Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of 4-Hexylresorcinol Treatment on Melanosis Inhibition and Residual Levels in Korean Shrimp

4-Hexylresorcinol 침지액 농도와 침지시간에 따라 새우의 갈변도 및 4-Hexylresorcinol 잔류량에 미치는 영향

  • Kim, Young-Hyun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Jae-Min (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Sol (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Gang, Seong-Ran (Food Safety Research Institute Analytical Laboratory, S-Food Guardians Co., Ltd.) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 김영현 (강원대학교 식품생명공학과) ;
  • 김재민 (강원대학교 식품생명공학과) ;
  • 이진솔 (강원대학교 식품생명공학과) ;
  • 강성란 ((주)에스푸드가디언스) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2015.03.04
  • Accepted : 2015.04.17
  • Published : 2015.07.31
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Abstract

4-Hexylresorcinol (4-HR) has been used for prevention of melanosis in shrimp. Recently, 4-HR in EU and other countries was authorized with maximum residue levels of 1~2 mg/kg but remains unauthorized in Korea. Therefore, the aim of this study was to investigate the effect of 4-HR on melanosis inhibition and residual levels in Korean shrimp according to various concentrations and soaking times. We previously reported that the HPLC-FLD method can be used for detection of 4-HR at maximum levels of 1~2 mg/kg in shrimp. Our results show that residual levels of 4-HR in peeled and unpeeled shrimps were in the range of 0.86 to 13.46 mg/kg and 0.02 to 2.33 mg/kg, respectively. In addition, 4-HR treatment at concentrations of 10 and 20 mg/L for 72 h were effective for inhibition of melanosis in peeled shrimp compared with unpeeled shrimp. These results provide basic data for the establishment of guidelines and regulation related to 4-HR.

껍질을 제거하지 않은 새우의 경우 10일 동안 저장하는 과정에서 갈변이 심각하게 진행된 것을 확인할 수 있었다. 갈변은 두흉부, 등껍질 그리고 꼬리 순서로 진행되었으며, 4-hexylresorcinol 침지액에 의해 갈변이 억제될 경우 꼬리, 등껍질 그리고 두흉부 순서로 억제되었다. 껍질을 제거하지 않은 새우의 경우 10, 20 mg/L 4-hexylresorcinol 침지액 농도에서 72시간 침지한 새우로부터 각각 1.36, 2.33 mg/kg의 잔류농도를 확인하였으며, 해외에서 규정하고 있는 최대잔류수준 이상의 4-hexylresorcinol이 검출되어 갈변억제 효과는 있었지만 사용방법으로 부적합한 것으로 나타났다. 껍질을 제거한 새우의 경우에는 가식부위가 직접 침지액에 침지되기 때문에 대부분의 새우에서 1 mg/kg 이상의 4-hexylresorcinol 잔류량이 확인되었으며, 껍질을 제거한 새우를 20 mg/L 4-hexylresorcinol 침지액에 72시간 동안 침지한 처리군에서 13.46 mg/kg으로 가장 높은 4-hexylresorcinol 잔류량이 확인되었다. 이러한 결과는 캐나다 및 중국뿐만 아니라 유럽에서 규정하고 있는 최대잔류수준을 초과함에 따라서 새우의 침지 조건으로 새우 껍질을 제거한 뒤 침지하는 방법은 적합하지 않은 것으로 판단되며, 갈변억제 효과는 20 mg/L 4-hexylresorcinol 침지액에서 24시간 동안 침지한 처리군에서 가장 높은 억제 효과를 나타내었다. 본 연구 결과 4-hexylresorcinol 침지액 농도와 침지 시간에 따라 4-hexylresorcinol 잔류량 및 갈변억제 효과에 높은 상관관계를 나타내었으며, 규정에 제시되어 있는 최대잔류허용량을 초과하지 않고 효율적인 갈변억제 효과를 얻기 위한 방법으로는 껍질을 제거하지 않은 새우에서 20 mg/L 4-hexylresorcinol 침지액에서 24시간 동안 침지처리하는 방법이 가장 적절한 것으로 확인되었다.

