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Micromorphology and development of the epicuticular structure on the epidermal cell of ginseng leaves

  • Lee, Kyounghwan (Department of Cell and Developmental Biology, University of Massachusetts Medical School) ;
  • Nah, Seung-Yeol (College of Veterinary Medicine, Konkuk University) ;
  • Kim, Eun-Soo (Department of Biological Sciences, Konkuk University)
  • Received : 2014.04.19
  • Accepted : 2014.10.15
  • Published : 2015.04.15

Abstract

Background: A leaf cuticle has different structures and functions as a barrier to water loss and as protection from various environmental stressors. Methods: Leaves of Panax ginseng were examined by scanning electron microscopy and transmission electron microscopy to investigate the characteristics and development of the epicuticular structure. Results: Along the epidermal wall surface, the uniformly protuberant fine structure was on the adaxial surface of the cuticle. This epicuticular structure was highly wrinkled and radially extended to the marginal region of epidermal cells. The cuticle at the protuberant positions maintained the same thickness. The density of the wall matrix under the structures was also similar to that of the other wall region. By contrast, none of this structure was distributed on the abaxial surface, except in the region of the stoma. During the early developmental phase of the epicuticular structure, small vesicles appeared on wallecuticle interface in the peripheral wall of epidermal cells. Some electron-opaque vesicles adjacent to the cuticle were fused and formed the cuticle layer, whereas electron-translucent vesicles contacted each other and progressively increased in size within the epidermal wall. Conclusion: The outwardly projected cuticle and epidermal cell wall (i.e., an epicuticular wrinkle) acts as a major barrier to block out sunlight in ginseng leaves. The small vesicles in the peripheral region of epidermal cells may suppress the cuticle and parts of epidermal wall, push it upward, and consequently contribute to the formation of the epicuticular structure.

