수산해양교육연구 (Journal of Fisheries and Marine Sciences Education)

  • 제27권3호
  • /
  • Pages.879-889
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  • 2015
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  • 1229-8999(pISSN)
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  • 2288-2049(eISSN)
한국수산해양교육학회 (The Korean Society for Fisheries and Marine Sciences Education)
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2014년 제주도 양식 넙치, Paralichthys olivaceus에 대한 VHS 및 RSIVD 모니터링

Monitoring of VHS and RSIVD in cultured Paralichthys olivaceus of Jeju in 2014

  • 박현경 (제주대학교 해양의생명과학부) ;
  • 김승민 (제주대학교 해양의생명과학부) ;
  • 이다원 (제주대학교 해양의생명과학부) ;
  • 전려진 (제주대학교 수산백신연구센터) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • PARK, Hyun-Kyung (Faculty of Marine Biomedical Science, Jeju National University) ;
  • KIM, Seung-Min (Faculty of Marine Biomedical Science, Jeju National University) ;
  • LEE, Da-Won (Faculty of Marine Biomedical Science, Jeju National University) ;
  • JUN, Lyu-Jin (Fish Vaccine Resarch Center, Jeju National University) ;
  • JEONG, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University)
  • 투고 : 2015.06.08
  • 심사 : 2015.06.19
  • 발행 : 2015.06.30
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초록

The outbreak of viral diseases caused by viral haemorrhagic septicaemia virus (VHSV) and red seabream iridovirus (RSIV) have been reported in cultured olive flounder, Paralichthys olivaceus. VHSV has been a serious viral disease that infects the olive flounders in South Korea. Clinical signs of VHSV infection are skin darkening, abdominal distension and haemorrhages. Outbreaks of fish iridoviral disease was first reported from red seabream, Pagrus major farms in Japan. Recently, iridovirus infection have occurred frequently from olive flounder farms in South Korea. In this study, disease surveillance was performed to monitor the prevalence of VHSV and RSIV in olive flounder in 2014. The samples were collected from 60 different olive flounder farms in Jeju from April, May, September, November and December in 2014. RT-PCR (VHSV) or PCR (RSIV) results showed that VHSV were detected in 5 farms, but RSIV has not been detected in any farms. The migration of olive flounder was restricted for the quarantine in 5 farms of VHS outbreak. The nucleocapsid protein (N) gene and glycoprotein (G) gene sequences of the 5 Korean VHSV isolates were successfully amplified and sequenced. Phylogenetic analysis was performed using the VHSV sequences reported here together comparison with the nucleotide sequences available from the GenBank database. Phylogenetic analysis indicated that most of Korea VHSV belong to the genotype IVa and closely related to the strains from Japan and China.

