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Antioxidant and anti-fatigue effects of abalone (Haliotis discus hannai) composites containing natural plants

전복 복합물의 항산화능 및 피로개선 효과

  • Received : 2015.05.20
  • Accepted : 2015.07.10
  • Published : 2015.08.30

Abstract

To develop a functional drink using abalone, two abalone composites (APM-1, APM-2) were prepared by mixing the abalone and natural plants (Lycii fructus and Rubus coreanus Miq.). Their antioxidant and anti-fatigue effects were evaluated using rats running on a treadmill after 4 weeks supplementation of the abalone composites. Experimental groups were divided into four groups including normal (non-exercised group), control (exercised group), one dose per day (EAPM-1), and three doses per day (EAPM-2) with exercise by running. Antioxidant activities, and total phenols and flavonoids contents of APM-2 were significantly higher than those of APM-1 (p<0.05). Total lipid content in serum of EAPM-2 was significantly lower than that of control group (p<0.05). Lactate dehydrogenase activity and blood urea nitrogen content of EAPM-1 and EAPM-2 groups were significantly lower than that of control group. There were no significant difference in aspartate aminotransferase activity among control, EAPM-1 and EAPM-2 groups. In the meanwhile, alanine aminotransferase and alkaline phosphatase activities were significantly lower than that of control group. In serum and liver tissues of EAPM-1 and EAPM-2 groups, lipid peroxide contents significantly decreased compared to control group. DPPH radical scavenging activities in liver tissues of EAPM-1 and EAPM-2 groups were significantly higher than those of control group. Therefore, abalone composites were effective for the alleviation of oxidative stress caused by treadmill running, which was dependent on antioxidant activity and phenolic compounds content.

전복 음료를 개발하기 위하여 전복에 식물류를 혼합한 전복 복합물의 in vitro 항산화능을 측정하였으며, 정상군, 대조군, 전복 복합물의 성인 1일 1회분(APM-1) 급이군(EAPM-1) 및 3회분(APM-2) 급이군(EAPM-2)으로 구분하여 4주간 실험사육하였다. 전복 복합물의in vitro 항산화능과 총 페놀 및 플라보노이드 함량은 APM-2가 유의적으로 높았다. 혈중 총 지질 함량은 대조군에 비해 운동과 전복복합물을 병행급이한 EAPM-2군이 유의적으로 감소되었다. LDH 활성 및 BUN 함량은 대조군에 비해 전복 복합물 급이군에서 유의적으로 감소되었다. AST 활성은 전복 복합물 급이 시 대조군과 유의차가 없었으며, ALT 및 ALP 활성은 대조군에 비해 유의적으로 감소되었다. 혈청 및 간 조직에서 지질과산화물 함량은 대조군에 비해 전복 복합물 급이 군에서 유의적으로 감소하였다. 간 조직의 라디칼 소거능은 대조군에 비해 전복 복합물의 급이 시 유의적으로 증가하였다. 따라서 전복 복합물은 항산화능 및 페놀 화합물에 의존하여 트레드밀 운동에 의한 산화적 스트레스 해소 및 피로 회복 에 도움이 될 것으로 예상된다.

