Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Pomegranate Extract Improves Menopausal Syndrome in Ovariectomized Rats

난소 적출 동물모델에서 고흥산 석류 농축액의 갱년기 증상 개선 효과

  • Wee, Ji-Hyang (Department of Research and Development, Jeonnam Food Research Institute) ;
  • Jung, Hyun Jung (Department of Research and Development, Jeonnam Food Research Institute) ;
  • Jung, Kyung Ok (Department of Research and Development, Jeonnam Food Research Institute) ;
  • Sung, Hea Mi (Department of Research and Development, Jeonnam Food Research Institute) ;
  • Shin, Yu-Rim (BioFoodstory Co., Ltd.) ;
  • Park, Ju-Hyun (Chonbuk National University Medical School) ;
  • Seo, Hyeon-Young (BioFoodstory Co., Ltd.) ;
  • Lim, Jung-Min (Chonbuk National University Medical School) ;
  • Chae, Han-Jeong (Chonbuk National University Medical School) ;
  • Lee, Ki Yung (Department of Agriculture Economics, Suncheon National University)
  • 위지향 ((재)전남생물산업진흥원 식품산업연구원) ;
  • 정현정 ((재)전남생물산업진흥원 식품산업연구원) ;
  • 정경옥 ((재)전남생물산업진흥원 식품산업연구원) ;
  • 성혜미 ((재)전남생물산업진흥원 식품산업연구원) ;
  • 신유림 ((주)바이오푸드스토리) ;
  • 박주현 (전북대학교 의학전문대학원) ;
  • 서현영 ((주)바이오푸드스토리) ;
  • 임정민 (전북대학교 의학전문대학원) ;
  • 채한정 (전북대학교 의학전문대학원) ;
  • 이기웅 (순천대학교 농업경제학과)
  • Received : 2014.12.04
  • Accepted : 2015.01.12
  • Published : 2015.04.30
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Abstract

In this study, the effect of Goheung pomegranate extract on postmenopausal syndrome was evaluated both in vitro and in vivo in ovariectomized Sprague-Dawly (SD) rats. Sixty female SD rats were divided into six groups: sham, sham operation and distilled water; OVX, ovariectomized and distilled water; PE1, ovariectomized and pomegranate concentrate (0.75 mL/twice/d); PE2, ovariectomized and pomegranate concentrate (1.5 mL/twice/d); PE3, ovariectomized and pomegranate concentrate (2.2 mL/twice/d); and CE, ovariectomized and commercial pomegranate concentrate (2.2 mL/twice/d). Percent bone volume (bone volume/tissue volume) and trabecular thickness (Tb.Th) improved in a dose-independent manner in PE1, 2, and 3. Especially, bone mineral density was significantly improved in PE3 (P<0.05) compared to OVX. Pomegranate extract reduced body weight and visceral fat mass. High density lipoprotein cholesterol (HDL-C) level slightly increased in a dose-independent manner in the experimental group. In addition, HDL-C/total cholesterol level of PE3 significantly increased (P<0.05) compared with OVX. These results show that pomegranate concentrate improved blood lipid levels and bone metabolism in ovariectomized rats. Therefore, Goheung pomegranate concentrates are expected to improve cardiovascular and bone-related diseases in menopausal women.

본 연구는 난소 적출 동물 모델을 이용하여 고흥산 석류 농축액 시험군과 함께 이란산 석류 농축액을 주성분으로 하는 시판 제품의 혈청 생화학적 지표, 골밀도 등 몇 가지 지표를 분석하여 갱년기 증상 개선 효과를 비교하였다. 고흥산 석류 농축액과 시판 제품은 난소 절제 동물 모델의 골밀도(bone mineral density, BMD) 및 percent bone volume(bone volume/tissue volume), bone surface density(bone surface/tissue volume)를 증가시켰으며, 특히 고용량의 고흥산 석류 농축액(PE3)은 통계적으로 유의하게 골밀도 및 골볼륨을 증가시켰다. 시판 제품을 투여한 군도 골밀도를 증가시키는 양상을 보였으나 통계적 유의성은 없어 고흥산 석류 농축액이 이란산 석류 제품보다 골밀도 등 뼈 건강에 관련한 갱년기 증상 개선에 더 효과적이라고 사료된다. 고흥산 석류 농축액은 난소 절제에 의한 체중 증가 및 복부/내장지방 축적을 개선하였으며, 농도 의존적으로 HDL-C(high density lipoprotein cholesterol)는 증가시키고 low density lipoprotein cholesterol은 감소시켰다. 또한 고농도 석류 농축액(PE3)의 HDL-C/total cholesterol이 통계적으로 유의하게 증가하여 고흥산 석류 농축액이 갱년기 여성의 비만 및 혈중지질을 개선할 수 있을 것으로 기대된다. 불안장애 실험에서는 고흥산 석류 농축액 투여로 인해 불안장애가 개선되는 경향을 보였으나 통계적 유의성이 없었고, 이란산 시판 제품 투여 시에는 대조군에 비해 유의하게 개선시키는 것으로 나타났다. 우울증 또한 시판 제품 투여군에서는 대조군에 비해 유의하게 개선 효과를 나타내었으나 고흥산 석류 농축액 투여군에서는 우울증 개선 효과가 확인되지 않았다. 이상과 같은 결과를 통해 고흥산 석류 농축액은 난소를 적출한 rat의 골 소실을 현저히 개선하고 복부/내장지방 및 혈중지질을 농도 의존적으로 개선하여 골다공증, 비만 및 심혈관 질환과 같은 갱년기 증상에 효과가 있음을 확인하였다. 그러나 고흥산 석류 농축액 투여군에서 대표적인 갱년기 증상인 우울증과 불안장애 개선에 유의성 있는 효과가 나타나지 않는 것은 동물실험 모델에서 사람에게 나타나는 다양한 갱년기 증상을 모두 평가하기에 한계가 있는 바, 향후 추가적인 연구를 통해 고흥산 석류 농축액이 갱년기 여성에게 나타나는 여러 가지 증상 중 특정 증상에 대해 선택적으로 효과를 나타내는지에 대해 임상시험을 통해 검증할 필요가 있겠다.

Keywords

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