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참고문헌

  1. Bourhy, Herve.Kissi, Bachir.Lafon, Monique.Sacramento, Debora & Tordo, Noel(1992). Antigenic and molecular characterization of bat rabies virus in Europe, Journal of Clinical Microbiology 30(9), 2419-2426.
  2. Cho, Mi-Young.Kim, Myoung-Sug.Kwon, Mun-Gyeong.Jee, Bo-Young.Choi, Hye-Sung.Choi, Dong-Lim.Park, Gyeong-Hyun.Lee, Chang-Hoon.Kim, Jin-Do.Lee, Joo-Seok.Oh, Yun-Kyeong.Lee, Deok-Chan.Park, Shin-Hoo & Park, Myoung-Ae(2007). Epidemiological study of bacterial diseases of cultured olive flounder, Paralichthys olivaceus from 2005 to 2006 in Korea, Journal of Fish Pathology 20(1), 61-70.
  3. Cho, Mi-Young.Park, Gyeong-Hyun.Jee, Bo-Young & Kim, Jin-Woo(2010). Statistical data on fish virus of cultured olive flounder, Paralichthys olivaceus from 2005 to 2007, Journal of Fish Pathology 23(2), 155-162.
  4. Dale, Ole-Bendik Orpetveit,.Irene.Lyngstad, Trude-Marie.Kahns, Soren.Skall, Helle-Frank.Olesen, Niels-Jorgen & Dannevig, Birgit-Helene (2009). Outbreak of viral haemorrhagic septicaemia (VHS) in seawater farmed rainbow trout in Norway caused by VHS virus Genotype III, Diseases of Aquatic Organisms 85(2), 93-103. https://doi.org/10.3354/dao02065
  5. Dixon, P. F..Feist, S..Kehoe, E..Parry, L..Stone, D. M. & Way, K.(1997). Isolation of viral haemorrhagic septicaemia virus from Atlantic herring Clupea harengus from the English Channel, Diseases of Aquatic Organisms 30, 81-89. https://doi.org/10.3354/dao030081
  6. Do, Jeong-Wan.Moon, Chang-Hoon.Kim, Hyo-Jeong.Ko, Myoung-Seok.Kim, Sung-Bum.Son, Ji-Hee.Kim, Jong-Soo.An, Eun-Jeong.Kim, Min-Kyoung.Lee, Seung-Koo.Han, Myung-Shin.Cha, Seung-Ju.Park, Mi-Seon.Park, Myoung-Ae.Kim, Yi-Cheng.Kim, Jin-Woo & Park, Jeong Woo(2004). Complete genomic DNA sequence of rock bream iridovirus, Virology 325(2), 351-363. https://doi.org/10.1016/j.virol.2004.05.008
  7. Do, Jeong-Wan.Cha, Seung-Ju.Kim, Jong-Su.An, Eun-Jeong.Lee, Nam-Sil.Choi, Hee-Jung.Lee, Chan-Hwei.Park, Mi-Seon.Kim, Jin-Woo.Kim, Yi-Cheong & Park, Jeong-Woo(2005). Phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders Paralichthys olivaceus in Korea, Diseases of Aquatic Organisms 64(3), 193-200. https://doi.org/10.3354/dao064193
  8. Do, Jeong-Wan.Cha, Seung-Ju.Kim, Jong-Su.An, Eun-Jeong.Park, Mi-Seon.Kim, Jin-Woo.Kim, Yi-Cheong.Park, Myoung-Ae & Park, Jeong Woo(2005). Sequence variation in the gene encoding the major capsid protein of Korean fish iridoviruses, Archives of Virology 150(2), 351-359. https://doi.org/10.1007/s00705-004-0424-6
  9. Einer-Jensen, Katja.Ahrens, Peter.Forsberg, Roald & Lorenzen, Niels(2004). Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus, Journal of General Virology 85(5), 1167-1179. https://doi.org/10.1099/vir.0.79820-0
  10. He, Jian-G.Deng, Min.Weng, Shao-P.Li, Zheng.Zhou, Song-Y.Long, Qin-X.Wang, Xun-Z & Chan, Siu-Ming(2001). Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus, Virology 291, 126-139. https://doi.org/10.1006/viro.2001.1208
  11. Inouye, K..Yamano, K..Maeno, Y..Nakajima, K..Matsuoka, M..Wada, Y. & Sorimachi, M.(1992). Iridovirus infection of cultured red sea bream, Pagrus major, Fish Pathology 27, 19-27. https://doi.org/10.3147/jsfp.27.19
  12. Kim, Su-Mi.Lee, Jae-Il,.Hong, Mi-Ju.Park, Heon-Sik & Park, Soo-Il(2003). Genetic relationship of the VHSV (viral haemorrhagic septicaemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea, Journal of Fish Pathology 16(1), 1-12.
