Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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The effect of the cytotoxicity of sodium lauryl sulfate containing toothpaste on HaCaT and NIH-3T3 cells

구강세치제에 함유된 SLS(Sodium lauryl Sulfate)가 HaCaT 세포와 NIH-3T3 세포에 미치는 독성 효과

  • Park, Sang-Rye (Department of Dental Hygiene, Kyungnam College of Information & Technology) ;
  • Kim, Young-Min (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Choi, Byul-Bora (Department of Dental Hygiene, Division of Health Sciences, Dongseo University) ;
  • Kim, Ji-Young (Department of Dental Hygiene, Kyungnam College of Information & Technology)
  • 박상례 (경남정보대학교 치위생과) ;
  • 김영민 (부산대학교 치의학전문대학원 구강해부학교실) ;
  • 최별보라 (동서대학교 치위생학과) ;
  • 김지영 (경남정보대학교 치위생과)
  • Received : 2015.04.24
  • Accepted : 2015.07.30
  • Published : 2015.08.30
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Abstract

Objectives: The purpose of this study was to determine the toxic effects of sodium lauryl sulfate(SLS) in human keratinocyte HaCaT cells and mouse fibroblast NIH-3T3 cells. Methods: The effect of sodium lauryl sulfate(SLS) cell viability and proliferation were determined by WST-1 assay and changes shape of nucleus were evaluated by Hoechst staining under fluorescence microscopy. Additionally, observation of cell morphological changes under light microscopy. Results: SLS induced cytotoxicity and a marked apoptosis in both HaCaT and NIH-3T3 cell lines. With the result of the WST-1 assay, SLS induced the cytotoxicity of 0.005% and 0.0075%, 0.01% SLS for 24 h after HaCaT and NIH-3T3 cells in time and dose-dependent manner(p<0.005). SLS inhibited cell growth and caused apoptosis as evidenced by nuclear fragmentation and condensation. Thus, determination of the morphological changes to define apoptosis was visualized using inverted phase contrast microscopy. Conclusions: SLS had toxicity of the human keratinocyte cells and mouse fibroblast cells and this study will provide the basic data for the development of proper SLS concentration in dentifrice.

Keywords

References

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