DOI QR코드

DOI QR Code

Neuroanatomical Comparative Studies on the Motor and Sensory Neurons Associated with Cheonji(PC1) in the Rats

흰쥐에서 천지(PC1)와 관련된 운동신경과 감각신경의 분포영역에 대한 신경해부학적 연구

  • Lee, Sun-Ho (Department of Meridian, College of Korean Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Acupoint & Anatomy, College of Korean Medicine, Woosuk University) ;
  • Lee, Sang-Ryong (Department of Meridian, College of Korean Medicine, Woosuk University)
  • 이순호 (우석대학교 한의과대학 경혈학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 이상룡 (우석대학교 한의과대학 경혈학교실)
  • Received : 2015.08.31
  • Accepted : 2015.09.15
  • Published : 2015.09.27

Abstract

This study was performed to comparative investigate the distribution of primary sensory and motor neurons associated with Cheonji(PC1) acupoint by using neural tracing technique. A total 4 SD rats were used in the present study. After anesthesia, the rats received microinjection of $6{\mu}l$ of cholera toxin B subunit(CTB) into the corresponding sites of the acupoints Cheonji(PC1) in the human body for observing the distribution of the related primary sensory neurons in dorsal root ganglia(DRGs) and motor neurons in the spinal cord(C3~T4) and sympathetic ganglia. Three days after the microinjection, the rats were anesthetized and transcardially perfused saline and 4% paraformaldehyde, followed by routine section of the DRGs, sympathetic chain ganglia(SCGs) and spinal cord. Labeled neurons and nerve fibers were detected by immunohistochemical method and observed by light microscope equipped with a digital camera. The labeled neurons were recorded and counted. From this research, the distribution of primary sensory and motor neurons associated with Cheonji(PC1) acupoints were concluded as follows. Muscle meridian related Cheonji(PC1) are controlled by spinal segments of C5~T1, C6~T4, respectively.

