Environmental Engineering Research

  • 제20권2호
  • /
  • Pages.141-148
  • /
  • 2015
  • /
  • 1226-1025(pISSN)
  • /
  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
권호 표지
DOI QR코드

DOI QR Code

Activation and immobilization of phenol-degrading bacteria on oil palm residues for enhancing phenols degradation in treated palm oil mill effluent

  • Tosu, Panida (Environmental Biotechnology Research Unit, Faculty of Environmental Management, Prince of Songkla University) ;
  • Luepromchai, Ekawan (Department of Microbiology, Faculty of Science, Chulalongkorn University) ;
  • Suttinun, Oramas (Environmental Biotechnology Research Unit, Faculty of Environmental Management, Prince of Songkla University)
  • 투고 : 2014.06.24
  • 심사 : 2015.03.03
  • 발행 : 2015.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The presence of phenols in treated palm oil mill effluent (POME) is an environmental concern due to their phytotoxicity and antimicrobial activity. In this study, phenol-degrading bacteria, Methylobacterium sp. NP3 and Acinetobacter sp. PK1 were immobilized on oil palm empty fruit bunches (EFBs) for removal of phenols in the treated POME. The bacterial exopolysaccharides (EPS) were responsible for cell adhesion to the EFBs during the immobilization process. These immobilized bacteria could effectively remove up to 5,000 mg/L phenol in a carbon free mineral medium (CFMM) with a greater degradation efficiency and rate than that with suspended bacteria. To increase the efficiency of the immobilized bacteria, three approaches, namely activation, acclimation, and combined activation and acclimation were applied. The most convenient and efficient strategy was found when the immobilized bacteria were activated in a CFMM containing phenol for 24 h before biotreatment of the treated POME. These activated immobilized bacteria were able to remove about 63.4% of 33 mg/L phenols in the treated POME, while non-activated and/or acclimated immobilized bacteria could degrade only 35.0%. The activated immobilized bacteria could be effectively reused for at least ten application cycles and stored for 4 weeks at $4^{\circ}C$ with the similar activities. In addition, the utilization of the abundant EFBs gives value-added to the palm oil mill wastes and is environmentally friendly thus making it is attractive for practical application.

