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Preparation of Egg White Liquid Hydrolysate (ELH) and Its Radical-Scavenging Activity

  • Noh, Dong Ouk (Department of Hotel Culinary Arts and Nutrition, Kaya University) ;
  • Suh, Hyung Joo (Department of Food and Nutrition, Graduate School, Korea University)
  • Received : 2015.06.02
  • Accepted : 2015.07.21
  • Published : 2015.09.30

Abstract

In the present study, an optimum protease was selected to hydrolyze the egg white liquid protein for the antioxidant peptides. Alcalase treatment yielded the highest amount of ${\alpha}$-amino groups (15.27 mg/mL), while the control (no enzymatic hydrolysis) showed the lowest amount of ${\alpha}$-amino groups (1.53 mg/mL). Alcalase also gave the highest degree of hydrolysis (DH) value (43.2%) and was more efficient for egg white liquid hydrolysis than the other enzymes. The Alcalase hydrolysate had the highest radical-scavenging activity (82.5%) at a concentration of 5.0 mg/mL. The conditions for enzymatic hydrolysis of egg white liquid with Alcalase were selected as substrate : water ratio of 2:1. Five percent Alacalse treatment did not show significant (P>0.05) increases of DH and ${\alpha}$-amino nitrogen content after 24 hhydrolysis. Thirty two hour-hydrolysis with 5% Alcalase is sufficient to make antioxidative egg white liquid hydrolysate from egg white liquid. DPPH and ABTS radical-scavenging activities were significantly (P<0.05) higher after enzymatic digestion. These results suggest that active peptides released from egg-white protein are effective radical-scavengers. Thus, this approach may be useful for the preparation of potent antioxidant products.

