Asian Pacific Journal of Cancer Prevention

  • 제16권7호
  • /
  • Pages.2813-2818
  • /
  • 2015
  • /
  • 1513-7368(pISSN)
  • /
  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effect of Curcumin on Invasion of Skin Squamous Cell Carcinoma A431 Cells

  • Wu, Jian (Department of Laboratory Medicine, The First People's Hospital of Yancheng City) ;
  • Lu, Wen-Ying (Pathological Science Laboratory, The Sixth People's Hospital of Yancheng City) ;
  • Cui, Lei-Lei (Department of Laboratory Medicine, The First People's Hospital of Yancheng City)
  • 발행 : 2015.04.14
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: To investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as possible mechanisms of curcumin in prevention and treatment of skin squamous cell carcinoma. Materials and Methods: Highly invasive A431 cells were treated with curcumin at various doses .The cytotoxic effects of treatment with 5, 10, 15, 20, 25, 30, 35, 40 and 50 umol/L curcumin for 24, 48 and 72 hours on A431 cells were measured by MTT assay. The invasion capacity of cells treated with 5, 10 and 15 umol/L curcumin was measured by Transwell test, while adhesive ability was assessed by cell adhesion assay. The effects of 5,10 and 15 umol/L curcumin on expression levels of STAT3 were determined by Western blotting and on transcription levels of STAT3 mRNA by RT-PCR. Results: Treatment with curcumin at a doses of more than 15 umol/L for more than 24 hour inhibited the growth of A431 cells in a time-and dose-dependent fashion (p<0.001). The doses of 15 umol/L and less for 24 hours showed no significant cytotoxic effects on the cells, survival rates being more than 85%.The invasion and adhesive abilities decreased gradually with the increasing curcumin concentration, 15 umol/L exerting the strongest inhibitory effects (p<0.05). Curcumin showed significant dose-dependent inhibitory effects on the transcription level of STAT3 mRNA (p<0.05). Conclusions: Curcumin may reduce the invasive ability of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene in the pathway.