키워드

참고문헌

  1. Rupani PF, Singh RP, Ibrahim MH, Esa N. Review of current palm oil mill effluent (POME) treatment methods: Vermicomposting as a sustainable practice. World Appl. Sci. J. 2010;10:1190-1201.
  2. Chavalparit O, Rulkens WH, Mol APJ, Khaodhair S. Options for environmental sustainability of the crude palm oil industry in Thailand through enhancement of industrial ecosystems. Environ. Dev. Sustain. 2006;8:271-287. https://doi.org/10.1007/s10668-005-9018-z
  3. Ahmad AL, Ismail S, Bhatia S. Water recycling from palm oil mill effluent (POME) using membrane technology. Desalination 2003;157:87-95. https://doi.org/10.1016/S0011-9164(03)00387-4
  4. Alam MZ, Ameem ES, Muyibi SA, Kabbashi NA. The factors affecting the performance of activated carbon prepared from oil palm empty fruit bunches for adsorption of phenol. Chem. Eng. J. 2009;155:191-198. https://doi.org/10.1016/j.cej.2009.07.033
  5. Cordova-Rosa SM, Dams RI, Cordova-Rosa EV, Radetski MR, CorreaAXR, Radetski CM. Remediation of phenol-contaminated soil by a bacterial consortium and Acinetobacter calcoaceticus isolated from an industrial wastewater treatment plant. J. Hazard. Mater. 2009;164:61-66. https://doi.org/10.1016/j.jhazmat.2008.07.120
  6. Kaewmai R, H-Kittikun A, Suksaroj C, Musikavong C. Alternative technologies for the reduction of greenhouse gas emissions from palm oil mills in Thailand. Environ. Sci. Tech. 2013;47:12417-12425. https://doi.org/10.1021/es4020585
  7. Wu YT, Mohammad WA, Jahim MJ, Anuar N. A holistic approach to managing palm oil mill effluent (POME): Biotechnological advances in the sustainable reuse of POME. Biotechnol. Adv. 2009;27:40-52. https://doi.org/10.1016/j.biotechadv.2008.08.005
  8. Ramos-Cormenzana A, Juarez-Jimenez B, Garcia-Pareja MP. Antimicrobial activity of olive mill wastewaters (alpechin) and biotransformed olive oil mill wastewater. Int. Biodeterior. Biodegradation 1996;38:283-290. https://doi.org/10.1016/S0964-8305(96)00061-3
  9. Kilic NK, Karacakaya P, Duygu E, Donmez G. Biodegradation of phenol by Synechocystis sp. in media including triacontanol hormone. Water Environ. J. 2012; 26:94-99. https://doi.org/10.1111/j.1747-6593.2011.00267.x
  10. Hernandez JE, Edyvean RGJ. Inhibition of biogas production and biodegradability by substituted phenolic compounds in anaerobic sludge. J. Hazard. Mater. 2008;160:20-28. https://doi.org/10.1016/j.jhazmat.2008.02.075
  11. Neoh CH, Lam CY, Lim CK, Yahya A, Ibrahim Z. Decolorization of palm oil mill effluent using growing cultures of Curvularia clavata. Environ. Sci. Pollut. Res. 2014;21:4397-4408. https://doi.org/10.1007/s11356-013-2350-1
  12. Tuck KL, Hayball PJ. Major phenolic compounds in olive oil: metabolism and health effects. J. Nutr. Biochem. 2002;13: 636-644. https://doi.org/10.1016/S0955-2863(02)00229-2
  13. D'Annibale A, Casa R, Pieruccetti F, Ricci M, Marabottini R. Lentinula edodes removes phenols from olive-mill wastewater: impact on durum wheat (Triticum durum Desf.) germinability. Chemosphere 2004;54:887-894. https://doi.org/10.1016/j.chemosphere.2003.10.010
  14. Shirzad-Sibon M, Jafari S-J, Farrokhi M, Yang JK. Removal of Phenol from Aqueous Solutions by Activated Red Mud: Equilibrium and Kinetics Studies. Environ. Eng. Res. 2013;18: 247-252. https://doi.org/10.4491/eer.2013.18.4.247
  15. Said M, Ahmad A, Mohammad AW. Removal of phenol during ultrafiltration of palm oil mill effluent (POME): Effect of pH, ionic strength, pressure and temperature. Der. Pharma. Chemica. 2013;5:190-196.
  16. Di Gioia D, Bertin L, Fava F, Marchetti L. Biodegradation of hydroxylated and methoxylated benzoic, phenylacetic and phenylpropenoic acids present in olive mill wastewaters by two bacterial strains. Res. Microbiol. 2001;152:83-93. https://doi.org/10.1016/S0923-2508(00)01171-2
  17. Di Gioia D, Fava F, Bertin L, Marchetti L. Biodegradation of synthetic and natural occurring mixtures of mono-cyclic aromatic compounds present in olive mill wastewaters by two aerobic bacteria. Appl. Microbiol. Biotechnol. 2001;55:619-626. https://doi.org/10.1007/s002530000554
  18. Piperidou CI, Chaidou CI, Stalikas CD, Soulti K, Pilidis GA, Balis C. Bioremediation of olive oil mill wastewater: chemical alterations induced by Azotobacter vinelandii. J. Agric. Food Chem. 2000;48:1941-1948. https://doi.org/10.1021/jf991060v
  19. Lakhtar H, Ismaili-Alaoui M, Philippoussis A, Perraud-Gaime I, Roussos S. Screening of strains of Lentinula edodes grown on model olive mill wastewater in solid and liquid state culture for polyphenol biodegradation. Int. Biodeterior. Biodeg. 2010;64:167-172. https://doi.org/10.1016/j.ibiod.2009.10.006
  20. Garcia GI, Jimenez Pena PR, Bonilla Venceslada JL, Martin MA, Santos MM, Gomez ER. Removal of phenol compounds from olive mill wastewater using Phanerochaetechrysosporium, Aspergillus niger, Aspergillus terreus and Geotrichum candidum. Process. Biochem. 2000;35:751-758. https://doi.org/10.1016/S0032-9592(99)00135-1
  21. Asses N, Ayed L, Bouallagui H, Sayadi S, Hamdi M. Biodegradation of different molecular-mass polyphenols derived from olive mill wastewaters by Geotrichum candidum. Int. Biodeterior. Biodeg. 2009;63:407-413. https://doi.org/10.1016/j.ibiod.2008.11.005
