Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지
DOI QR코드

DOI QR Code

Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study

  • Lee, Hyo-Jung (Department of Periodontology, Section of Dentistry, Seoul National University Bundang Hospital) ;
  • Lee, Jaden (Department of Oral Health Sciences, Medical University of South Carolina College of Dental Medicine) ;
  • Lee, Jung-Tae (Department of Periodontology, Section of Dentistry, Seoul National University Bundang Hospital) ;
  • Hong, Ji-Soo (Department of Oral Pathology, Seoul National University School of Dentistry) ;
  • Lim, Bum-Soon (Department of Dental Biomaterials, Seoul National University School of Dentistry) ;
  • Park, Hee-Jung (Department of Public Health Sciences, Korea University) ;
  • Kim, Young-Kwang (Department of General Dentistry, Boston University School of Dental Medicine) ;
  • Kim, Tae-Il (Department of Periodontology, Seoul National University School of Dentistry)
  • Received : 2015.03.20
  • Accepted : 2015.05.25
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: With the significance of stable adhesion of alveolar bone and peri-implant soft tissue on the surface of titanium for successful dental implantation procedure, the purpose of this study was to apply microgrooves on the titanium surface and investigate their effects on peri-implant cells and tissues. Methods: Three types of commercially pure titanium discs were prepared; machined-surface discs (A), sandblasted, large-grit, acid-etched (SLA)-treated discs (B), SLA and microgroove-formed discs (C). After surface topography of the discs was examined by confocal laser scanning electron microscopy, water contact angle and surface energy were measured. Human gingival fibroblasts (hGFs) and murine osteoblastic cells (MC3T3-E1) were seeded onto the titanium discs for immunofluorescence assay of adhesion proteins. Commercially pure titanium implants with microgrooves on the coronal microthreads design were inserted into the edentulous mandible of beagle dogs. After 2 weeks and 6 weeks of implant insertion, the animal subjects were euthanized to confirm peri-implant tissue healing pattern in histologic specimens. Results: Group C presented the lowest water contact angle ($62.89{\pm}5.66{\theta}$), highest surface energy ($45{\pm}1.2mN/m$), and highest surface roughness ($Ra=22.351{\pm}2.766{\mu}m$). The expression of adhesion molecules of hGFs and MC3T30E1 cells was prominent in group C. Titanium implants with microgrooves on the coronal portion showed firm adhesion to peri-implant soft tissue. Conclusions: Microgrooves on the titanium surface promoted the adhesion of gingival fibroblasts and osteoblastic cells, as well as favorable peri-implant soft tissue sealing.

Keywords

References

  1. Branemark PI, Adell R, Albrektsson T, Lekholm U, Lundkvist S, Rockler B. Osseointegrated titanium fixtures in the treatment of edentulousness. Biomaterials 1983;4:25-8. https://doi.org/10.1016/0142-9612(83)90065-0
  2. Hermann JS, Buser D, Schenk RK, Higginbottom FL, Cochran DL. Biologic width around titanium implants. A physiologically formed and stable dimension over time. Clin Oral Implants Res 2000;11: 1-11. https://doi.org/10.1034/j.1600-0501.2000.011001001.x
  3. Geurs NC, Vassilopoulos PJ, Reddy MS. Soft tissue considerations in implant site development. Oral Maxillofac Surg Clin North Am 2010;22:387-405, vi-vii. https://doi.org/10.1016/j.coms.2010.04.001
  4. Abrahamsson I, Zitzmann NU, Berglundh T, Linder E, Wennerberg A, Lindhe J. The mucosal attachment to titanium implants with different surface characteristics: an experimental study in dogs. J Clin Periodontol 2002;29:448-55. https://doi.org/10.1034/j.1600-051X.2002.290510.x
  5. Huh JB, Rheu GB, Kim YS, Jeong CM, Lee JY, Shin SW. Influence of Implant transmucosal design on early peri-implant tissue responses in beagle dogs. Clin Oral Implants Res 2014;25:962-8. https://doi.org/10.1111/clr.12179
  6. Owens DK, Wendt RC. Estimation of surface free energy of polymers. J Appl Polym Sci 1969;13:1741-7. https://doi.org/10.1002/app.1969.070130815
  7. Bachle M, Kohal RJ. A systematic review of the influence of different titanium surfaces on proliferation, differentiation and protein synthesis of osteoblast-like MG63 cells. Clin Oral Implants Res 2004;15:683-92. https://doi.org/10.1111/j.1600-0501.2004.01054.x
  8. Cooper LF, Zhou Y, Takebe J, Guo J, Abron A, Holmen A, et al. Fluoride modification effects on osteoblast behavior and bone formation at TiO2 grit-blasted c.p. titanium endosseous implants. Biomaterials 2006;27:926-36. https://doi.org/10.1016/j.biomaterials.2005.07.009
  9. Kim MJ, Choi MU, Kim CW. Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell. Biomaterials 2006;27:5502-11. https://doi.org/10.1016/j.biomaterials.2006.06.023
  10. Marinucci L, Balloni S, Becchetti E, Belcastro S, Guerra M, Calvitti M, et al. Effect of titanium surface roughness on human osteoblast proliferation and gene expression in vitro. Int J Oral Maxillofac Implants 2006;21:719-25.
  11. Sader MS, Balduino A, Soares GD, Borojevic R. Effect of three distinct treatments of titanium surface on osteoblast attachment, proliferation, and differentiation. Clin Oral Implants Res 2005;16: 667-75. https://doi.org/10.1111/j.1600-0501.2005.01135.x
  12. Cochran DL, Hermann JS, Schenk RK, Higginbottom FL, Buser D. Biologic width around titanium implants. A histometric analysis of the implanto-gingival junction around unloaded and loaded nonsubmerged implants in the canine mandible. J Periodontol 1997;68:186-98. https://doi.org/10.1902/jop.1997.68.2.186
  13. Ruggeri A, Franchi M, Marini N, Trisi P, Piatelli A. Supracrestal circular collagen fiber network around osseointegrated nonsubmerged titanium implants. Clin Oral Implants Res 1992;3:169-75. https://doi.org/10.1034/j.1600-0501.1992.030403.x
  14. Broggini N, McManus LM, Hermann JS, Medina R, Schenk RK, Buser D, et al. Peri-implant inflammation defined by the implant-abutment interface. J Dent Res 2006;85:473-8. https://doi.org/10.1177/154405910608500515
  15. Chehroudi B, Gould TR, Brunette DM. The role of connective tissue in inhibiting epithelial downgrowth on titanium-coated percutaneous implants. J Biomed Mater Res 1992;26:493-515. https://doi.org/10.1002/jbm.820260407
  16. Linkevicius T, Apse P. Biologic width around implants. An evidence-based review. Stomatologija 2008;10:27-35.
  17. Gargiulo AW, Wentz FM, Orban B. Dimensions and relations of dentogingival junction in humans. J Periodontol 1961;32:261-7. https://doi.org/10.1902/jop.1961.32.3.261