Keywords

References

  1. Manheem K, Benjakul S, Kijroongrojana K, Visessanguan W. 2012. The effect of heating conditions on polyphenol oxidase, proteases and melanosis in pre-cooked Pacific white shrimp during refrigerated storage. Food Chem 131: 1370- 1375. https://doi.org/10.1016/j.foodchem.2011.10.001
  2. Encarnacion AB, Fagutao F, Jintasataporn O, Worawattanamateekul W, Hirono I, Ohshima T. 2012. Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei. Food Res Int 45: 232-237. https://doi.org/10.1016/j.foodres.2011.10.030
  3. Jeong JW, Park KJ, Sung JM, Kim JH, Kwon KH. 2006. Comparison of quality of peeled lotus roots stored in various immersion liquids during storage. Korean J Food Sci Technol 38: 526-533.
  4. Whitaker JR. 1993. Principles of enzymology for the food sciences. In Food Science and Technology. Marcel Dekker, Inc., CRC Press, New York, NY, USA. Vol 61, p 241-270.
  5. Montero P, Avalos A, Perez-Mateos M. 2001. Characterization of polyphenoloxidase of prawns (Penaeus japonicus). Alternatives to inhibition: additives and high-pressure treatment. Food Chem 75: 317-324. https://doi.org/10.1016/S0308-8146(01)00206-0
  6. Simpson BK, Marshall MR, Otwell WS. 1987. Phenoloxidase from shrimp (Penaeus setiferus): Purification and some properties. J Agric Food Chem 35: 918-921. https://doi.org/10.1021/jf00078a017
  7. Simpson BK, Marshall MR, Otwell WS. 2007. Phenoloxidases from pink and white shrimp: Kinetic and other properties. J Food Biochem 12: 205-218.
  8. Rolle RS, Guizani N, Chen JS, Marshall MR, Yang JS, Wei CI. 1991. Purification and characterization of phenoloxidase isoforms from taiwanese black tiger shrimp (Penaeus monodon). J Food Biochem 15: 17-32. https://doi.org/10.1111/j.1745-4514.1991.tb00141.x
  9. Gunnison AF, Jacobsen DW. 1987. Sulfite hypersensitivity. A critical review. CRC Crit Rev Toxicol 17: 185-214. https://doi.org/10.3109/10408448709071208
  10. Taylor SL, Higley NA, Bush RK. 1986. Sulfites in foods: uses, analytical methods, residues, fate, exposure assessment, metabolism, toxicity, and hypersensitivity. Adv Food Res 30: 1-76. https://doi.org/10.1016/S0065-2628(08)60347-X
  11. European Commission (EC). 2011. Commission regulation (EU) No 1129/2011 of 11 November 2011 amending Annex II to Regulation (EC) No 1333/2008 of the European Parliament and of the Council by establishing a Union list of food additives. Official Journal of the European Union L295: 1-177.
  12. Food and Drug Act. 1998. Regulations Amending the Food and Drug Regulations (1078). Canada Gazette Part II 132: 2695-2696.
  13. Frankos VH, Schmitt DF, Haws LC, McEvily AJ, Iyengar R, Miller SA, Munro IC, Clydesdale FM, Forbes AL, Sauer RM. 1991. Generally recognized as safe (GRAS) evaluation of 4-hexylresorcinol for use as a processing aid for prevention of melanosis in shrimp. Regul Toxicol Pharmacol 14: 202-212. https://doi.org/10.1016/0273-2300(91)90007-I
  14. USDA Foreign Agricultural Service. 2011. China-Peoples Republic of standards for uses of food additives - Part I. Gain Report No. CH11037, Washington, DC, USA. p 1-123.
  15. McEvily AJ, Iyengar R, Otwell S. 1991. Sulfite alternative prevents shrimp melanosis. Food Technol 45: 80-86.
  16. Guandalini E, Ioppolo A, Mantovani A, Stacchini P, Giovannini C. 1998. 4-Hexylresorcinol as inhibitor of shrimp melanosis: efficacy and residues studies; evaluation of possible toxic effect in a human intestinal in vitro model (Caco- 2); preliminary safety assessment. Food Addit Contam 15: 171-180. https://doi.org/10.1080/02652039809374627
  17. Montero P, Lopez-Caballero ME, Perez-Mateos M. 2001. The effect of inhibitors and high pressure treatment to prevent melanosis and microbial growth on chilled prawns (Penaeus japonicus). J Food Sci 66: 1201-1206.
  18. Jonker KM, Dekker CP. 2000. Determination of 4-hexylresorcinol in shrimp by liquid chromatography with fluorescence detection. J AOAC Int 83: 241-244.
  19. Encarnacion AB, Fagutao F, Shozen K, Hirono I, Ohshima T. 2011. Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus). Food Chem 127: 1594-1599. https://doi.org/10.1016/j.foodchem.2011.02.023
  20. Montero P, Martinez-Alvarez O, Zamorano JP, Alique R, Gomez-Guillen MC. 2006. Melanosis inhibition and 4-hexylresorcinol residual levels in deepwater pink shrimp (Parapenaeus longirostris) following various treatments. Eur Food Res Technol 223: 16-21. https://doi.org/10.1007/s00217-005-0080-4
  21. Ali M, Gleeson RA, Wei CI, Marshall MR. 1994. Activation mechanisms of pro-phenoloxidase on melanosis development in florida spiny lobster (Panulirus argus) cuticle. J Food Sci 59: 1024-1030. https://doi.org/10.1111/j.1365-2621.1994.tb08181.x
  22. Buda ES, Shafer TH. 2005. Expression of a serine proteinase homolog prophenoloxidase-activating factor from the blue crab, Callinectes sapidus. Comp Biochem Physiol B Biochem Mol Biol 140: 521-531. https://doi.org/10.1016/j.cbpc.2004.11.017
  23. Martinez-Alvarez O, Lopez-Caballero ME, Montero P, Gomez- Guillen MC. 2005. A 4-hexylresorcinol-based formulation to prevent melanosis and microbial growth in chilled tiger prawns (Marsupenaeus japonicus) from aquaculture. J Food Sci 70: M415-M422. https://doi.org/10.1111/j.1365-2621.2005.tb11469.x
  24. Zamorano JP, Martinez-Alvarez O, Montero P, Gomez- Guillen MC. 2009. Characterisation and tissue distribution of polyphenol oxidase of deepwater pink shrimp (Parapenaeus longirostris). Food Chem 112: 104-111. https://doi.org/10.1016/j.foodchem.2008.05.061