  22. Ergul FE, Sargin S, Ongen G, Sukan FV. Dephenolisation of olive mill wastewater using adapted Trametes versicolor. Int. Biodeterior. Biodeg. 2009;63:1-6. https://doi.org/10.1016/j.ibiod.2008.01.018
  23. Robles A, Lucas R, de Cienfuegos GA, Galvez A. Biomass production and detoxification of wastewaters from the olive oil industry by strains of Penicillium isolated from wastewater disposal ponds. Bioresour. Technol. 2000;74:217-221. https://doi.org/10.1016/S0960-8524(00)00022-5
  24. Ongen G, Gungor G, Kanberoglu B. Decolorisation and dephenolisation potential of selected Aspergillussection Nigristrains-Aspergillus tubingensis in olive mill wastewater. World J. Microbiol. Biotechnol. 2007;23:519-524. https://doi.org/10.1007/s11274-006-9254-x
  25. AhmadiM, Vahabzadeh F, Bonakdarpour B, Mehranian M, Mofarrah E. Phenolic removal in olive oil mill wastewater using loofah-immobilized Phanerochaete chrysosporium. World J. Microbiol. Biotechnol. 2006;22:119-127. https://doi.org/10.1007/s11274-005-9006-3
  26. Limkhuansuwan V, Chaiprasert P. Decolorization of molasses melanoidins and palm oil mill effluent phenolic compounds by fermentative lactic acid bacteria. J. Environ. Sci. 2010;22: 1209-1217. https://doi.org/10.1016/S1001-0742(09)60240-0
  27. Khongkhaem P, Intasiri A, Luepromchai E. Silica-immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 degrade high concentrations of phenol. Lett. Appl. Microbiol. 2011;52: 448-455. https://doi.org/10.1111/j.1472-765X.2011.03019.x
  28. Kasuga K, Nojiri H, Yamane H, Kodama T, Omori T. Cloning and characterization of the genes involvedin the degradation of dibenzofuran by Terrabacter sp. strain DBF63. J. Ferment. Bioeng. 1997;84:387-399. https://doi.org/10.1016/S0922-338X(97)81997-6
  29. Durmaz B, Sanin FD. Effect of carbon to nitrogen ratio on the composition of microbial extracellular polymers in activated sludge. Water Sci. Technol. 2001;44:221-229.
  30. Sanin SL, Sanin FD, Bryers JD. Effect of starvation on the adhesive properties of xenobiotic degrading bacteria. Process. Biochem. 2003;38:909-914. https://doi.org/10.1016/S0032-9592(02)00173-5
  31. Vandevivere P, Kirchman DL. Attachment stimulate exopolysaccharide synthesis by a bacterium. Appl. Environ. Microbiol. 1993;59:3280-3286.
  32. Pattanasupong A, Nagase H, Sugimoto E, et al. Degradation of Carbendazim and 2-4 dichlorophenoxyacetic acid by immobilized consortium on loofa sponge. J. Biosci. Bioeng. 2004;98:28-33. https://doi.org/10.1016/S1389-1723(04)70238-8
  33. APHA, AWWA, WEF. Standard methods for the examination of water and wastewater. 21th ed. Washington DC: American Public Health Association (APHA); 2005.
  34. Lu Q, Sorial GA. Adsorption of phenolics on activated carbon-impact of pore size and molecular oxygen. Chemosphere 2004;55:671-679. https://doi.org/10.1016/j.chemosphere.2003.11.044
  35. Ng YL, Yan R, Chen XG, et al. Use of activated carbon as a support medium for H2S biofiltration and effect of bacterial immobilization on available pore surface. Appl. Microbiol. Biotechnol. 2004;66:259-265. https://doi.org/10.1007/s00253-004-1673-8
  36. Simova ED, Frengova GI, Beshkova DM. Exopolysaccharides produced by mixed culture of yeast Rhodotorula rubra GED10 and yogurt bacteria (Streptococcus thermophilus 13a+ Lactobacillus bulgaricus 2-11). J. Appl. Microbiol. 2004;97: 512-519. https://doi.org/10.1111/j.1365-2672.2004.02316.x
  37. Rosche B, Li XZ, Hauer B, Schmid A, Buehler K. Microbial biofilms: a concept for industrial catalysis? Trends Biotechnol. 2009;27:636-643. https://doi.org/10.1016/j.tibtech.2009.08.001
  38. Kindzierski WB, Fedorak PM, Gray MR, Hrudey SE. Activated carbon and synthetic resins as support material for mathanogenic phenol-degrading consortia-comparison of phenol-degrading activities. Water Environ. Res. 1995;67:108-117. https://doi.org/10.2175/106143095X131259
  39. Ying W, Ye T, Bin H, Zhao HB, Bi JN, Cai BL. Biodegradation of phenol by free and immobilized Acinetobacter sp. strain PD12. J. Environ. Sci. 2007;19:222-225. https://doi.org/10.1016/S1001-0742(07)60036-9
  40. Ahmad SA, Shamaan NA, Arif NM, Koon GB, Shukor MYA, Syed MA. Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL. World J. Microbiol. Biotechnol. 2012;28:347-352. https://doi.org/10.1007/s11274-011-0826-z
  41. Wang X, Gai Z, Yu B, et al. Degradation of carbazole by microbial cells immobilized in magnetic gellan gum gel beads. Appl. Environ. Microbiol. 2007;73:6421-6428. https://doi.org/10.1128/AEM.01051-07
  42. Gonzalez G, Herrera G, Garcia MT, Pena M. Biodegradation of phenolic industrial wastewater in a fluidized bed bioreactor with immobilized cells of Pseudomonas putida. Bioresour. Technol. 2001;80:137-142. https://doi.org/10.1016/S0960-8524(01)00076-1
  43. Kumar H, Mohanty K. Kinetic modeling of phenol biodegradation by mixed microbial culture in static batch mode. Asian J. Water Environ. Pollut. 2012;9:19-24.
  44. Lu Y, Yan L, Wang Y, Zhou S, Fu J, Zhang J. Biodegradation of phenolic compounds from coking wastewater by immobilized white rot fungus Phanerochaete chrysosporium. J. Hazard. Mater. 2009;165:1091-1097 https://doi.org/10.1016/j.jhazmat.2008.10.091
  45. Diao M, Ouedraogo N, Baba-Moussa L, et al. Biodepollution of wastewater containing phenol compounds from leather industry by plant peroxidases. Biodegradation 2011;22:389-396. https://doi.org/10.1007/s10532-010-9410-8

