DOI QR코드

DOI QR Code

A study on comparison and analysis of chlorophyll sensor with aceton extraction for chlorophyll measurement in the Nakdong River

낙동강에서 클로로필(Chlorophyll) 측정을 위한 클로로필 센서와 아세톤 추출법의 비교분석에 관한 연구

  • Received : 2015.04.02
  • Accepted : 2015.05.15
  • Published : 2015.06.15

Abstract

Concerns about water quality in the Nakdong River have been raised because the Nakdong River will change from a lotic environment to a lentic environmental due to the installation of eight weirs to be constructed as part of the Four Major Rivers Restoration Project. The rapid urbanization and industrialization of the middle and the lower reaches of Nakdong River causes the indiscreet discharge of uncleanly living sewage and industrial wastewater. And the water quality of lower reaches of Nakdong River is getting seriously worse. Owing to the water shortage of Nakdong River and the closing of reaches because of the estuary dyke in the dry season, the velocity of a moving fluid is almost accumulated under 0.03m/sec. Then a pollutant is piled up on the bottom of the river. Polluted sediment is formed and nutrition level of water is increased more and more. The eutrophication state propagated to dark brown or green from eutrophication often comes out. Therefore in this study, we measured Chl. a of chlorophyll sensor (YSI6600V2) and aceton extraction through field observation in the Nakdong River and Samrangjin. And we evaluated the reliability of chlorophyll sensor. In correlation analysis between chlorophyll sensor and aceton extraction, it shows high relation in general. And it also shows high relation among the chlorophyll sensor and aceton extraction of the dominant diatom (Skeletonema costatum), Dinophyta (Prorocentrum minimum) in the Nakdong River estuary by laboratory analysis results.

Keywords

References

  1. Eullaffroy, P. and Vernet, G. (2003) The F684/F735 chlorophyll fluorescence ratio; a potential tool for rapid detection and determination of herbicide phytotoxicity in algae. Water Res., 37, 1983-1990. https://doi.org/10.1016/S0043-1354(02)00621-8
  2. Hallegraeff, G. M. (1981) Seasonal study of phytoplankton pigments and species at a coastal station off Sydney: importance of diatoms and the nanoplankton. Mar. Biol., 61, 107-118. https://doi.org/10.1007/BF00386650
  3. Jan kohler, (1993) Growth production and losses phytoplankton in the lowland River Spree. I. Population dynamics, J. Plankton Res 15(3), 337-349.
  4. Jeffrey, S. W. and LeRoi, J. M. (1997) Simple procedure for growing SCOR reference microalgal cultures. In: Jeffrey, S. W., Mantoura, R. F. C. and Wright, S. W. (Eds.), Phytoplankton pigments in oceanography. UNESCO Publ., 181-205.
  5. Lichenthaler, H. H. (1988) Application of chlorophyll fluorescence. Kluwer Academic Press, Dordrecht.
  6. Lorenzen, C. J. (1967) Determination of chlorophyll and pheopigments: Spectrophotometric equations. Limnol. Oceanogr., 12, 343-346. https://doi.org/10.4319/lo.1967.12.2.0343
  7. Marker, A. F. H. (1972) The use of acetone and methanol in the estimation of chlorophyll in the presence of phaeophytin. Freshwat. Biol., 2, 361-385. https://doi.org/10.1111/j.1365-2427.1972.tb00377.x
  8. Margalef, R. (1983) Ecologia del fitoplancon in Limnologia. Omega, Barcelona. 247-330.
  9. Ministry of Land, Transport and Maritime Affairs (2010) Maritime environment pollutant testing method. pp. 495.
  10. Nusch, E. A. (1980) Comparison of methods for chlorophyll and phaeopigment determination. Arch. Hydrobiol. Beih. Ergebn. Limnol., 14, 14-36.
  11. Parsons, T. R. and Strickland, J. D. H. (1963) Discussion of spectrophotometric determination of marine plant pigments, with revised equations for ascertaining chlorophylls and carotenoids. J. Mar. Res., 21, 155-163.
  12. SCOR-UNESCO, (1966) Determination of photosynthetic pigments in seawater. In: Monographs on Oceanographic Methodology I. Unesco (Ed.), Paris, 11-18.
  13. Shin, J. H. (2013) A study on the Bayesian multilevel regression model for the algal bloom characteristics in the downstream of Nakdong River, Master's Thesis, Ewha Womans University, Seoul, Republic of Korea, pp. 53-60.
  14. Shoaf, W. T. and Lium, B. W. (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulphoxide. Limnol. Oceanogr., 21, 926-928. https://doi.org/10.4319/lo.1976.21.6.0926
  15. Suzuki, R. and Ishimaru, T. (1990) An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide. J. Oceanogr. Soc. Jpn., 46, 190-194. https://doi.org/10.1007/BF02125580
  16. Yentch, C. S. and Phinney, D. A. (1985) Spectral fluorescence: a taxonomic tool for studying the structure of phytoplankton populations. J. Plankton Res., 7, 617-632. https://doi.org/10.1093/plankt/7.5.617

Cited by

  1. Toxicity Evaluation of Individual and Mixtures of Nanoparticles Based on Algal Chlorophyll Content and Cell Count vol.11, pp.1, 2018, https://doi.org/10.3390/ma11010121
  2. Analysis of Phytoplankton Community Change According to Continuous Observation Pattern of Chlorophyll-a Concentration vol.21, pp.2, 2018, https://doi.org/10.7846/JKOSMEE.2018.21.2.107
  3. Comparative Effects of Particle Sizes of Cobalt Nanoparticles to Nine Biological Activities vol.21, pp.18, 2020, https://doi.org/10.3390/ijms21186767
  4. Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of Pu- and Pm-lux Recombinant P. putida mt-2 Strains vol.11, pp.11, 2015, https://doi.org/10.3390/nano11112822