Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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A Study on Standardization of Shinbaro Pharmacopuncture Using Herbal Medicines Identification Test and HPLC-DAD

신바로 약침의 한약재 확인시험 및 HPLC-DAD를 통한 표준화 연구

  • Lee, Jin Ho (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Min Jeong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, Jae Woong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Me Riong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, In Hee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Eun Jee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation)
  • 이진호 (자생의료재단, 척추관절연구소) ;
  • 김민정 (자생의료재단, 척추관절연구소) ;
  • 이재웅 (자생의료재단, 척추관절연구소) ;
  • 김미령 (자생의료재단, 척추관절연구소) ;
  • 이인희 (자생의료재단, 척추관절연구소) ;
  • 김은지 (자생의료재단, 척추관절연구소)
  • Received : 2015.03.10
  • Accepted : 2015.06.01
  • Published : 2015.06.20
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Abstract

Objectives : The present study was an evaluation and standardization of herbal components in order to establish the efficacy and safety of Shinbaro pharmacopuncture. Methods : Among the raw materials of Shinbaro pharmacopuncture, the components Cibotii Rhizoma, Eucommiae Cortex, and Ledebouriellae Radix were assessed through ingredient verification experiments using thin-layer chromatography(TLC) and ultraviolet rays(UV) lamps. In addition, we standardized Acanthopanacis Cortex and Achyranthis Radix through validation using high performance liquid chromatograph-diode array detector(HPLC-DAD). Results : As result appeared a blue-white fluorescence under ultraviolet rays; changed to dark green after adding 1 % ferric chloride solution(due to Cibotii Rhizoma), and presented a yellow-green fluorescence when mixed with an ethyl ether under UV lamps by way of the ethyl ether layer, confirming Eucommiae Cortex. Ledebouriellae Radix was confirmed as dark brown spots at Rf values of 0.56 and 0.71 using TLC. Additionally, Acanthopanacis Cortex and Achyranthis Radix HPLC test results showed that linearity was $R^2{\geq}0.99$, and detection limit and quantitation limit were 0.23 to $1.29{\mu}g/mL$, and 0.71 to $3.90{\mu}g/mL$, respectively. Furthermore, precision and accuracy were confirmed to have relative standard deviation(RSD) values of 0.10 to 1.89 % and 96.19 to 103.72 %, respectively. Shinbaro pharmacopuncture did not have any overlapping or interference from other peaks in detection under the abovementioned analysis conditions. Conclusions : In conclusion, we confirmed that maintenance of Shinbaro pharmacopuncture validity was possible by means of quality control of Cibotii Rhizoma, Eucommiae Cortex, and Ledebouriellae Radix through ingredient identification and Acanthopanacis Cortex and Achyranthis Radix through high performance liquid chromatograph(HPLC) analysis. Further, we hope to contribute to the development strategy of herbal industry acupuncture.

Keywords

References

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