DOI QR코드

DOI QR Code

Bleaching of Lipids Extracted from Single Cell Oil Produced by Mortierella sp.

모르티에렐라(Mortierella)속 유래 단세포유지로부터 추출한 지방질의 탈색

  • Received : 2015.03.18
  • Accepted : 2015.04.20
  • Published : 2015.06.30

Abstract

The deacidified oil obtained from the oleaginous fungus, Mortierella sp. (M-12) was bleached, after degumming, using activated clay under a 50-100 mmHg vacuum. The bleaching conditions were partially optimized as follows: activated clay, 1%, bleaching temperature $90^{\circ}C$, and treatment time 20 min. After bleaching, the color of bleached oil as determined by the Lovibond Tintometer, satisfied the specification for edible fats and oils. The bleaching process also decreased the contents of free fatty acids and phosphorus in the deacidified oil. The acid value of the bleached oil also satisfied the specification for edible fats and oils. It was early shown that the normal bleaching process can be used for the bleaching of heavily-colored microbial lipids for human consumption.

아라키돈산을 고농도로 함유한 유지를 생산하는 Mortierella sp. M-12로부터 얻은 균체 지방질을 식용에 적합하도록 정제하기 위하여 탈색 공정을 최적화하였다. 동결건조한 균체로부터 Folch's solvent를 사용하여 균체 지방질 조유를 얻은 후 탈검과 탈산을 거쳐 얻은 탈산유를 50-100 mmHg 진공 하에서 탈색을 진행하였다. 활성백토를 1% 수준으로 첨가하여 $90^{\circ}C$에서 20분간 수행하였을 때 식용유지 규격에 적합한 색상을 갖는 탈색유를 얻을 수 있었다. 탈색 공정 중에 부수적으로 일어난 탈검과 탈산 작용으로 인하여 탈색유의 인 함량은 31.7% 감소하였으며, 산값은 0.5로 감소하여 식용유지의 규격에 적합한 수준이 되었다. 모르티에렐라 단세포유지는 일반적인 유지의 탈색공정을 통하여 식용급 동식물 유지와 같은 수준의 탈색유를 얻을 수 있었다.

Keywords

References

  1. Brash AR. Arachidonic acid as a bioactive molecule. J. Clin. Invest. 107: 1339-1345 (2001) https://doi.org/10.1172/JCI13210
  2. Shinmen Y, Shimizu S, Akimoto K, Kawashima H, Yamada H. Production of arachidonic acid by Mortierella fungi. App. Microbiol. Biot. 31: 11-16 (1989)
  3. Kyle D. Arachidonic acid and methods for the production and use thereof. U.S. Patent 5,658,767 (1997)
  4. Jareonkitmongkol S, Sakuradani E, Shimizu S. A novel 5-desaturase- defective mutant of Mortierella alpina 1S-4 and its dihomo- -linolenic acid productivity. Appl. Environ. Microb. 59: 4300-4304 (1993)
  5. Jung MY, Yoon SH, Min DB. Effects of processing steps on the contents of minor compounds and oxidation of soybean oil. J. Am. Oil Chem. Soc. 66: 118-120 (1989) https://doi.org/10.1007/BF02661798
  6. Dueppen DG, Zeller SG, Diltz SI, Driver RH. Extraction and winterization of lipids from oilseed and microbial sources. U.S. Patent 7,695,626 (2010)
  7. Gil B, Kim MJ, Kim JH, Yoon SH. Comparative Study of Soybean Oil refining using rice hull silicate and commercial adsorbents. Food Sci. Biotechnol. 23: 1025-1028 (2014) https://doi.org/10.1007/s10068-014-0139-8
  8. Ortega-Garcia J, Medina-Juarez LA, Gamez-Meza N, Noriega-Rodriguez JA. Optimisation of bleaching conditions for soybean oil using response surface methodology. Food Sci. Technol. Int. 11: 443-449 (2005) https://doi.org/10.1177/1082013205060677
  9. Kim SK, Chung GH, Han JJ, Cho SW, Yoon SH. Effect of extraction methods on the extraction yield of total lipid and arachidonic acid from single cell oil, Mortierella sp. Korean J. Food Sci. Technol. 47: 281-285 (2015) https://doi.org/10.9721/KJFST.2015.47.3.281
  10. Liu KS. Preparation of fatty acid methyl esters for gas-chromatographic analysis of lipids in biological materials. J. Am. Oil Chem. Soc. 71: 1179-1187 (1994) https://doi.org/10.1007/BF02540534
  11. AOCS. Official Methods and Recommended Practices of the AOCS. 6th ed. Method Ce2-66, Cd 3d-63, Cd 8-53, Ca 12-55, Cc 13e. American Oil Chemists' Society, Champaign, IL, USA (2009)
  12. Lee SY, Jung MY, Yoon SH. Optimization of the refining process of camellia seed oil for edible purposes. Food Sci. Biotechnol. 23: 65-73 (2014) https://doi.org/10.1007/s10068-014-0009-4
  13. Ott L. Introduction to the analysis of variance. pp. 325-360. In: An Introduction to Statistical Methods and Data Analysis. Bogartz RS (ed). PWS Publishers, Boston, MA, USA (1984)
  14. Yuan C, Wang J, Yao J, Yu Z, Shang Y, Gong G. Production of arachidonic acid by Mortierella alpina $I_{49}-N_{18}$. Food Technol. Biotech. 40: 311-315 (2002)
  15. Skevin D, Domijan T, Kraljic K, Kljusuric JG, Nederal S, Obranovic M. Optimization of bleaching parameters for soybean oil. Food Technol. Biotech. 50: 199-207 (2012)
  16. List GR, King JW, Johnson JH, Warner K, Mounts TL. Supercritical $CO_2$ degumming and physical refining of soybean oil. J. Am. Oil Chem. Soc. 70: 473-476 (1993) https://doi.org/10.1007/BF02542578
  17. Wan PJ, Hurley TW, Guy JD, Berner DL. Comparison of visual and automated colorimeters-An international collaborative study. J. Am. Oil Chem. Soc. 74: 731-738 (1997) https://doi.org/10.1007/s11746-997-0210-1
  18. Wiedermann LH. Degumming, refining and bleaching soybean oil. J. Am. Oil Chem. Soc. 58: 159-166 (1981) https://doi.org/10.1007/BF02582328
  19. Taylor DR, Jenkins DB, Ungermann CB. Bleaching with alternative layered minerals: A comparison with acid-activated montmorillonite for bleaching soybean oil. J. Am. Oil Chem. Soc. 66: 334-341 (1989) https://doi.org/10.1007/BF02653285
  20. Maza A, Ormsbee RA, Strecker LR. Effects of deodorization and steam-refining parameters on finished oil quality. J. Am. Oil Chem. Soc. 69: 1003-1008 (1992) https://doi.org/10.1007/BF02541066

Cited by

  1. Single Cell Oil-Recent Trends in Microbial Production and Utilization vol.47, pp.6, 2015, https://doi.org/10.9721/KJFST.2015.47.6.687