Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Extract Mixture (Yg-1) of Anti-Inflammatory Herbs on LPS-Induced Acute Inflammation in Macrophages and Rats

급성염증성 발열 모델에서의 항염증성 약재 혼합 추출물(YG-1)의 효과

  • Song, In-Bong (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University) ;
  • Na, Ji-Young (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University) ;
  • Song, Kibbeum (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Sokho (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Ji-Hyun (Department of Immunology, Institute for Medical Science, Chonbuk National University) ;
  • Kwon, Young-Bae (Department of Pharmacology, Institute for Medical Science, Chonbuk National University) ;
  • Kim, Dae-Ki (Department of Immunology, Institute for Medical Science, Chonbuk National University) ;
  • Kim, Dae-Sung (Central Research and Development, Hanpoong Pharm & Foods) ;
  • Jo, Hyoung-Kwon (Central Research and Development, Hanpoong Pharm & Foods) ;
  • Kwon, Jungkee (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University)
  • 송인봉 (전북대학교 수의과대학 실험동물의학교실) ;
  • 나지영 (전북대학교 수의과대학 실험동물의학교실) ;
  • 송기쁨 (전북대학교 수의과대학 실험동물의학교실) ;
  • 김석호 (전북대학교 수의과대학 실험동물의학교실) ;
  • 이지현 (전북대학교 의학전문대학원 면역학교실) ;
  • 권영배 (전북대학교 의학전문대학원 약리학교실) ;
  • 김대기 (전북대학교 의학전문대학원 면역학교실) ;
  • 김대성 (한풍제약) ;
  • 조형권 (한풍제약) ;
  • 권중기 (전북대학교 수의과대학 실험동물의학교실)
  • Received : 2014.11.27
  • Accepted : 2014.12.24
  • Published : 2015.04.30
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Abstract

Traditional herbs, such as Lonicera japonica, Arctii Fructus, and Scutellariae Radix have been used as traditional drug due to their anti-inflammatory and anti-oxidant activities. The aim of this study was to investigate the anti-inflammatory effects of extract mixture (YG-1) in a model of lipopolysaccharide (LPS)-induced acute inflammation in both macrophage (RAW 264.7) cells and Sprague-Dawley rats. YG-1 did not show specific cellular toxicity in RAW 264.7 cells until a concentration of $100{\mu}g/mL$. YG-1 reduced various markers related to inflammation such as IL-$1{\beta}$, COX-2, and iNOS caused by LPS in RAW 264.7 cells. Consistent with these results, YG-1 exerted significant anti-inflammatory effects in an acute inflammation rat model. Acute fever and high concentration of IL-$1{\beta}$ in serum induced by LPS were significantly reduced by YG-1. These results were similar to flubiprofen, a commercial anti-inflammatory and anti-febrile drug. Therefore, these results indicate that YG-1 has beneficial effects on LPS-induced acute inflammation and suggest that YG-1 can serve as an effective anti-inflammatory and anti-febrile drug.

YG-1의 항염증 및 해열 치료 약물로서의 가능성을 검증하기 위하여 대식세포와 동물모델에서 염증성 사이토카인 활성 및 해열 관련 인자들을 연구하여 다음과 같은 결론을 얻었다. YG-1은 염증성 사이토카인 인자인 IL-$1{\beta}$의 발현을 효과적으로 억제하였으며, iNOS 및 COX-2의 발현을 억제시킴으로써 염증인자인 NO 및 $PGE_2$ 생성을 유의적으로 감소시키는 것으로 나타났다. 또한 YG-1은 LPS 유도성 발열 모델에서 염증을 완화시키고 해열의 효과를 보여 주었다. 이상의 실험 결과로부터 본 실험에 사용된 YG-1은 염증에 의해 유발되는 인후염과 같은 질환을 예방 및 치료할 수 있을 것으로 기대된다.

Keywords

References

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