키워드

참고문헌

  1. Aparicio-Siegmund S, Sommer J, Monhasery N, et al (2014). Inhibition of protein kinase II (CK2) prevents induced signal transducer and activator of transcription (STAT) 1/3 and constitutive STAT3 activation. Oncotarget, 5, 2131-48. https://doi.org/10.18632/oncotarget.1852
  2. Chen J, Chi M, Chen C, et al (2013). Obesity and melanoma: exploring molecular links. J Cell Biochem, 114, 1955-61. https://doi.org/10.1002/jcb.24549
  3. Chung SS, Vadgama JV, et al (2015). Curcumin and epigallocatechin gallate inhibit the cancer stem cell phenotype via down-regulation of STAT3-$NF{\kappa}B$ signaling. Anticancer Res, 35, 39-46.
  4. Dai XZ, Yin HT, Sun LF, et al (2013). Potential therapeutic efficacy of curcumin in liver cancer. Asian Pac J Cancer Prev, 14, 3855-9. https://doi.org/10.7314/APJCP.2013.14.6.3855
  5. Fiala M(2015). Curcumin and omega-3 fatty acids enhance NK cell-induced apoptosis of pancreatic cancer cells but curcumin inhibits interferon-${\gamma}$ production: benefits of omega-3 with curcumin against cancer. Molecules, 20, 3020-6. https://doi.org/10.3390/molecules20023020
  6. Hu A, Huang JJ, Jin XJ, et al (2014). Curcumin suppresses invasiveness and vasculogenic mimicry of squamous cell carcinoma of the larynx through the inhibition of JAK-2/STAT-3 signaling pathway. Am J Cancer Res, 5, 278-88.
  7. Jian Wu, Jin-Rong Zhang, Jie Qin, et al (2014). Methylation of E-cadherin and p14ARF gene promoters. Int J Clin Exp Med, 7, 1808-12.
  8. Kang JH, Kang HS, Kim IK, et al (2015). Curcumin sensitizes human lung cancer cells to apoptosis and metastasis synergistically combined with carboplatin. Exp Biol Med (Maywood).
  9. Kim GT, Lee SH, Kim YM, et al (2013). Quercetin regulates sestrin 2-AMPK-mTOR Signaling pathway and induces apoptosis via increased intracellular ROS in HCT116 colon cancer cells. J Cancer Prev, 18, 264-70. https://doi.org/10.15430/JCP.2013.18.3.264
  10. Kraljik N, Rosso M, Ageel A, et al (2014). The incidence of skin squamous cell carcinoma in Osijek-Baranja County--an epidemiological study. Coll Antropol, 35, 77-80.
  11. Liu L, Gaboriaud N, Vougogianopoulou K, et al (2014). MLS-2384, a new 6-bromoindirubin derivative with dual JAK/Src kinase inhibitory activity, suppresses growth of diverse cancer cells. Cancer Biol Ther, 15, 178-84 https://doi.org/10.4161/cbt.26721
  12. Talbot JJ, Song X, Wang X, et al (2014). The cleaved cytoplasmic tail of polycystin-1 regulates Src-dependent STAT3 activation. J Am Soc Nephrol, 25, 1737-48. https://doi.org/10.1681/ASN.2013091026
  13. Qian YD, Xu X, Wang L, et al (2014).Clinical safety of chemotherapy for elderly cancer patients complicated with hypertension. Asian Pac J Cancer Prev, 15, 9875-7. https://doi.org/10.7314/APJCP.2014.15.22.9875
  14. Qu Y, Dang S, Hou P, et al (2013). Gene methylation in gastric cancer. Clin Chim Acta, 23, 53-65.
  15. Snyder M, Huang J, Huang XY, et al (2014). A STAT3/$NF{\kappa}B$ complex is necessary for the expression of fascin in metastatic breast cancer cells in response to IL-6 and TNF-${\alpha}$. J Biol Chem, 11, 30082-9.
  16. Toden S, Okugawa Y, Buhrmann C, et al (2015). Novel evidence for curcumin and boswellic acid induced chemoprevention through regulation of miR-34a and miR-27a in colorectal cancer. Cancer Prev Res. [Epub ahead of print]
  17. Uehara Y, Inoue M, Fukuda K, et al (2014). Inhibition of ${\beta}$-catenin and STAT3 with a curcumin analog suppresses gastric carcinogenesis in vivo. Gastric Cancer. [Epub ahead of print]
  18. Wang J, Zhang L, Chen G, et al (2014). Small molecule 1'-acetoxychavicol acetate suppresses breast tumor metastasis by regulating the SHP-1/STAT3/MMPs signaling pathway. Breast Cancer Res Treat. [Epub ahead of print]
  19. Xu W, Yang Z, Zhou SF, et al (2014). Posttranslational regulation of phosphatase and tensin homolog (PTEN) and its functional impact on cancer behaviors, Drug Des Devel Ther, 8, 1745-51.
  20. Zhou GZ, Sun GC, Zhang SN, et al (2015). The interplay between autophagy and apoptosis induced by one synthetic curcumin derivative hydrazinobenzoylcurcumin in A549 lung cancer cells. J Biochem Mol Toxicol. [Epub ahead of print]

피인용 문헌

  1. Use of Polyphenolic Compounds in Dermatologic Oncology vol.17, pp.4, 2016, https://doi.org/10.1007/s40257-016-0193-5
  2. The effect of wool hydrolysates on squamous cell carcinoma cells in vitro. Possible implications for cancer treatment vol.12, pp.8, 2017, https://doi.org/10.1371/journal.pone.0184034
  3. Anticancer Activity of Curcumin and Its Analogues: Preclinical and Clinical Studies vol.35, pp.1, 2017, https://doi.org/10.1080/07357907.2016.1247166
  4. Curcumin: the spicy modulator of breast carcinogenesis vol.36, pp.1, 2017, https://doi.org/10.1186/s13046-017-0566-5
  5. Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells vol.37, pp.6, 2017, https://doi.org/10.3892/or.2017.5637
  6. Synergistic anticancer effect of curcumin and chemotherapy regimen FP in human gastric cancer MGC-803 cells vol.14, pp.3, 2017, https://doi.org/10.3892/ol.2017.6627
  7. Correlation between tripartite motif-containing protein 44 protein expression and the prognosis of postoperative patients exhibiting skin squamous cell carcinoma vol.97, pp.44, 2018, https://doi.org/10.1097/MD.0000000000013021
  8. Gadd45a gene silencing by RNAi promotes cell proliferation and inhibits apoptosis and senescence in skin squamous cell carcinoma through the p53 signaling pathway vol.233, pp.9, 2018, https://doi.org/10.1002/jcp.26588
  9. Curcumin as a therapeutic agent in leukemia pp.00219541, 2019, https://doi.org/10.1002/jcp.28072
  10. Cellular and molecular mechanisms of curcumin in prevention and treatment of disease pp.1549-7852, 2019, https://doi.org/10.1080/10408398.2018.1552244