Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Immuno-Modulatory Activities of Polysaccharides Separated from Jubak in Macrophage Cells

주박(酒粕)에서 분리된 다당류의 대식세포 면역조절 활성

  • Park, Woo-Young (Department of Food Science and Technology, Kongju National University) ;
  • Sung, Nak-Yun (Department of Food Science and Technology, Kongju National University) ;
  • Byun, Eui-Hong (Department of Food Science and Technology, Kongju National University) ;
  • Oh, Kwang-Hoon (Department of Physical Education, College of Education, Kongju National University) ;
  • Byun, Myung-Woo (Department of Culinary Nutrition, Woosong University) ;
  • Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University)
  • 박우용 (공주대학교 식품공학과) ;
  • 성낙윤 (공주대학교 식품공학과) ;
  • 변의홍 (공주대학교 식품공학과) ;
  • 오광훈 (공주대학교 사범대학 체육교육과) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실)
  • Received : 2015.01.29
  • Accepted : 2015.06.02
  • Published : 2015.07.31
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Abstract

Activating macrophage cells play an important role in the host immune defense system. In this paper, immuno-modulatory activities of polysaccharides separated from Jubak (JPS) in macrophage cells were investigated. Immuno-modulatory activities were estimated based on cell proliferation, nitric oxide (NO) and cytokine production, degree of mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-${\kappa}B$ phosphorylation in RAW264.7 macrophage cells. JPS (62.5 to $250{\mu}g/mL$) did not induce a cytotoxic event. Additionally, NO and proinflammatory cytokines (tumor necrosis factor-${\alpha}$ and interleukin-6) production significantly increased in a dose-dependent manner. Similarly, phosphorylation of MAPKs and NF-${\kappa}B$ increased upon JPS treatment. Therefore, our results suggest that polysaccharides separated from Jubak can induce macrophage activation through MAPK and NF-${\kappa}B$ signaling and induction of Th1 polarization.

본 연구는 양조과정의 부산물인 주박으로부터 분리한 다당류(JPS)가 초기 면역반응에 중추적인 역할을 수행하는 대식세포에서 활성화를 유도하는지에 관한 여부를 알아보기 위해서 수행되었다. 주박에서 분리한 다당류를 마우스 유래 대식세포인 RAW264.7 cell에 처리하였을 때 대식세포의 활성화의 지표인 NO와 cytokine(IL-6, TNF-${\alpha}$)의 분비가 증가되었다. 또한 이러한 NO와 cytokine의 증가의 원인에 관한 면역기전에 관하여 알아본 결과 JPS의 처리는 MAPKs(ERK, JNK, p-38)의 인산화를 촉진시켜 NF-${\kappa}B$의 활성을 유도하여 면역세포의 활성인자들의 분비를 촉진시킨 것으로 관찰되었다.

Keywords

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