Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum

  • Lee, Jin-Young (Department of Biological Sciences, College of Natural Sciences, Chonnam National University) ;
  • Choy, Hyon E. (Department of Microbiology, Chonnam National University Medical School) ;
  • Lee, Jin-Ho (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Kim, Geun-Joong (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
  • Received : 2014.08.18
  • Accepted : 2014.10.21
  • Published : 2015.04.28
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Abstract

Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli. Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ${\Delta}parA$ and ${\Delta}ncgl1366$, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.

Keywords

References

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