Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Amelioration of Trimethyltin-induced Cognitive Impairment in ICR Mice by Perilla Oil

Trimethyltin 유도성 인지기능 저하 동물 모델에 대한 들기름의 개선효과

  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Bo Kyeong (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung, Tae Wan (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Chang Hyeon (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Sung Won (S&T FOODS Co. Ltd.) ;
  • Choi, Sung-Gil (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus).Institute of Agriculture and Life Science, Gyeongsang National University)
  • 강진용 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 박보경 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 승태완 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 박창현 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 진동은 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 강성원 (에스엔티푸드 주식회사) ;
  • 최성길 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부 (BK21 plus).농업생명과학연구원)
  • Received : 2015.01.19
  • Accepted : 2015.04.10
  • Published : 2015.06.30
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Abstract

This study aimed to investigate the anti-amnesic effect of perilla oil against trimethyltin (TMT)-induced learning and memory impairment in ICR mice. Perilla oil (2.5 mL/kg of body weight) and soybean oil (2.5 mL/kg of body weight) were administered orally to mice for 3 weeks, and at the end of the experimental period, cognitive behavior was examined by Y-maze and Morris water maze (MWM) tests. Behavioral tests showed that the mice treated with perilla oil had improved cognitive function compared to that in mice administered soybean oil. Analysis of brain tissue showed that perilla oil significantly lowered acetylcholinesterase activity and malondialdehyde (MDA) levels. Oxidized glutathione (GSH)-to-total GSH ratio also decreased from 10.4% to 5.3% in perilla oil-treated mice, but superoxide dismutase (SOD) activity increased from 11.7 to 14.2 U/mg protein. Therefore, these results suggest that the perilla oil could be a potential functional substance for improving cognitive function.

본 연구에서는 들기름의 TMT 유도성 인지 기능 상실에 대한 개선 효과와 함께 가정에서 식용유로서 사용량이 많은 대두유와 그 효과를 비교 연구하였다. 실험에서 들기름과 대두유를 섭취한 마우스를 TMT로 인지 기능 손상을 유발하여 Y-maze test와 Morris water maze test 한 결과, 공간 인지 기능 및 학습능력 개선에 대해 대두유는 효과가 미비하였으나 들기름은 대조군과 유의적인 차이가 없는 정도의 유의적 개선 효과를 보였다. 동물 실험 후 mouse로부터 적출된 뇌 조직을 대상으로 AChE 활성, MDA 함량, SOD 활성 측정 및 산화된 glutathione 측정한 결과, 대두유는 TMT 단독 처리군의 경우와 유사한 반면 들기름은 TMT에 의해 손상을 입은 mice의 뇌 조직에서 AChE의 활성과 MDA 생성 및 GSH의 산화를 억제시킬 뿐만 아니라 SOD 활성을 유의성 있게 증가시킨 것으로 나타났다. 이러한 결과는 들기름에 존재하는 풍부한 생리 활성 물질로서 ${\omega}$-3계 지방산, 페놀화합물 그리고 비타민 E 등에 의한 것으로 사료된다. 결국 들기름은 상대적으로 우수한 인지 기능 개선 효과를 나타냈고, 이는 건강기능 식품으로서 고부가가치 소재로 활용될 수 있는 산업적 활용 가능성을 나타내는 것으로 판단된다.

Keywords

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