Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Sinigrin content of different parts of Dolsan leaf mustard

돌산갓의 부위별 sinigrin 함량

  • Oh, SunKyung (Department of Biomedical Electronic and Engineering, Chonnam National University) ;
  • Kim, KiWoong (Department of Marine Bio Food Science, Chonnam National University) ;
  • Bae, SangOk (Department of Culinary Art, Chodang University) ;
  • Choi, Myeong Rak (Department of Biomedical Electronic and Engineering, Chonnam National University)
  • 오선경 (전남대학교 바이오전자 메디컬공학 협동과정) ;
  • 김기웅 (전남대학교 해양바이오 식품학과) ;
  • 배상옥 (초당대학교 조리과학부) ;
  • 최명락 (전남대학교 바이오전자 메디컬공학 협동과정)
  • Received : 2015.05.22
  • Accepted : 2015.08.18
  • Published : 2015.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aims of this study was to optimize the extraction conditions of sinigrin from Dolsan leaf mustard. Dolsan leaf mustard (Dolsan-eup, Yeosu-si) harvested during at May 2014 was used for sinigrin extraction. After the extraction of sinigrin using 50% $CH_3CN$, 10% $NH_4Cl$, 60% $CH_2OH$, and 70% $CH_3OH$, the sinigrin content was measured by HPLC analysis. The results showed that sinigrin content was highest with 50% $CH_3CN$ solvent extraction and UV detector sensitivity was greater at 228 nm rather than at 242 nm. The sinigrin concentrations of leaf, stem and root with 50% $CH_3CN$ extraction were 345 ppm, 728 ppm, and 539 ppm, respectively. After extraction of the different parts of Dolsan leaf mustard, The standard retention time by HPLC analysis of sinigrin content was 2.054, 2.032, 2.059, and 2.035 min from the root, stalk, and leaf, respectively. On the other hand, HPLC analysis showed that the leaf extracts contained glucoraphanin, one of glucosinolates. The optimum time and extraction solvent for the sinigrin extraction from Dolsan leaf mustard was found to be 24 hr with 50% $CH_3CN$ solvent. In addition, opotimum UV detector k at 228 nm. These results showed that the optimum extraction conditions for Dolsan leaf mustard were 24 hr extraction with 50% $CH_3CN$ solvent. In addition, the optimum wavelength of UV detector was determined to be 228 nm for sinigrin analysis. Therefore, this study could provide a useful information for sinigrin extraction and its systematic analysis during the storage.

돌산갓에서의 glucosinolates인 sinigrin의 분석법을 확립하기 위해 다양한 용매를 이용하여 추출 후 정성 정량하였다. 돌산갓을 뿌리, 줄기, 잎으로 구분하여 50% $CH_3CN$, 10% $NH_4Cl$, 60% $CH_2OH$, 70% $CH_3OH$ 을 이용해 추출시 50% $CH_3CN$이 가장 sinigrin 함량이 높게 나타났으며 나머지 용매들은 큰 차이가 없었다. 또한 갓 부위별에서 sinigrin함량은 큰 차이가 없었으며 줄기 부위의 50% $CH_3CN$추출물의 sinigrin함량이 728 ppm으로 다소 높게 나타났다. Sinigrin은 농도별로 standard curve를 작성하여 HPLC에 의한 돌산갓 부위별 sinigrin 함량을 정량화 하였고 HPLC로 분석시 UV detector 감도는 242 nm보다 228 nm에서 높게 나타났으며 228 nm에서는 줄기, 뿌리, 잎 순서로 sinigrin함량이 나타났다. 50% $CH_3CN$로 추출한 돌산갓의 chromatogram은 표준물질인 sinigrin의 retention time과 일치하였으며, 잎에서 나타난 peak는 glucosinolates인 glucoraphanin으로 확인되었다. 본 연구의 결과로부터 돌산갓에 함유된 sinigrin 분석을 위한 추출용매는 50% $CH_3CN$, UV detector 파장은 228 nm가 가장 적합하다는 것을 알 수 있었다. 또한, sinigrin의 추출 및 분석이 체계화됨으로써 돌산갓을 이용한 다양한 식품의 저장기간에 따른 sinigrin 함량변화 및 생리기능에 관한 연구에 활용 될 수 있을 것으로 판단된다.