  13. Kim, Wi-Sik.Kim, Seok-Ryel.Kim, Du-Woon.Kim, Jong-Oh.Park, Myoung-Ae.Kitamura, Shin-Ichi.Kim, Heung-Yun.Kim, Do-Hyung.Han, Hyun-Ja.Jung, Sung-Ju & Oh, Myung-Joo (2009). An outbreak of VHSV (viral haemorrhagic septicaemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea, Aquaculture 296(1), 165-168. https://doi.org/10.1016/j.aquaculture.2009.07.019
  14. Lee, Wol-La.Kim, Seok-Ryel.Yun, Hyun-Mi.Kitamura, Shin-Ichi.Jung, Sung-Ju.Oh, Myung-Joo (2007). Detection of red sea bream iridovirus (RSIV) from marine fish in the Southern coastal area and East China Sea, Journal of Fish Pathology 20(3), 211-220.
  15. Lumsden, J. S..Morrison, B..Yason, C..Russell, S..Young, K..Yazdanpanah, A..Hurber, P..Al-Hussinee, L. Stone, D.. & Way, K.(2007). Mortality event in freshwater drum Aplodinotus grunniens from Lake Ontario, Canada, associated with viral haemorrhagic septicaemia virus, Type IV, Diseases of Aquatic Organisms 76(2), 99-111. https://doi.org/10.3354/dao076099
  16. Matsuoka, S..Inouye, K. & Nakajima, K.(1996). Cultured fish species infected by red sea bream iridoviral disease from 1991 to 1995, Journal of Fish Pathology 31(4), 233-234. https://doi.org/10.3147/jsfp.31.233
  17. MEST(Ministry of Education and Science Technology). 2010. Atlas of Fish Diseases.
  18. Mortensen, Helle-Frank.Heuer, Ole-Eske.Lorenzen, Niels․Otte, Lars & Olesen, Niels-Jorgen (1999). Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea, Virus Research 63(1), 95-106. https://doi.org/10.1016/S0168-1702(99)00062-3
  19. Nishizawa, T..Iida, H..Takano, R..Isshiki, T..Nakajima, K. & Muroga, K.(2002). Genetic relatedness among Japanese, American and European isolates of viral haemorrhagic septicaemia virus (VHSV) based on partial G and P genes, Diseases of Aquatic Organisms 48(2), 143-148. https://doi.org/10.3354/dao048143
  20. OIE.(2013). World Organization for Animal Health.
  21. Sano, Motohiko.Ito, Takafumi.Matsuyama, Tomomasa.Nakayasu, Chihaya & Kurita, Jun(2009). Effect of water temperature shifting on mortality of Japanese flounder Paralichthys olivaceus experimentally infected with viral haemorrhagic septicaemia virus, Aquaculture 286(3), 254-258. https://doi.org/10.1016/j.aquaculture.2008.09.031
  22. Shi, Cheng-Yin.Wang, Yin-Geng.Yang, Shao-Li.Huang, Jie & Wang, Qing-Yin(2004). The first report of an iridovirus-like agent infection in farmed turbot, Scophthalmus maximus, in China, Aquaculture 236(1), 11-25. https://doi.org/10.1016/j.aquaculture.2003.11.007
  23. Snow, Michael.Cunningham, Carey-O.Melvin, William-T & Kurath, Gael(1999). Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment, Virus Research 63(1), 35-44. https://doi.org/10.1016/S0168-1702(99)00056-8
  24. Takano, R..Nishizawa, T..Arimoto, M. & Muroga, K.(2000). Isolation of viral haemorrhagic septicaemia (VHSV) from wild Japanese flounder, Paralichthys olivaceus, Bulletin European Association of Fish Pathologists 20(5), 186-193.
  25. Tamura, K..Dudley, J..Nei, M. & Kumar, S.(2007). Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, Molecular Biology and Evolution 24(8), 1596-1599. https://doi.org/10.1093/molbev/msm092
  26. Thompson, J. D..Higgins, D. G. & Gibson, T. J.(1994). Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Research 22(22), 4673-4680. https://doi.org/10.1093/nar/22.22.4673