10.5560/ZNC.2011.66c0209
  38. Seo EJ, Hong ES, Choi MH, Kim KS, Lee SJ. 2012. The antioxidant and skin whitening effect of Artemisia iwayomogi extracts. Korean J Food Sci Technol 44: 89-93. https://doi.org/10.9721/KJFST.2012.44.1.089
  39. Lee KM, Lee EC, Cho SC, Moon SS. 2008. The antimelanogenic effects of compounds extracted from bamboo inner film. J Soc Cosmet Scientists Korea 34: 287-301.

Cited by

  1. Physicochemical and antioxidant properties in Allium hookeri by hot air-and freeze-drying methods vol.23, pp.1, 2016, https://doi.org/10.11002/kjfp.2016.23.1.57
  2. Antioxidant and anti-inflammatory activities of Opuntia ficus-indica and Opuntia humifusa fruits ethanol extracts vol.25, pp.5, 2018, https://doi.org/10.11002/kjfp.2018.25.5.586
  3. 발아녹미와 노루궁뎅이 버섯 균사체를 이용한 생물전환 약용 신소재의 항산화 활성 및 NO 생성 억제 효능 vol.25, pp.5, 2015, https://doi.org/10.7783/kjmcs.2017.25.5.305
  4. B16F10 melanoma 세포에서 미성숙 사과 과피 열수추출물의 tyrosinase 활성과 melanin 생 성에 미치는 영향 vol.28, pp.8, 2018, https://doi.org/10.5352/jls.2018.28.8.900
  5. 자외선 B에 유도된 사람유래 HaCaT cells에서의 오미자 종자 분획물의 항산화 및 항노화 효과 vol.29, pp.10, 2019, https://doi.org/10.5352/jls.2019.29.10.1071
  6. Spirostane-type steroidal saponin from Allium hookeri roots with mushroom tyrosinase inhibitory activity vol.23, pp.4, 2019, https://doi.org/10.6564/jkmrs.2019.23.4.087
  7. 애플망고 잎의 추출물의 항산화 및 대식세포(RAW 264.7)에서 iNOS, COX-2 발현 저해 효과 vol.30, pp.9, 2020, https://doi.org/10.5352/jls.2020.30.9.783
  8. 편백나무(Chamaecyparis obtusa) 추출물의 항산화, 미백효과에 관한 연구 vol.37, pp.6, 2020, https://doi.org/10.12925/jkocs.2020.37.6.1496
  9. Evaluation of Antioxidant and Antimicrobial Activity of Buah Merah (Pandenus conoideus) by Solvent Fractionation vol.50, pp.8, 2015, https://doi.org/10.3746/jkfn.2021.50.8.792