Keywords

References

  1. Kitts DD, Weiler K. 2003. Bioactive proteins and peptides from food sources. Applications of bioprocesses used in isolation and recovery. Curr Pharm Des 9: 1309-1323. https://doi.org/10.2174/1381612033454883
  2. Murray BA, FitzGerald RJ. 2007. Angiotensin converting enzyme inhibitory peptides derived from food proteins: biochemistry, bioactivity and production. Curr Pharm Des 13: 773-791. https://doi.org/10.2174/138161207780363068
  3. Meisel H. 2004. Multifunctional peptides encrypted in milk proteins. Biofactors 21: 55-61. https://doi.org/10.1002/biof.552210111
  4. Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. J Agric Food Chem 53: 1841-1856. https://doi.org/10.1021/jf030723c
  5. Revilla E, Maria CS, Miramontes E, Bautista J, Garcia-Martínez A, Cremades O, Cert R, Parrado J. 2009. Nutraceutical composition, antioxidant activity and hypocholesterolemic effect of a water-soluble enzymatic extract from rice bran. Food Res Int 42: 387-393. https://doi.org/10.1016/j.foodres.2009.01.010
  6. Megias C, Pedroche J, Yust MM, Giron-Calle J, Alaiz M, Millan F, Vioque J. 2008. Production of copper-chelating peptides after hydrolysis of sunflower proteins with pepsin and pancreatin. LWT−Food Sci Technol 41: 1973-1977.
  7. Xie ZJ, Huang JR, Xu XM, Jin ZY. 2008. Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chem 111: 370-376. https://doi.org/10.1016/j.foodchem.2008.03.078
  8. Li XX, Han LJ, Chen LJ. 2008. In vitro antioxidant activity of protein hydrolysates prepared from corn gluten meal. J Sci Food Agric 88: 1660-1666. https://doi.org/10.1002/jsfa.3264
  9. Sakanaka S, Tachibana Y. 2006. Active oxygen scavenging activity of egg-yolk protein hydrolysates and their effects on lipid oxidation in beef and tuna homogenates. Food Chem 95: 243-249. https://doi.org/10.1016/j.foodchem.2004.11.056
  10. Cho DY, Jo K, Cho SY, Kim JM, Lim K, Suh HJ, Oh S. 2014. Antioxidant effect and functional properties of hydrolysates derived from egg-white protein. Korean J Food Sci An 34: 362-371. https://doi.org/10.5851/kosfa.2014.34.3.362
  11. Kato A, Ibrahim HR, Watanabe H, Honma K, Kobayashi K. 1989. New approach to improve the gelling and surface functional properties of dried egg white by heating in dry state. J Agric Food Chem 37: 433-437. https://doi.org/10.1021/jf00086a036
  12. Matsudomi N, Ishimura Y, Kato A. 1991. Improvement of gelling properties of ovalbumin by heating in dry state. Agric Biol Chem 55: 879-881. https://doi.org/10.1271/bbb1961.55.879
  13. Sakanaka S, Tachibana Y, Ishihara N, Juneja LR. 2004. Antioxidant activity of egg-yolk protein hydrolysates in a linoleic acid oxidation system. Food Chem 86: 99-103. https://doi.org/10.1016/j.foodchem.2003.08.014
  14. Davalos A, Miguel M, Bartolome B, Lopez-Fandino R. 2004. Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67: 1939-1944. https://doi.org/10.4315/0362-028X-67.9.1939
  15. Benjakul S, Morrissey MT. 1997. Protein hydrolysates from pacific whiting solid wastes. J Agric Food Chem 45: 3423-3430. https://doi.org/10.1021/jf970294g
  16. Cheung LM, Cheung PCK, Ooi VEC. 2003. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 81: 249-255. https://doi.org/10.1016/S0308-8146(02)00419-3
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Miguel M, Alonso MJ, Salaices M, Aleixandre A, Lopez- Fandino R. 2007. Antihypertensive, ACE-inhibitory and vasodilator properties of an egg white hydrolysate: Effect of a simulated intestinal digestion. Food Chem 104: 163-168. https://doi.org/10.1016/j.foodchem.2006.11.016
  19. Mine Y. 1995. Recent advances in the understanding of egg white protein functionality. Trends Food Sci Technol 6: 225-232. https://doi.org/10.1016/S0924-2244(00)89083-4
  20. Lechevalier V, Croguennec T, Pezennec S, Guérin-Dubiard C, Pasco M, Nau F. 2005. Evidence for synergy in the denaturation at the air-water interface of ovalbumin, ovotransferrin and lysozyme in ternary mixture. Food Chem 92: 79-87. https://doi.org/10.1016/j.foodchem.2004.07.006
  21. Adler-Nissen J. 1986. Enzymic Hydrolysis of Food Proteins. Elsevier Applied Science Publishers Ltd., London, UK. p 97-122
  22. Wu JP, Ding XL. 2001. Hypotensive and physiological effect of angiotensin converting enzyme inhibitory peptides derived from soy protein on spontaneously hypertensive rats. J Agric Food Chem 49: 501-506. https://doi.org/10.1021/jf000695n
  23. Sarmadi BH, Ismail A. 2010. Antioxidative peptides from food proteins: A review. Peptides 31: 1949-1956. https://doi.org/10.1016/j.peptides.2010.06.020
  24. Pena-Ramos EA, Xiong YL. 2003. Whey and soy protein hydrolysates inhibit lipid oxidation in cooked pork patties. Meat Sci 64: 259-263. https://doi.org/10.1016/S0309-1740(02)00187-0
  25. Pena-Ramos EA, Xiong YL, Arteaga GE. 2004. Fractionation and characterisation for antioxidant activity of hydrolysed whey protein. J Sci Food Agric 84: 1908-1918. https://doi.org/10.1002/jsfa.1886
  26. Kong B, Xiong YL. 2006. Antioxidant activity of zein hydrolysates in a liposome system and the possible mode of action. J Agric Food Chem 54: 6059-6068. https://doi.org/10.1021/jf060632q
  27. Mendis E, Rajapakse N, Kim SK. 2005. Antioxidant properties of a radical-scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. J Agric Food Chem 53: 581-587. https://doi.org/10.1021/jf048877v
  28. Jun SY, Park PJ, Jung WK, Kim SK. 2004. Purification and characterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein. Eur Food Res Technol 219: 20-26. https://doi.org/10.1007/s00217-004-0882-9
  29. Wu HC, Chen HM, Shiau CY. 2003. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 36: 949-957. https://doi.org/10.1016/S0963-9969(03)00104-2
  30. van der Ven C, Gruppen H, de Bont DBA, Voragen AGJ. 2002. Correlations between biochemical characteristics and foam-forming and -stabilizing ability of whey and casein hydrolysates. J Agric Food Chem 50: 2938-2946. https://doi.org/10.1021/jf011190f
  31. Lopez LMI, Brullo A, Natalucci CL, Caffini NO, Sorgentini DA, Wagner JR. 1998. Thermal behavior, solubility and structural properties of soy concentrate hydrolyzed by new plant proteases. J Food Biochem 22: 125-141. https://doi.org/10.1111/j.1745-4514.1998.tb00235.x
  32. Qi M, Hettiarachchy NS, Kalapathy U. 1997. Solubility and emulsifying properties of soy protein isolates modified by pancreatin. J Food Sci 62: 1110-1115. https://doi.org/10.1111/j.1365-2621.1997.tb12224.x
  33. Constantinides A, Adu-Amankwa B. 1980. Enzymatic modification of vegetable protein: Mechanism, kinetics, and production of soluble and partially soluble protein in a batch reactor. Biotechnol Bioeng 22: 1543-1565. https://doi.org/10.1002/bit.260220804
  34. Gibbs BF, Zougman A, Masse R, Mulligan C. 2004. Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Res Int 37: 123-131. https://doi.org/10.1016/j.foodres.2003.09.010
  35. Wu JH, Wang Z, Xu SY. 2008. Enzymatic production of bioactive peptides from sericin recovered from silk industry wastewater. Process Biochem 43: 480-487. https://doi.org/10.1016/j.procbio.2007.11.018
  36. Manso MA, Miguel M, Even J, Hernandez R, Aleixandre A, Lopez-Fandino R. 2008. Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats. Food Chem 109: 361-367. https://doi.org/10.1016/j.foodchem.2007.12.049
  37. Penta-Ramos EA, Xiong YL. 2002. Antioxidant activity of soy protein hydrolysates in a liposomal system. J Food Sci 67: 2952-2956. https://doi.org/10.1111/j.1365-2621.2002.tb08844.x
  38. Chen HM, Muramoto K, Yamauchi F, Fujimoto K, Nokihara K. 1998. Antioxidative properties of histidine-containing peptides designed from peptide fragments found in the digests of a soybean protein. J Agric Food Chem 46: 49-53. https://doi.org/10.1021/jf970649w
  39. Okada Y, Okada M. 1998. Scavenging effect of water soluble proteins in broad beans on free radicals and active oxygen species. J Agric Food Chem 46: 401-406. https://doi.org/10.1021/jf970470l
  40. Hernandez-Ledesma B, Davalos A, Bartolome B, Amigo L. 2005. Preparation of antioxidant enzymatic hydrolysates from ${\alpha}$-lactalbumin and ${\beta}$-lactoglobulin. Identification of active peptides by HPLC-MS/MS. J Agric Food Chem 53: 588-593. https://doi.org/10.1021/jf048626m
  41. Pihlanto A. 2006. Antioxidative peptides derived from milk proteins. Int Dairy J 16: 1306-1314. https://doi.org/10.1016/j.idairyj.2006.06.005

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