Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Chemical Properties and Immuno-Stimulating Activities of Crude Polysaccharides from Enzyme Digests of Tea Leaves

녹차 효소 처리 다당의 화학적 특성 및 면역증진 활성

  • Park, Hye-Ryung (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Suh, Hyung Joo (Department of Integrated Biomedical and Life Science, College of Health Science, Korea University) ;
  • Yu, Kwang-Won (Department of Food and Nutrition, Korea University of Transportation) ;
  • Kim, Tae Young (Bionic Trading Corporation) ;
  • Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University)
  • 박혜령 (경기대학교 식품생물공학과) ;
  • 서형주 (고려대학교 보건과학대학 의생명융합과학과) ;
  • 유광원 (한국교통대학교 식품영양학과) ;
  • 김태영 ((주)비티씨) ;
  • 신광순 (경기대학교 식품생물공학과)
  • Received : 2014.12.29
  • Accepted : 2015.04.09
  • Published : 2015.05.31
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Abstract

In order to develop new immuno-stimulating ingredients from mature leaves of green tea, crude polysaccharides were isolated from pectinase digests of tea leaves (green tea enzyme digestion, GTE-0), after which their immuno-stimulating activities and chemical properties were examined. GTE-0 mainly contained neutral sugars (54.9%) such as glucose (14.2%), arabinose (12.2%), rhamnose (11.1%), and galacturonic acid (45.1%), which are characteristic of pectic polysaccharides. The anti-complementary activity of GTE-0 was similar to that of polysaccharide K (used as positive control). Number of morphologically activated macrophages was significantly increased in the GTE-0-treated group. GTE-0 significantly augmented $H_2O_2$ and reactive oxygen species production by murine peritoneal macrophage cells in a dose-dependent manner, whereas production of nitric oxide showed the highest activity at a dose of $100{\mu}g/mL$ among all tested concentrations. Murine peritoneal macrophages stimulated with GTE-0 showed enhanced production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factors-${\alpha}$ in a dose-dependent manner. Further, GTE-0 induced higher phagocytic activity in a dose-dependent manner. In ex vivo assay for cytolytic activity of murine peritoneal macrophages, GTE-0-treated group showed significantly higher activity compared to the untreated group at an effector-to-target cell ratio of 20. The above results lead us to conclude that polysaccharides from leaves of green tea have a potent immuno-stimulating effect on murine peritoneal macrophage cells.

녹차 성숙잎으로부터 새로운 면역 활성 다당 소재를 개발할 목적으로 녹차잎을 pectinase로 처리하여 조다당 GTE-0을 분리하고 이들의 면역증진 활성과 화학적 특성에 대해 조사하였다. GTE-0은 중성당 54.9%, 산성당 45.1%로 이루어져 있었으며, 구성당 분석 결과 주로 glucose(14.2%), arabinose(12.2%), rhamnose(11.1%) 및 galactose(7.3%)로 구성되어 있었다. 한편 GTE-0은 비특이적 면역계에 있어 중요한 역할을 담당하고 있는 보체계에 대하여 양성대조군 PSK에 준하는 우수한 활성이 농도 의존적으로 나타났다. 또한 GTE-0을 처리하고 검경 시 형태적으로 구분이 가능한 활성화된 대식세포의 숫자가 증가되는 경향을 보였다. 대식세포의 NO, ROS 및 $H_2O_2$ 생산에 미치는 GTE-0의 효과를 검토한 결과 ROS와 $H_2O_2$는 모두 농도 의존적으로 생산량을 증가시키는 우수한 활성을 나타낸 반면, NO의 생산능은 1,000 mg/mL의 고농도에서보다 오히려 100 mg/mL의 저농도에서 더 우수한 활성을 나타내었다. 또한 GTE-0으로 자극한 대식세포는 무처리 대조군에 비해 IL-6, IL-12 및 TNF-${\alpha}$와 같은 다양한 cytokine들의 생산이 농도 의존적으로 증가되는 경향을 보였다. 대식세포의 식작용 활성을 측정한 결과 무처리 대조군에 비해 GTE-0 100 mg/mL 농도이상 처리하였을 때 우수한 활성을 나타내었다. 또한 활성화된 대식세포의 YAC-1 종양세포주에 대한 치사 활성을 ex vivo로 측정한 결과 100 mg/mL의 농도에서 무처리군 대비 유의적으로 높은 치사 활성을 보였다. 이상의 결과로부터 녹차 성숙잎으로부터 분리된 효소 처리 조다당 GTE-0은 강력한 면역 활성 증진 효과를 갖고 있음을 결론지을 수 있었다.

Keywords

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