Keywords

References

  1. Borve J, Sekse L, Stensvand A. Cuticular fractures promote postharvest fruit rot in sweet cherries. Plant Disease 2000;84:1180-4. https://doi.org/10.1094/PDIS.2000.84.11.1180
  2. Kosma DK, Bourdenx B, Bernard A, Parsons EP, Lu S, Joubes J, Jenks MA. The impact of water deficiency on leaf cuticle lipids of Arabidopsis. Plant Physiology 2009;151:1918-29. https://doi.org/10.1104/pp.109.141911
  3. Khajuria C, Wang H, Liu X, Wheeler S, Reese J, El Bouhssini M, Whitworth R, Chen M-S. Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against hessian fly. BMC Genomics 2013;14:423-38. https://doi.org/10.1186/1471-2164-14-423
  4. Koch K, Ensikat HJ. The hydrophobic coatings of plant surfaces: epicuticular wax ctystals and their morphologies, crystallinity and molecular self-assembly. Micron 2008;39:759-72. https://doi.org/10.1016/j.micron.2007.11.010
  5. Reina-Pinto JJ, Yephremov A. Surface lipids and plant defenses. Plant Physiol Biochem 2009;47:540-9. https://doi.org/10.1016/j.plaphy.2009.01.004
  6. Werker E. Trichome diversity and development. Adv Bot Res 2000;31:1-35. https://doi.org/10.1016/S0065-2296(00)31005-9
  7. Chen G, Komatsuda T, Ma JF, Li C, Yamaji N, Nevo E. A functional cutin matrix is required for plant protection against water loss. Plant Signal Behav 2011;6: 1297-9. https://doi.org/10.4161/psb.6.9.17507
  8. Budke JM, Goffinet B, Jones CS. The cuticle on the gametophyte calyptra matures before the sporophyte cuticle in the moss Funaria hygrometrica (Funariaceae). Amer J Bot 2012;99:14-22. https://doi.org/10.3732/ajb.1100311
  9. Buschhaus C, Jetter R. Composition differences between epicuticular and intracuticular wax substructures: how do plants seal their epidermal surfaces? J Exp Bot 2011;62:841-53. https://doi.org/10.1093/jxb/erq366
  10. Kim KW, Ahn JJ, Lee JH. Micromorphology of epicuticular wax structures of the garden strawberry leaves by electron microscopy: syntopism and polymorphism. Micron 2009;40:327-34. https://doi.org/10.1016/j.micron.2008.11.002
  11. Nawrath C. Unraveling the complex network of cuticular structure and function. Curr Opin Plant Biol 2006;9:281-7. https://doi.org/10.1016/j.pbi.2006.03.001
  12. Parsons EP, Popopvsky S, Lohrey GT, Lu S, Alkalai-Tuvia S, Perzelan Y, Paran I, Fallik E, Jenks MA. Fruit cuticle lipid composition and fruit post-harvest water loss in an advanced backcross generation of pepper (Capsicum sp.). Physiol Planta 2012;146:15-25. https://doi.org/10.1111/j.1399-3054.2012.01592.x
  13. Riederer M, Schonherr J. Development of plant cuticles: fine structure and cutin composition of Clivia miniata Reg. leaves. Planta 1988;174:127-38. https://doi.org/10.1007/BF00394885
  14. Yeats TH, Rose JK. The formation and function of plant cuticles. Plant Physiol 2013;163:5-20. https://doi.org/10.1104/pp.113.222737
  15. Onoda Y, Richards L, Westoby M. The importance of leaf cuticle for carbon economy and mechanical strength. New Phytol 2012;196:441-7. https://doi.org/10.1111/j.1469-8137.2012.04263.x
  16. Holloway PJ. Structure and histochemistry of plant cuticular membranes: an overview. In: Cutler DF, Alvin KL, Price CE, editors. The plant cuticle. London, UK: Academic Press; 1982.
  17. Heredia-Guerrero JA, de Lara R, Dominguez E, Heredia A, Benavente J, Benitez JJ. Chemical-physical characterization of isolated plant cuticles subjected to low-dose $\gamma$-irradiation. Chem Phys Lipid 2012;165:803-8. https://doi.org/10.1016/j.chemphyslip.2012.10.003
  18. Schreiber L. Transport barriers made of cutin, suberin and associated waxes. Trends Plant Sci 2010;15:546-53. https://doi.org/10.1016/j.tplants.2010.06.004
  19. Boardman NK. Comparative photosynthesis of sun and shade plants. Ann Rev Plant Physiol 1977;28:355-77. https://doi.org/10.1146/annurev.pp.28.060177.002035
  20. Lee JS, Lee KH, Lee SS, Kim ES, Ahn IO, In JG. Morphological characteristics of ginseng leaves in high-temperature injury resistant and susceptible lines of Panax ginseng Meyer. Ginseng Res 2011;35:449-56. https://doi.org/10.5142/jgr.2011.35.4.449
  21. Reynolds ES. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 1963;17:208-12. https://doi.org/10.1083/jcb.17.1.208
  22. Jeffree CE. Structure and ontogeny of plant cuticles. In: Kerstiens G, editor. Plant cuticle: an integrated functional approach. Oxford, England: BIOS; 1996. p. 33-82.
  23. Kim ES, Mahlberg PG. Glandular cuticle formation of Cannabis (Cannabaceae). Amer J Bot 1995;82:1207-14. https://doi.org/10.2307/2446242
  24. Yeats TH, Buda GJ, Wang Z, Chehanovsky N, Moyle LC, Jetter R, Schaffer AA, Rose JK. The fruit cuticles of wild tomato species exhibit architectural and chemical diversity, providing a new model for studying the evolution of cuticle function. Plant J 2012;69:655-66. https://doi.org/10.1111/j.1365-313X.2011.04820.x
  25. Dickison WC, Weitzman AL. Comparative anatomy of the young stem, node, and leaf of the Bonnetiaceae, including observations on a foliar endodermis. Amer J Bot 1996;83:405-18. https://doi.org/10.2307/2446210
  26. Bret-Harte MS, Talbott L. Changes in composition of the outer epidermal cell wall of pea stems during auxin-induced growth. Planta 1993;190:369-78.
  27. Mahlberg PG, Kim ES. Cuticle development on glandular trichomes of Cannabis sativa L. (Cannabaceae). Amer J Bot 1991;78:1113-22. https://doi.org/10.2307/2444899
  28. Baker EA, Hunt GM. Erosion of waxes from leaf surfaces by simulated rain. New Phytol 1986;102:161-73. https://doi.org/10.1111/j.1469-8137.1986.tb00807.x
  29. Bernard A, Joubes J. Arabidopsis cuticular waxes: advances in synthesis, export and regulation. Prog Lipid Res 2013;52:110-29. https://doi.org/10.1016/j.plipres.2012.10.002
  30. Riedel M, Eichner A, Meimberg H, Jetter R. Chemical composition of epicuticular wax crystals on the slippery zone in pitchers of five Nepenthes species and hybrids. Planta 2007;225:1517-34. https://doi.org/10.1007/s00425-006-0437-3
  31. Kim ES, Mahlberg PG. Secretory vesicle formation in the secretory cavity of glandular trichomes of Cannabis sativa L. (Cannabaceae). Mol Cell 2003;15:387-95.

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