Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Influence of Morinda citrifolia (Noni) on Expression of DNA Repair Genes in Cervical Cancer Cells

  • Gupta, Rakesh Kumar (Department of Biochemistry, All India Institute of Medical Sciences) ;
  • Bajpai, Deepti (Department of Biochemistry, All India Institute of Medical Sciences) ;
  • Singh, Neeta (Department of Biochemistry, All India Institute of Medical Sciences)
  • Published : 2015.04.29
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Abstract

Background: Previous studies have suggested that Morinda citrifolia (Noni) has potential to reduce cancer risk. Objective: The purpose of this study was to investigate the effect of Noni, cisplatin, and their combination on DNA repair genes in the SiHa cervical cancer cell line. Materials and Methods: SiHa cells were cultured and treated with 10% Noni, $10{\mu}g/dl$ cisplatin or their combination for 24 hours. Post culturing, the cells were pelleted, RNA extracted, and processed for investigating DNA repair genes by real time PCR. Results: The expression of nucleotide excision repair genes ERCC1, ERCC2, and ERCC4 and base excision repair gene XRCC1 was increased 4 fold, 8.9 fold, 4 fold, and 5.5 fold, respectively, on treatment with Noni as compared to untreated controls (p<0.05). In contrast, expression was found to be decreased 22 fold, 13 fold, 16 fold, and 23 fold on treatment with cisplatin (p<0.05). However, the combination of Noni and cisplatin led to an increase of 2 fold, 1.6 fold, 3 fold, 1.2 fold, respectively (p<0.05). Conclusions: Noni enhanced the expression of DNA repair genes by itself and in combination with cisplatin. However, high expression of DNA repair genes at mRNA level only signifies efficient DNA transcription of the above mentioned genes; further investigations are needed to evaluate the DNA repair protein expression.

