Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Development of PCR-base Diagnostic System for the Detection of Andean potato latent virus

Andean potato latent virus 검출을 위한 PCR 기반 진단시스템 개발

  • Lee, Jin-Young (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency) ;
  • Kim, Jin-Ho (Department of Chemistry, Dankook University) ;
  • Kim, Eunsil (Department of Crop Science & Biotechnology, Dankook University) ;
  • Lee, Siwon (Environmental Infrastructure Research Department, National Institute of Environmental Research)
  • 이진영 (농림축산검역본부 인천공항지역본부) ;
  • 김진호 (단국대학교 화학과) ;
  • 김은실 (단국대학교 식량생명공학과) ;
  • 이시원 (국립환경과학원 환경기반연구부)
  • Received : 2015.03.07
  • Accepted : 2015.06.17
  • Published : 2015.06.30
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Abstract

Andean potato latent virus (APLV) is a phytopathogenic virus that belongs to the Group IV (+) sense ssRNA viruses of the genus Tymovirus. It mainly infects potatoes and is specified as a controlled quarantine virus in Korea. In this study, two primer sets of RT-PCR and nested PCR [set 2 ($404{\rightarrow}259bp$) and set 23 ($501{\rightarrow}349bp$)], were selected, which can rapidly and accurately diagnose APLV in quarantine sites. In addition, a modified-positive control plasmid is development, can possible verification of laboratory contamination in diagnosis of APLV detection. The PCR-base system developed in this study is expected to diagnose APLV and contribute to the plant quarantine in Korea.

Andean potato latent virus (APLV)는 Group IV (+) sense ssRNA viruses, Tymovirus속으로 분류되는 식물 병원성 바이러스로, 주로 감자에 감염되며 국내 관리급 검역바이러스로 지정되어 있다. 본 연구에서는 검역현장에서 APLV를 신속 정확하게 진단할 수 있는 2개의 RT-PCR 프라이머 조합[조합 2 (404 bp)와 23 (501 bp)]과 각각의 nested PCR 조합($404{\rightarrow}259bp$ 및 $501{\rightarrow}349bp$)을 선발하였다. 또한, 진단에 양성대조군으로 활용할 수 있는 유전자변형 양성대조구를 개발하여 실험실 오염에 대한 검증이 가능하도록 하였다. 본 연구에서 개발한 PCR 기반 진단시스템은 향후 검역 현장에서 APLV를 검출하여 우리나라 식물검역에 기여할 것이라고 기대된다.

Keywords

References

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