Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Oligonol, a Low Molecular Weight Polyphenol Derived from Lychee on Oxidative Stress-Related Hepatic Damage in Streptozotocin-Induced Diabetic Rats

리치 저분자 폴리페놀인 Oligonol의 Streptozotocin 투여 당뇨 쥐에서 산화적 스트레스와 관련된 간 손상에 미치는 영향

  • Noh, Jeong Sook (Department of Food Science & Nutrition, Tongmyong University)
  • 노정숙 (동명대학교 식품영양학과)
  • Received : 2014.11.28
  • Accepted : 2015.01.28
  • Published : 2015.04.30
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Abstract

This study was conducted to examine whether or not oligonol, a low molecular weight polyphenol derived from lychee fruit, has an ameliorative effect on diabetes-induced oxidative stress-related hepatic damage in streptozotocin (STZ)-induced diabetic rats. Oligonol (10 or 20 mg/kg body weight; O10 or O20, respectively) was orally administered every day for 10 days to STZ-induced diabetic rats, and its effects were compared to vehicle-treated diabetic (Veh) and non-diabetic rats. Administration of 20 mg/kg of oligonol significantly decreased liver weight compared with the Veh group (P<0.05). Elevated levels of hepatic glucose, reactive oxygen species, peroxynitrite, and lipid peroxidation were detected in diabetic vehicle rats, whereas oligonol treatment significantly attenuated these levels (P<0.05). In diabetic vehicle rats, hepatic antioxidant enzyme protein levels decreased, whereas oligonol treatment showed significant elevated results. For inflammation-related protein expression, oligonol-treated groups showed insignificant reduction. Oligonol improved expression of proapoptotic protein caspase-3 in the liver of diabetic rats (P<0.05). In conclusion, these results provide important evidence that oligonol exhibits an inhibitory effect on oxidative stress and apoptosis-related protein expression as well as a hepato-protective effect against the development of diabetic complications in STZ-induced type 1 diabetic rats.

본 연구에서는 STZ 유발 당뇨 쥐에서 고혈당 및 산화적 스트레스 관련 간 손상 기전에 oligonol의 영향에 대해 살펴보고자 하였다. 10일 동안 oligonol을 투여한 결과 20 mg/kg oligonol 투여군에서 당뇨대조군에 비해 간 무게 및 간조직의 glucose, ROS, peroxynitrite, 지질과산화물 농도가 유의적으로 감소하였다. 간조직의 항산화 효소 단백질 발현을 측정한 결과 정상군에 비해 당뇨대조군에서 발현 정도가 낮아졌지만 oligonol 투여군에서는 유의적으로 증가하였다. 또한 caspase-3 효소의 단백질 발현은 당뇨대조군에서 증가하였으나 oligonol 투여군에서 그 발현이 억제되었다. 따라서 oligonol의 투여는 STZ-유발 당뇨 모델에서 ROS 및 지질과산화물 생성 억제와 항산화효소 작용의 증가 및 세포 사멸 작용이 있는 caspase-3 발현 억제를 통해 고혈당 및 산화적 스트레스에 의해 유발되는 간 손상에 대한 보호 효과가 있는 것으로 사료된다.

Keywords

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