Keywords

References

  1. Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ (2008) Antioxidant activity of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr, 37, 965-971 https://doi.org/10.3746/jkfn.2008.37.8.965
  2. Kumar CT, Reddy VK, Thyagarajuk PM, Reddanna PP (1992) Dietary supplementation of vitamin E products heart tissue exercise induced oxidant stress. Mol Cell Biochem, 111, 109-115
  3. Kim HL, Kang SG, Kim IC, Kim SJ, Kim DW, Ma SJ, Gao T, Li H, Kim MJ, Lee TH, Ham KS (2006) In vitro anti-hypertensive, antioxidant and anticoagulant activities of extracts from Haliotis discus hannai. J Korean Soc Food Sci Nutr, 35, 835-840 https://doi.org/10.3746/jkfn.2006.35.7.835
  4. Koh SM, Kim HS, Cho YC, Kang SG, Kim JM (2009) Preparation and physicochemical characteristics of abalone meat aged in Kochujang. J Korean Soc Food Sci Nutr, 38, 773-779 https://doi.org/10.3746/jkfn.2009.38.6.773
  5. Lim SY (2014) Cytotoxic and antioxidant activities abalone (Haliotos discus hannai) extracts. J Life Sci, 24, 737-742 https://doi.org/10.5352/JLS.2014.24.7.737
  6. Shin JH, Lee SJ, Choi DJ, Kang MJ, Sung NJ (2008) Antioxidant and alcohol dehydrogenase activity of water extracts from abalone containing medicinal plants. Korean J Food Cookery Sci, 24, 182-187
  7. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  8. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTs radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  9. Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power" : the FRAP assay. Anal Biochem, 230, 70-79
  10. Gutfinger T (1981) Polyphenols in olive oil. J Am Oil Chem Soc, 58, 966-968 https://doi.org/10.1007/BF02659771
  11. Moreno MIN, Isla MI, Sanpietro AR, Vattuone MA (2000) Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthnopharmacol, 71, 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  12. Frings CS, Fendley TW, Dunn RT, Queen CA (1972) Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction. Clin Chem, 18, 763-764
  13. Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 18, 499-502
  14. Yagi K (1984) Assay for blood plasma or serum. In : Method in Enzymology, Packer L (Editor), Academic Press, Inc., NY, USA, p 328-331
  15. Uchiyama M, Mihara M (1978) Determination of malondialdehyde precursor in tissues by TBA test. Anal Biochem, 86, 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  16. Lim BO, Seo TW, Shin HM, Park DK, Kim SU, Cho KH, Kim HC (2000) Effect of Betulae platyphyllae cortex on free radical in diabetic rats induced by streptozotocin. Korean J Herbology, 15, 69-77
  17. Cho HS, Lee SJ, Shin JH, Kang MJ, Cho HS, Lee HJ, Sung NJ (2007) Antioxidative activity and nitrite scavenging effect of the composites containing medicinal plant extracts. J Life Sci, 17, 1135-1140 https://doi.org/10.5352/JLS.2007.17.8.1135
  18. Lee JM, Lee SH, Kim HM (2000) Use of Oriental herbs as medical food. Food Ind Nutr, 5, 50-56
  19. Na CS, Kim HK, Kim JB, Roh HJ, Um NN, Noh HJ, Na DS, Dong MS, Hong CY (2013) The effects of Hovenia dulcis fruit hot water extracts on anti-fatigue and improvement of the exercise performance in SD rats. Yakkak Hoeji, 57, 348-356
  20. Kang JR, Lee SJ, Hwang CR, Kim IS, Sung NJ (2013) Effect of black garlic and Gaeddongssuk (Artemisia annua L.) extracts on the lipid profile and hepatic antioxidant enzyme activities of exercised rats. J Korean Soc Food Sci Nutr, 42, 869-876 https://doi.org/10.3746/jkfn.2013.42.6.869
  21. Kang MJ, Shin JH, Sung NJ (2011) Recovery effect of garlic extract and vitamin B group enhanced diet on swimming fatigue. J Life Sci, 21, 875-883 https://doi.org/10.5352/JLS.2011.21.6.875
