Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Citrus Ethanol Extracts Promotes Innate Immune Response by Activating NF-κB

유자 에탄올 추출물의 면역력 증진 효과

  • Received : 2015.04.30
  • Accepted : 2015.08.13
  • Published : 2015.09.30
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Abstract

Citrus junos Sieb. ex Tanaka has been traditionally called Yuza in Korea and is used as a cuisine material or tea as well as medicinal herb. In this study, we evaluated the immune-enhancing effect of Citrus junos ethanol extract (CJE) on RAW264.7 mouse macrophage and primary immunocytes. CJE treatment showed increased macrophage activity in a dose-dependant manner. CJE also enhanced natural killer (NK) cell activity. We measured lactate dehydrogenase (LDH) level as a measurement of NK cell cytotoxicity against YAC-1 lymphoma cells. CJE treatment showed an increased LDH level in a dose-dependent manner. Finally, we evaluated the effect of CJE on mouse primary splenocyte proliferation. CJE treatment slightly increased splenocyte proliferation compared to the control. The results of this study suggest that CJE can help immune function via macrophage cytokine production, increased NK cell activity, and splenocyte proliferation.

본 연구에서는 유자가 선천성 면역력에 미치는 효과를 알아보기 위하여 유자 30% 주정추출물(CJE)을 사용하였으며 선천성 면역에 중요한 역할을 하는 대식세포를 이용해 실험하였다. CJE는 마우스 대식세포인 RAW264.7에서 $1,000{\mu}g/mL$의 최고 농도까지 세포독성을 보이지 않았고, 전사인자 $NF-{\kappa}B$와 염증성 매개물질인 COX-2, PGE2의 활성 및 발 현 증강에 영향을 미치며, 특히 $300{\mu}g/mL$의 농도에서부터 유의적 차이를 보이는 것으로 확인되었다. 산화질소 생성능과 대식세포에서 분비되는 사이토카인인 $TNF-{\alpha}$, $IL-1{\beta}$의 발현을 대조군에 비해 농도 의존적으로 증가시킨다는 결과를 얻었으나, IL-6에서는 통계적으로 약간의 유의성이 있는 증가를 보였고 IL-10은 정상대조군에 비해 거의 유의적인 차이를 보이지 않았다. CJE는 또한 NK 세포의 활성을 농도 의존적으로 증가시키고 비장세포의 증식능도 농도 의존적으로 증가시킨다는 것을 확인하였다. 이러한 결과로 미루어 보아 CJE는 인체의 대식세포 활성의 증가를 통해 선천성 면역력을 증가시킬 것으로 판단된다.

