Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats

  • Kim, Kyung-Ah (Department of Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Yoo, Hye Hyun (Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University) ;
  • Gu, Wan (Department of Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Yu, Dae-Hyung (Department of Pharmacy, College of Pharmacy, Kyung Hee University) ;
  • Jin, Ming Ji (Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University) ;
  • Choi, Hae-Lim (Roquette Korea Ltd.) ;
  • Yuan, Kathy (Roquette Management (Shanghai) Co., Ltd.) ;
  • Guerin-Deremaux, Laetitia (Biology and Nutrition Department, Roquette Freres) ;
  • Kim, Dong-Hyun (Department of Pharmacy, College of Pharmacy, Kyung Hee University)
  • Received : 2014.07.03
  • Accepted : 2014.11.07
  • Published : 2015.04.15
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Abstract

Background: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics of ginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. Methods: Male Sprague-Dawley rats were given control or NUTRIOSE-containing diets (control diet + NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood samples were collected to investigate the pharmacokinetics of CK using liquid chromatography-tandem mass spectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimens or bacteria cultures. Results: When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSE containing diets, the maximum plasma concentration ($C_{max}$) and area under the plasma concentration-time curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSE intake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activities of intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. Conclusion: These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolic conversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract and may potentiate the pharmacological effects of ginseng.

Keywords

References

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