피인용 문헌

  1. Phenolic compounds removal by grasses and soil bacteria after land application of treated palm oil mill effluent: A pot study vol.24, pp.1, 2018, https://doi.org/10.4491/eer.2018.142
  2. A kinetic study of 4-chlorophenol biodegradation by the novel isolated Bacillus subtilis in batch shake flask vol.25, pp.1, 2015, https://doi.org/10.4491/eer.2018.416
  3. Biodegradation of phenolic compounds present in palm oil mill effluent as single and mixed substrates by Trametes hirsuta AK04 vol.55, pp.8, 2020, https://doi.org/10.1080/10934529.2020.1763092
  4. Removal of Phenol from Oil Mill Effluent Using Activated Carbon Prepared from Kernel Shell in Thailand’s Palm Industry vol.53, pp.11, 2020, https://doi.org/10.1252/jcej.20we052
  5. Synthesis and Characterization of Manganese-Modified Black TiO2 Nanoparticles and Their Performance Evaluation for the Photodegradation of Phenolic Compounds from Wastewater vol.14, pp.23, 2015, https://doi.org/10.3390/ma14237422
  6. Biodegradation of 4-chlorophenol in batch and continuous packed bed reactor by isolated Bacillus subtilis vol.301, pp.None, 2022, https://doi.org/10.1016/j.jenvman.2021.113851