Keywords

References

  1. Yu AR, Park HY, Kim YS, Ha SK, Hong HD, Choi HD. 2012. Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J Korean Soc Food Sci Nutr 41: 1671-1676. https://doi.org/10.3746/jkfn.2012.41.12.1671
  2. Uthaisangsook S, Day NK, Bahna SL, Good RA, Haraguchi S. 2002. Innate immunity and its role against infections. Ann Allergy Asthma Immunol 88: 253-264. https://doi.org/10.1016/S1081-1206(10)62005-4
  3. Birk RW, Gratchev A, Hakiy N, Politz O, Schledzewski K, Guillot P, Orfanos CE, Goerdt S. 2001. Alternative activation of antigen-presenting cells: concepts and clinical relevance. Hautarzt 52: 193-200. https://doi.org/10.1007/s001050051289
  4. Yoo SA, Kim OK, Nam DE, Kim Y, Baek H, Jun W, Lee J. 2014. Immunomodulatory effects of fermented Curcuma longa L. extracts on RAW 264.7 cells. J Korean Soc Food Sci Nutr 43: 216-223. https://doi.org/10.3746/jkfn.2014.43.2.216
  5. Yu AR, Park HY, Choi IW, Park YK, Hong HD, Choi HD. 2012. Immune enhancing effect of medicinal herb extracts on a RAW 264.7 macrophage cell line. J Korean Soc Food Sci Nutr 41: 1521-1527. https://doi.org/10.3746/jkfn.2012.41.11.1521
  6. Byun MW. 2013. Immunomodulatory activities of apple seed extracts on macrophage. J Korean Soc Food Sci Nutr 42: 1513-1517. https://doi.org/10.3746/jkfn.2013.42.9.1513
  7. Miyazawa N, Tomita N, Kurobayashi Y, Nakanishi A, Ohkubo Y, Maeda T, Fujita A. 2009. Novel character impact compounds in yuzu (Citrus junos Sieb. ex Tanaka) peel oil. J Agric Food Chem 57: 1990-1996. https://doi.org/10.1021/jf803257x
  8. Jin TY, Wang MH, Yin Y, Eun JB. 2008. Effect of Citrus junos peel on the quality and antioxidant activity of traditional rice wine, Jinyangju. J Korean Soc Food Sci Nutr 37: 76-82. https://doi.org/10.3746/jkfn.2008.37.1.76
  9. Kim DH, Chung JH, Yoon JS, Ha YM, Bae S, Lee EK, Jung KJ, Kim MS, Kim YJ, Kim MK. 2013. Ginsenoside Rd inhibits the expressions of iNOS and COX-2 by suppressing NF-${\kappa}B$ in LPS-stimulated RAW264.7 cells and mouse liver. J Ginseng Res 37: 54-63. https://doi.org/10.5142/jgr.2013.37.54
  10. Kim AJ, Kim YO, Shim JS, Hwang JK. 2007. Immunostimulating activity of crude polysaccharide extract isolated from Curcuma xanthorrhiza Roxb. Biosci Biotechnol Biochem 71: 1428-1438. https://doi.org/10.1271/bbb.60241
  11. Kang BK, Kim KBWR, Ahn NK, Choi YU, Kim MJ, Bark SW, Pak WM, Kim BR, Park JH, Bae NY, Ahn DH. 2014. Immuno-stimulating activities of skipjack tuna Katsuwonus pelamis cooking juice concentrates on mouse macrophages and spleen cells. Kor J Fish Aquat Sci 47: 776-784.
  12. Lowenstein CJ, Snyder SH. 1992. Nitric oxide, a novel biologic messenger. Cell 70: 705-707. https://doi.org/10.1016/0092-8674(92)90301-R
  13. Kim JJ, Lee SW, Park KW, Seo KI, Yee ST. 2012. Effect of Flammulina velutipes extracts cultivated with oriental herbal plants on the activation of immune cells. J Life Sci 22: 828-836. https://doi.org/10.5352/JLS.2012.22.6.828
  14. Biron CA, Nguyen KB, Pien GC, Cousens LP, Salazar- Mather TP. 1999. Natural killer cells in antiviral defense: function and regulation by innate cytokines. Annu Rev Immunol 17: 189-220. https://doi.org/10.1146/annurev.immunol.17.1.189
  15. Frese-Schaper M, Keil A, Yagita H, Steiner SK, Falk W, Schmid RA, Frese S. 2014. Influence of natural killer cells and perforin-mediated cytolysis on the development of chemically induced lung cancer in A/J mice. Cancer Immunol Immunother 63: 571-580. https://doi.org/10.1007/s00262-014-1535-x
  16. Ha Y, Kim OK, Nam DE, Kim Y, Kim E, Jun W, Lee J. 2015. Effect of Curcuma longa L. extracts on natural killer cells and T cells. J Korean Soc Food Sci Nutr 44: 307-313. https://doi.org/10.3746/jkfn.2015.44.3.307
  17. Ryu DS, Kim SH, Lee DS. 2008. Immunomodulating activity of Salicornia herbacea extract. Kor J Microbiol Biotechnol 36: 135-141.
  18. Yoo KM, Hwang IK, Park JH, Moon B. 2009. Major phytochemical composition of 3 native Korean citrus varieties and bioactive activity on V79-4 cells induced by oxidative stress. J Food Sci 74: C462-C468. https://doi.org/10.1111/j.1750-3841.2009.01229.x
  19. Hirota R, Roger NN, Nakamura H, Song HS, Sawamura M, Suganuma N. 2010. Anti-inflammatory effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils. J Food Sci 75: H87-92. https://doi.org/10.1111/j.1750-3841.2010.01541.x
  20. Seo YS, Shin KS. 2012. Immune system-stimulating activities of mucilage polysaccharides isolated from Opuntia humifusa. J Korean Soc Food Sci Nutr 41: 95-102. https://doi.org/10.3746/jkfn.2012.41.1.095

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