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References

  1. Gupta R K, Banerjee A, Pathak S, et al (2013). Induction of mitochondrial-mediated apoptosis by Morinda citrifolia (noni) in human cervical cancer cells. Asian Pac J Cancer Prev, 14, 237-242. https://doi.org/10.7314/APJCP.2013.14.1.237
  2. Rosa MI, Medeiros LR, Rosa DD, et al (2009). Human papillomavirus and cervical neoplasia. Cad Saude Publica, 25, 953-64.
  3. Gupta RK, Singh N (2013). Morinda citrifolia (noni) alter oxidative stress marker and antioxidant activity in cervical cancer cell lines. Asian Pac J Cancer Prev, 14, 4603-6. https://doi.org/10.7314/APJCP.2013.14.8.4603
  4. Su YF, Wu TF, Ko JL, et al (2014). The expression of ribonucleotide reductase m2 in the carcinogenesis of uterine cervix and its relationship with clinicopathological characteristics and prognosis of cancer patients. PLoS One, 9, 91644. https://doi.org/10.1371/journal.pone.0091644
  5. El-Zein RA, Monroy CM, Cortes A, et al (2010). Rapid methods for determination of DNA repair capacity in human peripheral blood lymphocytes amongst smokers. BMC Cancer, 18, 439.
  6. Slyskova J, Korenkova V, Collins AR, et al (2012). Functional, genetic, and epigenetic aspects of base and nucleotide excision repair in colorectal carcinomas. Clin Cancer Res, 18, 5878-87. https://doi.org/10.1158/1078-0432.CCR-12-1380
  7. Min JH, Pavletich NP (2007). Recognition of DNA damage by the Rad4 nucleotide excision repair protein. Nature. 449, 570-5. https://doi.org/10.1038/nature06155
  8. Zhi Y, Yu J, Liu Y, et al (2012). Interaction between polymorphisms of DNA repair genes significantly modulated bladder cancer risk. Int J Med Sci, 9, 498-505. https://doi.org/10.7150/ijms.4799
  9. Mahjabeen I, Ali K, Zhou X, et al (2014). Deregulation of base excision repair gene expression and enhanced proliferation in head and neck squamous cell carcinoma. Tumour Biol. [Epub ahead of print]
  10. Yu H, Liu Z, Huang YJ, et al (2012). Association between Single Nucleotide Polymorphisms in ERCC4 and risk of squamous cell carcinoma of the head and neck. PLoS One, 7, 41853. https://doi.org/10.1371/journal.pone.0041853
  11. Bai ZL, Wang YY, Zhe H, et al (2012). ERCC1 mRNA levels can predict the response to cisplatin-based concurrent chemoradiotherapy of locally advanced cervical squamous cell carcinoma. Radiat Oncol, 7, 221. https://doi.org/10.1186/1748-717X-7-221
  12. Kumar A, Pant MC, Singh HS, et al (2012). Reduced expression of DNA repair genes (XRCC1, XPD, and OGG1) in squamous cell carcinoma of head and neck in North India. Tumor Biol, 33, 111-9. https://doi.org/10.1007/s13277-011-0253-7
  13. Bajpai D, Banerjee A, Pathak S, et al (2013). Decreased expressions of DNA repair genes (XRCC1, ERCC1, ERCC2, and ERCC4) in squamous intraepithelial lesion and invasive squamous cell carcinoma of the cervix. Mol Cell Biochem, 377, 45-53. https://doi.org/10.1007/s11010-013-1569-y
  14. Wang MY, Nowicki D, Anderson G, et al (2008). Liver protective effects of morinda citrifolia (noni). Plant Foods Hum Nutr, 63, 59-63. https://doi.org/10.1007/s11130-008-0070-3
  15. Wang MY, Lutfiyya MN, Weidenbacher-Hoper V, et al (2009). Antioxidant activity of noni juice in heavy smokers. Chem Cent J, 3, 13. https://doi.org/10.1186/1752-153X-3-13
  16. Westendorf J, Effenberger K, Iznaguen H, et al (2007). Toxicological and analytical investigations of Noni (Morinda citrifolia) fruit juice. J Agric Food Chem, 55, 529-37. https://doi.org/10.1021/jf062130i
  17. Clafshenkel WP, King TL, Kotlarczyk MP, et al (2012). Morinda citrifolia (Noni) juice augments mammary gland differentiation and reduces mammary tumor growth in mice expressing the unactivated c-erbB2 Transgene. Evid Based Complement Alternat Med, 2012, 487423.
  18. Li Y, Liu F, Tan SQ, et al (2012). X-Ray repair crosscomplementing group 1 (XRCC1) genetic polymorphisms and cervical cancer risk: a huge systematic review and meta-analysis. PLoS One, 7, 44441. https://doi.org/10.1371/journal.pone.0044441
  19. Wei Q, Wang LE, Sturgis EM, et al (2005). Expression of nucleotide excision repair proteins in lymphocytes as a marker of susceptibility to squamous cell carcinomas of the head and neck. Cancer Epidemiol Biomarkers Prev, 14, 1961-66. https://doi.org/10.1158/1055-9965.EPI-05-0101
  20. Bergs JW, Haveman J, Ten Cate R, et al (2007). Effect of 41 degrees C and 43 degrees C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin. Oncol Rep, 18, 219-26.
  21. Tanida S, Mizoshita T, Ozeki K, et al (2012). Mechanisms of cisplatin-induced apoptosis and of cisplatin sensitivity:potential of bin1 to act as a potent predictor of cisplatin sensitivity in gastric cancer treatment. Int J Surg Oncol, 2012, 862879.
  22. Rabik CA, Dolan ME (2007). Molecular mechanisms of resistance and toxicity associated with platinating agents. Cancer Treat Rev, 33, 9-23. https://doi.org/10.1016/j.ctrv.2006.09.006
  23. Ni M, Zhang WZ, Qiu JR, et al (2014). Association of ERCC1 and ERCC2 polymorphisms with colorectal cancer risk in a Chinese population. Sci Rep, 4, 4112.
  24. Olaussen KA, Mountzios G, Soria JC (2007). ERCC1 as a risk stratifier in platinum-based chemotherapy for nonsmall-cell lung cancer. Curr Opin Pulm Med, 13, 284-289. https://doi.org/10.1097/MCP.0b013e32816b5c63
  25. Bellmunt J, Paz-Ares L, Cuello M, et al (2007). Gene expression of ERCC1 as a novel prognostic marker in advanced bladder cancer patients receiving cisplatin-based chemotherapy. Ann Oncol, 18, 522-8.
  26. Guarrera S, Sacerdote C, Fiorini L, et al (2007). Expression of DNA repair and metabolic genes in response to a flavonoidrich diet. Br J Nutr, 98, 525-533. https://doi.org/10.1017/S0007114507725151
  27. Choi DY, Lee YJ, Hong JT, et al (2012). Antioxidant properties of natural polyphenols and their therapeutic potentials for Alzheimer's disease. Brain Res Bull, 87, 144-153. https://doi.org/10.1016/j.brainresbull.2011.11.014
  28. Astley SB, Elliott RM, Archer DB, et al (2004). Evidence that dietary supplementation with carotenoids and carotenoidrich foods modulate the DNA damage: repair balance in human lymphocytes. Br J Nutr, 91, 63-72. https://doi.org/10.1079/BJN20031001
  29. Katiyar SK (2011). Green tea prevents non-melanoma skin cancer by enhancing DNA repair. Arch Biochem Biophys, 508, 152-8. https://doi.org/10.1016/j.abb.2010.11.015
  30. Moller P, Vogel U, Pedersen A, et al (2003). No effect of 600 grams fruit and vegetables per day on oxidative DNA damage and repair in healthy nonsmokers. Cancer Epidemiol Biomarkers Prev, 12, 1016-22.