Cited by

  1. Effects of alkaline treatment for fibroblastic adhesion on titanium vol.13, pp.6, 2016, https://doi.org/10.4103/1735-3327.197043
  2. Effect of Fibronectin-Coated Micro-Grooved Titanium Surface on Alignment, Adhesion, and Proliferation of Human Gingival Fibroblasts vol.23, pp.None, 2017, https://doi.org/10.12659/msm.903883
  3. Substrate roughness induces the development of defective E-cadherin junctions in human gingival keratinocytes vol.47, pp.2, 2015, https://doi.org/10.5051/jpis.2017.47.2.116
  4. Influence of surface treatment on PEDOT coatings: surface and electrochemical corrosion aspects of newly developed Ti alloy vol.8, pp.34, 2015, https://doi.org/10.1039/c8ra01718b
  5. The role of macrophage polarization on fibroblast behavior-an in vitro investigation on titanium surfaces vol.22, pp.2, 2015, https://doi.org/10.1007/s00784-017-2161-8
  6. The role of macrophage polarization on fibroblast behavior-an in vitro investigation on titanium surfaces vol.22, pp.2, 2015, https://doi.org/10.1007/s00784-017-2161-8
  7. Microgrooves and Microrugosities in Titanium Implant Surfaces: An In Vitro and In Vivo Evaluation vol.12, pp.8, 2015, https://doi.org/10.3390/ma12081287
  8. Enhancing osseointegration of titanium implants through large-grit sandblasting combined with micro-arc oxidation surface modification vol.30, pp.6, 2015, https://doi.org/10.1007/s10856-019-6276-0
  9. Titanium surface modifications and their soft-tissue interface on nonkeratinized soft tissues-A systematic review (Review) vol.14, pp.4, 2015, https://doi.org/10.1116/1.5113607
  10. A Study of Laser Micromachining of PM Processed Ti Compact for Dental Implants Applications vol.12, pp.14, 2015, https://doi.org/10.3390/ma12142246
  11. Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants vol.23, pp.8, 2019, https://doi.org/10.1007/s00784-018-2736-z
  12. Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants vol.23, pp.8, 2019, https://doi.org/10.1007/s00784-018-2736-z
  13. Construction of Complex Structures Containing Micro-Pits and Nano-Pits on the Surface of Titanium for Cytocompatibility Improvement vol.12, pp.17, 2015, https://doi.org/10.3390/ma12172820
  14. Viability and collagen secretion by fibroblasts on titanium surfaces with different acid-etching protocols vol.5, pp.1, 2015, https://doi.org/10.1186/s40729-019-0192-4
  15. Rotational UV-lithography using flexible chromium-coated polymer masks for the fabrication of microstructured dental implant surfaces: a proof of concept vol.30, pp.4, 2020, https://doi.org/10.1088/1361-6439/ab70c0
  16. A Novel Design of Temporomandibular Joint Prosthesis for Lateral Pterygoid Muscle Attachment: A Preliminary Study vol.8, pp.None, 2015, https://doi.org/10.3389/fbioe.2020.630983
  17. Histological and Histomorphometric Comparison of Innovative Dental Implants Laser Obtained: Animal Pilot Study vol.14, pp.8, 2021, https://doi.org/10.3390/ma14081830
  18. Corrosion Behavior and Biocompatibility of Diamond-like Carbon-Coated Zinc: An In Vitro Study vol.6, pp.14, 2015, https://doi.org/10.1021/acsomega.1c00531
  19. Enhancing the soft‐tissue integration of dental implant abutments—in vitro study to reveal an optimized microgroove surface design to maximize spreading and alignment of human gingival fib vol.109, pp.11, 2015, https://doi.org/10.1002/jbm.b.34836
  20. Fibroblasts Adhesion to Laser-Modified Titanium Surfaces-A Systematic Review vol.14, pp.23, 2015, https://doi.org/10.3390/ma14237305