Keywords

References

  1. Popova IE, Morra MJ (2014) Simultaneous quantification of sinigrin, sinalbin, and anionic glucosinolate hydrolysis products in Brassica juncea and Sinapis alba seed extracts using ion chromatography. J Agric Food Chem, 65, 10687-10693
  2. Cho YS, Ha BS, Park SK, Chun SS (1993) Contents of carotenoids and chlorophyll in Dolsan leaf mustard (Brassica juncea). Korean J Dietary Culture 8, 153-157
  3. Lee SM, Rhee SH (1997) Inhibitory effects of various cruciferous vegetables on the growth of human cancer cells. J Life Sci, 7, 234-240
  4. Kim H, Kim JY, Kim HJ, Kim DK, Jo HJ, Han BS, Kim HW, Kim JB (2011) Anticancer activity and quantitative analysis of glucosinolates from green and red leaf mustard. J Korean Soc Food Nutr, 24, 362-366 https://doi.org/10.9799/ksfan.2011.24.3.362
  5. Kim JO, Kim MN, Park KY, Moon SH, Ha YL (1993) Antimutagenic effects of 4-decanol identified from mustard leaf. J Korean Agric Chem Soc, 36, 424-427
  6. Cho YS, Park SK, Chun SS, Park JS (1993) Analysis of isothiocyanates in Dolsan leaf mustard (Brassica juncea). Korean J Dietary Culture, 8, 147-151
  7. Choi MR, Yoo EJ, Song SH, Kang DS, Park JC, Lim HS (2001) Comparison of physiological activity in different parts of Dolsan leaf mustard. J Korean Soc Food Sci Nutr, 30, 721-725
  8. Yoo EJ, Choi MR, Lim HS (2004) The relationship between ACE inhibitory activity and degradations of sulfur containing materials in Dolsan leaf mustard juice. Biotechnol Bioprocess Eng, 9, 400-404 https://doi.org/10.1007/BF02933065
  9. Lim HS, Yoo E J, Choi MR (2000) Changes of physiological activity according to fermentation period of mustard leaf Kimchi. J Microbiol Biotechnol, 10, 43-47
  10. Choi YY, Yoo EJ, Lim HS, Kang DS, Nishizawa N Choi MR (2001) The relationship between physiological activity and cell number in Dolsan leaf mustard Kimchi (Brassica juncea). J Food Sci Nutr, 6, 117-121
  11. Choi MR, Yoo E J, Lim HS (2003) Effect of lactic acid bacterium on antioxidative and ACE inhibitory activity in Dolsan leaf mustard Kimchi. Korean J Life Sci, 13, 59-66 https://doi.org/10.5352/JLS.2003.13.1.059
  12. Yoo EJ, Lim HS, Park KO, Choi MR (2005) Cytotoxic, antioxidative, and ACE inhibiting activities of Dolsan leaf mustard juice (DLMJ) treated with lactic acid bacteria. Biotechnol Bioprocess Eng, 10, 60-66 https://doi.org/10.1007/BF02931184
  13. Lim HS, Park KO, Nishizawa N, Bae SO Choi MR (2008) Cytotoxicity of extracts from Dolsan leaf mustard Kimchi treated with lactic acid bacteria on lung and gastric cancer cells. Biotechnol Bioprocess Eng, 13, 174-181 https://doi.org/10.1007/s12257-007-0127-4
  14. Lim HS (2002) The study for contents of sinigrin in Dolsan leaf mustard Kimchi during fermentation periods. J Life Sci, 12, 523-527 https://doi.org/10.5352/JLS.2002.12.5.523
  15. Tao CC, Brian BH (2004) Isolation of intact glucosinolates from mustard seed meal to increase the sustainability of biodiesel utilization. An ASAE/CSAE Meeting Presentation, 040679
  16. Tsao R, Yu Q, Potter J, Chiba M (2002) Direct and simultaneous analysis of sinigrin and allylIsothiocyanate in mustad samples by high-performance liquid chromatography. J Agric Food Chem, 50, 4749-4753 https://doi.org/10.1021/jf0200523
  17. Rungapamestry V, Duncan AJ, Fuller Z, Ratcliffe B (2006) Changes in glucosinolate concentrations, myrosinase activity, and production of metabolites of glucosinolates in cabbage (Brassica oleracea Var. capitata) cooked for different durations. J Agric Food Chem, 54, 7628-7634 https://doi.org/10.1021/jf0607314
  18. Rangkadilok N, Nicolas ME, Bennett RN, Premier, Robert RR, Eagling DR, Taylor PWJ (2002) Developmental changes of sinigrin and glucoraphanin in three Brassica species (Brassica nigra, Brassica juncea and Brassica oleracea var. italica). J Sci Hort, 96, 11-26 https://doi.org/10.1016/S0304-4238(02)00118-8
  19. Rangkadilok N, Nicolas ME, Bennett RN, Premier, Robert RR, Eagling DR, Taylor PWJ (2002) Determination of sinigrin and glucoraphanin in Brassica species using a simple extraction method combined with ion-pair HPLC analysis. J Sci Hort, 96, 27-41 https://doi.org/10.1016/S0304-4238(02)00119-X
  20. Kang SK (1995) Isolation and antimicrobial activity of antimicrobial substance obtained from leaf mustard (Brassica juncea). J Korean Soc Food Nutr, 24, 695-701
  21. Lim HS (2002) The study for contents of sinigrin in Dolsan leaf mustard kimchi during fermentation periods. J Life Sci, 12, 523-527 https://doi.org/10.5352/JLS.2002.12.5.523
  22. Shin CS, Seo KI, Kang KS, Ahn CW, Kim YG, Shim KH (1996) Purification and enzymatic properties of myrosinase in Korean mustard seed (Brassica juncea). J Korean Soc Food Nutr, 25, 687-694
  23. Park JR, Park SK, Cho YS, Chun SS (1994) Purification and characterization of myrosinase in Dolsan mustard (Brassica juncea) and changes in myrosinase activity during fermentation of leaf mustard Kimchi. Korean J Dietary Culture, 9, 137-142

Cited by

  1. 정선 갓의 영양성분 및 세포기반 항산화 활성 분석 vol.32, pp.5, 2019, https://doi.org/10.9799/ksfan.2019.32.5.462
  2. Antioxidant Activity, Whitening and Anti-wrinkle Effects of Leaf and Seed Extracts of Brassica juncea L. Czern. vol.18, pp.3, 2015, https://doi.org/10.20402/ajbc.2020.0038
  3. Antiobesity effect of Brassica juncea cultivated in Jeongseon with optimized sinigrin content using 3T3‐L1 adipocytes vol.45, pp.4, 2015, https://doi.org/10.1111/jfbc.13650