Keywords

References

  1. Zhao ZQ. Neural mechanism underlying acupuncture analgesia. Prog Neurobiol. 2008 ; 85(4) : 355-75. https://doi.org/10.1016/j.pneurobio.2008.05.004
  2. Ma SX. Neurobiology of acupuncture: toward CAM. Evid Based Complement Alternat Med. 2004 ; 1(1) : 41-7. https://doi.org/10.1093/ecam/neh017
  3. Ha LJ, Cui JJ, Wang FC, Jing XH, Bai WZ. The expression of calcitonin gene-related peptide in the sensory and motor neurons associated with "Hegu" (LI 4) in the rat. Zhen Ci Yan Jiu. 2014 ; 39(2) : 112-16.
  4. Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH. Neuroanatomical characteristics of acupoint "Chengshan"(BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study. Zhen Ci Yan Jiu. 2010 ; 35(6) : 433-37.
  5. Cui JJ, Ha LJ, Zhu XL, Wang FC, Jing XH, Bai WZ. Specificity of sensory and motor neurons associated with BL40 and GB30 in the rat: a dual fluorescent labeling study. Evid Based Complement Alternat Med. 2013 ; 2013 : 643403.
  6. Wang HF, Shortland P, Park MJ, Grant G. Retrograde and transganglionic transport of horseradish peroxidase-conjugated cholera toxin B subunit, wheatgerm agglutinin and isolectin B4 from Griffonia simplicifolia I in primary afferent neurons innervating the rat urinary bladder. Neuroscience. 1998 ; 87(1) : 275-88. https://doi.org/10.1016/S0306-4522(98)00061-X
  7. Cui JJ, Ha LJ, Zhu XL, Shi H, Wang FC, Jing XH et al. Neuroanatomical basis for acupuncture point PC8 in the rat: neural tracing study with cholera toxin subunit B. Acupunct Med. 2013 ; 31(4) : 389-94. https://doi.org/10.1136/acupmed-2013-010400
  8. Jang I, Cho K, Moon S, Ko C, Lee B, Ko B et al. A study on the central neural pathway of the heart, Nei-Kuan (EH-6) and Shen-Men (He-7) with neural tracer in rats. Am J Chin Med. 2003 ; 31(4) : 591-609. https://doi.org/10.1142/S0192415X03001314
  9. Hirakawa M, McCabe JT, Kawata M. Time-related changes in the labeling pattern of motor and sensory neurons innervating the gastrocnemius muscle, as revealed by the retrograde transport of the cholera toxin B subunit. Cell Tissue Res. 1992 ; 267(3) : 419-27. https://doi.org/10.1007/BF00319364
  10. Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ. Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594. Zhen Ci Yan Jiu. 2011 ; 36(4) : 262-67.
  11. Widenfalk B, Elfvin LG, Wilberg M. Orgin of sympathetic and sensory innervation of the elbow joint in the rat : a retrograde axonal tracing study with wheat germ agglutinin conjugated horseradish peroxidase. J Comp Neurol. 1988 ; 271(3) : 313-18. https://doi.org/10.1002/cne.902710302
  12. Ichikawa H, Helke CJ. Distribution, orgin and plasticity of galanin-immunoreactivity in the rat carotid body. Neuroscience. 1993 ; 52(3) : 757-67. https://doi.org/10.1016/0306-4522(93)90424-E
  13. Kristensson K, Olsson Y. Retrograde transport of horseradish peroxidase in transected axons. II. Relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis. J Neurocytol. 1975 ; 4(6) : 653-61. https://doi.org/10.1007/BF01181628
  14. Scott TM, Drodge KH, Foote J. Peptidergic nerve involvement in the control of endothelium-dependent vascular relaxation. Artery. 1992 ; 19(4) : 211-24.
  15. Hwang YS.. Academic history of Chinese acupuncture. Seoul. pubinmunhwasa. 2005 : 595-607.
  16. Loewy AD, Haxhiu MA. CNS cell groups projecting to pancreatic parasympathetic preganglionic neurons. Brain Res. 1993 ; 620(2) : 323-30. https://doi.org/10.1016/0006-8993(93)90174-L
  17. Joosten EA, Schuitman RL, Vermelis ME, Dederen PJ. Postnatal development of the ipsilateral corticospinal component in rat spinal cord : a light and electron microscopic anterograde HRP study. J Comp Neurol. 1992 ; 326(1) : 133-46. https://doi.org/10.1002/cne.903260112
  18. Strack AM, Loewy AD. Pseudorabies virus : a highly specific transneuronal cell body marker in the sympathetic nervous system. J Neurosci. 1990 ; 10(7) : 2139-147.
  19. Cabot JB, Reiner A, Bogan N : Avian bulbospinal pathways : anterograde and retrograde studies of cells of origin, funicular trajectories and laminar terminations, in Kuypers HGJM, Martin GF(eds) : Descending Pathways to the Spinal Cord, Progress in Brain Research. Amsterdam. Elsevier Bionedical Press. 1982 ; 57 : 79-108.
  20. Kandel E, Schwartz J, Jessell T. Principles of neural science, ed 3. New York. Elsevier. 1991 ; 530-679.
  21. Kritas SK, Pensaert MB. Role of gp63 and gIII of Aujeszky's disease virus in the invasion of the olfactory nervous pathway in neonatal pigs. Acta Vet Hung. 1994 ; 42(2-3) : 309-16.
  22. Kuypers HG, Maisky VA. Retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat. Neurosci Lett. 1975 ; 1(1) : 9-14. https://doi.org/10.1016/0304-3940(75)90004-X
  23. Brodal P, Dietrichs E, Bjaalie JG, Nordby T, Walberg F. Is lectin coupled horseradish peroxidase taken up and transported by damaged as well as by undamaged fibers in the centra nervous system?. Brain Res. 1983 ; 278(1-2) : 1-9. https://doi.org/10.1016/0006-8993(83)90221-4
  24. Holstege JC, Kuypers HG. Brainstem projection to spinal motoneurons : an update. Neuroscience. 1987 ; 23(3) : 809-21. https://doi.org/10.1016/0306-4522(87)90160-6
  25. Li XG, Florence S, Kaas JH. Areal distributions of cortical neurons projecting to different levels of the caudal brain stem and spinal cord in rats. Somatosens Motor Res. 1990 ; 7 : 315-35. https://doi.org/10.3109/08990229009144711
  26. Swett JE, Wikholm RP, Blanks RH, et al. Motoneurons of the rat science nerve. Exp Neurol. 1986 ; 93 : 227-52. https://doi.org/10.1016/0014-4886(86)90161-5
  27. Card JP, Rinaman L, Lynn RB, et al. Pseudorabies virus infection of the rat central nervous system : Ultrastructural characterization of viral replication, transport, and pathogenesis. J Neurosc. 1993 ; 13 : 2515-539.
  28. Card JP, Rinnaman L, Schwaber JS, et al. Neurotropc properties of pseudorabies virus : Uptake and transneuronal passage in the rat central nervous system. J Neurosci. 1990 ; 10(6) : 1976-994.
  29. Yunkyung-Im, Junpyo-Kim, Taehan-Kim. Acupointology. Daejeon. Choracdang. 2008 : 621-27, 637-41, 666-67, 688-89.
  30. Woolf CJ, Fitzgerald M. The properties of neurons recorded in the superficial dorsal horn of the rat spinal cord. J Comp Neurol. 1983 ; 221 : 313-28. https://doi.org/10.1002/cne.902210307
  31. Menetrey D, Chaouch A, Binder D, Besson JM. The origin of the spinomesencephalic tract in the rat: An anatomical study using the retrograde transport of horseradish peroxidase. J Comp Neurol. 1982 ; 206 : 862-67.
  32. Lima D, Coimbra A. Neurons in the substantia gelatinosa rolandi(lamina II) project to the caudal ventrolateral reticular formation of the medulla oblongata in the rat. Neurosci Lett. 1991 ; 132(1) : 16-18. https://doi.org/10.1016/0304-3940(91)90421-O
  33. Giesler GJ, Cannon JT, Urca G, Liebeskind JC. Long ascending projections from substantia gelatinosa rolandi and subjacent dorsal horn in the rat. Science. 1978 ; 202(1) : 984-86. https://doi.org/10.1126/science.715454
  34. Burstein R, Cliffer KD, Giesler GJ, Jr. Direct somatosensory projections from the spinal cord to the hypothalamus and telencephalon. J Neurosci. 1987 ; 7(12) : 4159-164.
  35. Light AR, Kavookjian AM. Morphology and ultrastructure of physiologically identified substantia gelatinosa(lamina II) neurons with axons that terminate in deeper dorsal horn laminae(III-V). J Comp Neurol. 1988 ; 267 : 172-89. https://doi.org/10.1002/cne.902670203
  36. Matsushita M, Hosoya Y. Cells or origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase. Brain Res. 1979 ; 173 : 185-200. https://doi.org/10.1016/0006-8993(79)90620-6

Cited by

  1. Trends of Studies in Korean Journal of Acupuncture vol.33, pp.1, 2015, https://doi.org/10.14406/acu.2016.002