Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos

  • Sun, Qiao (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Wei, Wei (Institute for Interdisciplinary Research, Jianghan University) ;
  • Zhao, Juan (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Song, Jia (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Peng, Fang (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zhang, Shaopeng (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zheng, Yonglian (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Chen, Ping (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University) ;
  • Zhu, Wenjun (College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University)
  • Received : 2015.01.20
  • Accepted : 2015.05.19
  • Published : 2015.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.

Keywords

References

  1. Chen S, Xu J, Liu C, Zhu Y, Nelson DR, Zhou S, et al. 2012. Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nat. Commun. 3: 913. https://doi.org/10.1038/ncomms1923
  2. Covert SF, Kapoor P, Lee M, Briley A, Nairn CJ. 2010. Agrobacterium tumefaciens-mediated transformation of Fusariumcircinatum. Mycol. Res. 105: 259-264.
  3. Dai YC, Yang ZL, Cui BK, Yu CJ, Zhou LW. 2009. Species diversity and utilization of medicinal mushrooms and fungi in China. Int. J. Med. Mushrooms 11: 287-302. https://doi.org/10.1615/IntJMedMushr.v11.i3.80
  4. Esteban CI. 2009. [Medicinal interest of Poria cocos (=Wolfiporia extensa)]. Rev. Iberoam. Micol. 26: 103-107. https://doi.org/10.1016/S1130-1406(09)70019-1
  5. Fei X, Zhao MW, Li YX. 2006. Cloning and sequence analysis of a glyceraldehyde-3-phosphate dehydrogenase gene from Ganoderma lucidum. J. Microbiol. 44: 515-522.
  6. Godio RP, Fouces R, Gudina EJ, Martin JF. 2004. Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium. Curr. Genet. 46: 287-294. https://doi.org/10.1007/s00294-004-0533-5
  7. Kobira S, Atsumi T, Kakiuchi N, Mikage M. 2012. Difference in cultivation characteristics and genetic polymorphism between Chinese and Japaneses trains of Wolfiporia cocos Ryvarden et Gilbertson (Poria cocos Wolf). J. Nat. Med. 66: 493-499. https://doi.org/10.1007/s11418-011-0612-0
  8. Kubo T, Terabayashi S, Takeda S, Sasaki H, Aburada M, Miyamoto K. 2006. Indoor cultivation and cultural characteristics of Wolfiporia cocos sclerotia using mushroom culture bottles. Biol. Pharm. Bull. 29: 1191-1196. https://doi.org/10.1248/bpb.29.1191
  9. Liu L, Zhao D, Zheng L, Hsiang T, Wei Y, Fu Y, Huang J. 2013. Identification of virulence genes in the crucifer anthracnose fungus Colletotrichum higginsianum by insertional mutagenesis. Microb. Pathog. 64: 6-17. https://doi.org/10.1016/j.micpath.2013.06.001
  10. Qin L, Gong X, Xie J, Jiang D, Cheng J, Li G, et al. 2011. Phosphoribosylamidotransferase, the first enzyme for purine de novo synthesis, is required for conidiation in the sclerotial mycoparasite Coniothyrium minitans. Fungal Genet. Biol. 48: 956-965. https://doi.org/10.1016/j.fgb.2011.06.007
  11. Shi L, Fang X, Li MJ, Mu DS, Ren A, Tan Q, et al. 2012. Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum. World J. Microbiol. Biotechnol. 28: 283-291. https://doi.org/10.1007/s11274-011-0818-z
  12. Stoop JMH, Mooibroek H. 1999. Advances in genetic analysis and biotechnology of the cultivated button mushroom, Agaricus bisporus. Appl. Microbiol. Biotechnol. 52: 474-483. https://doi.org/10.1007/s002530051548
  13. Wang KQ, Yin XR, Huang H, Fu J, Feng HG, Wang Q, Sun G. 2012. Production status and industrialization development countermeasures of Poria in Hubei Province. Modern Chin. Med. 14: 24-27.

Cited by

  1. The Kinome of Edible and Medicinal Fungus Wolfiporia cocos vol.7, pp.None, 2015, https://doi.org/10.3389/fmicb.2016.01495
  2. A simple and efficient method for successful gene silencing of HspA1 in Trametes hirsuta AH28-2 vol.110, pp.12, 2017, https://doi.org/10.1007/s10482-017-0904-9
  3. The Phosphatome of Medicinal and Edible Fungus Wolfiporia cocos vol.75, pp.2, 2015, https://doi.org/10.1007/s00284-017-1356-1
  4. Gentic overexpression increases production of hypocrellin A in Shiraia bambusicola S4201 vol.57, pp.2, 2015, https://doi.org/10.1007/s12275-019-8259-8
  5. Biogenesis of macrofungal sclerotia: influencing factors and molecular mechanisms vol.104, pp.10, 2015, https://doi.org/10.1007/s00253-020-10545-8
  6. Functional Analysis of Sterol O-Acyltransferase Involved in the Biosynthetic Pathway of Pachymic Acid in Wolfiporia cocos vol.27, pp.1, 2015, https://doi.org/10.3390/molecules27010143