  22. Na CS, Hong CY, Na DS, Kim JB, Yoon SY, Lee SB, Dong MS (2013) Hot water extract of Hovenia dulcis peduncle improves exercise performance and anti-fatigue effect in rats. Korean J Pharmacogn, 44, 83-90
  23. Kim YJ, Jung UJ, Lee GD, Choi MS (2012) Effects of herbal sports drinks containing Prunus mume fruit extract on the plasma lipid profile and endurance of rats. J Korean Soc Food Sci Nutr, 41, 1409-1416 https://doi.org/10.3746/jkfn.2012.41.10.1409
  24. Lee SJ, Kim IS, Lee HJ, Oh SJ, Shin JH, Kim JG, Sung NJ (2013) Effect of black garlic and herb formulas in lipid profiles and antioxidant status in rats by interval running training. J Life Sci, 23, 1436-1444 https://doi.org/10.5352/JLS.2013.23.12.1436
  25. Song TC, Han D, Lee CH, Kim YE, Jung KA, Kim HY (2005) Effect of dietary supplementation of two tonic formula on the forced-swimming capacity of rats. Korean J Food Sci Technol, 37, 648-655
  26. Agner E, Kelbaek H, Fogh-Anderson N, Morck HI (1988) Coronary and skeletal muscle enzyme change during a 14 km run. Acta Med Scand, 224, 183-186
  27. Kim SM, Ko JH, Shim EJ, Lee DH, Cho DJ, Kim DH, Min KS, Yoo KY (2008) Serum creatinine, blood urea nitrogen change in low birth weight infants during their first days of life. Korean J Perinatol, 19, 181-189
  28. Kang YJ, Kim HJ, Han JS (2011) Effects of Ishige okamurae extract supplement on blood glucose and antioxidant systems in type 2 diabetic patients. J Korean Soc Food Sci Nutr, 40, 1726-1733 https://doi.org/10.3746/jkfn.2011.40.12.1726
  29. Kim YS, Ryu BH, Kim JS (2009) Effect of Wongisaengmaek-san extract on antioxidative and anti-fatigue activity. Korean J Orient Int Med, 30, 94-106
  30. Kwon TD, Ryu SP, Jang WC, Lee SC (2002) Effects of green tea polyphenol ingestion on blood lipids, MDA and SOD in rats. Korean J Exercise Nutr, 6, 85-88
  31. Kim SH, Baek YH (2011) Effects of aerobic exercise and black garlic intake on blood lipids, lipid peroxidation and BAP in rats. J Life Sci, 21, 1025-1031 https://doi.org/10.5352/JLS.2011.21.7.1025
  32. Haenen GR, Paquay JB, Korthouwer RE, Bast A (1997) Peroxynitrite scavenging by flavonoids. Biochem Bioph Res Co, 236, 591-593 https://doi.org/10.1006/bbrc.1997.7016
  33. Zhu BW, Wang LS, Zhou DY, Li DM, Sun LM, Yang JF, Wu HT, Zhou XQ, Tada M (2008) Antioxidant activity of sulphated polysaccharide conjugated from abalone (Haliotos discus hannai Ino). Euro Food Res Technol, 22, 827-832
  34. Jimemez-Escrig A, Goni Cambrodon I (1999) Nutritional evaluation and physiological effects of edible seaweeds. Arch Latinoam Nutr, 49, 114-120
  35. Kwon JW, Lee HK, Park HJ, Song JY (2012) Physiological activities of Rubus coreanus Miq. extracts using different extraction methods. Korean J Food Cookery Sci, 28, 25-32 https://doi.org/10.9724/kfcs.2012.28.1.025
  36. Shon HK, Lee YS, Park YH, Kim MJ, Lee KA (2008) Physico-chemical properties of Gugija (Lyciifructus) extracts. J Food Cookery Sci, 24, 905-911
  37. Hwang EY, Hong JH, Choi JH, Lee EJ, Lee IS (2009) Study on anti-obesity and hypoglycemic effects of Lycium chinense Mill extracts. J Korean Soc Food Sci Nutr, 38, 1528-1534 https://doi.org/10.3746/jkfn.2009.38.11.1528
  38. Jung MA, Cho SH, Lee SY, Kim JH, Oh KN, Kim YS, Yoo GS, Lee DW, Kim SO (2014) Effects of unripe Rubus coreanus Miquel extract on improvement of lipid metabolism in C57BL/6 mice fed a high-cholesterol diet. J Korean Soc Food Sci Nutr, 43, 650-655 https://doi.org/10.3746/jkfn.2014.43.5.650

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