한국균학회지 (The Korean Journal of Mycology)

  • 제43권3호
  • /
  • Pages.165-173
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  • 2015
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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국내 아까시나무 숲에서 수집한 감자덩이버섯(Mattirolomyces terfezioides)의 균사 생장 특성과 효소 활성

Characteristics of Mycelial Growth and Enzyme Activities of Mattirolomyces terfezioides Collected from Robinia pseudoacacia Forest in Korea

  • 전성민 (국립산림과학원 화학미생물과) ;
  • 왕은진 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Wang, Eun-Jin (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 투고 : 2015.07.19
  • 심사 : 2015.09.16
  • 발행 : 2015.09.30
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초록

감자덩이버섯(Mattirolomyces terfezioides)은 단맛을 가진 트러플의 한 종류로, 주로 아까시나무(Robinia pseudoacacia)와 외생균근을 형성한다. 상업적으로 인공재배할 만한 가치가 있는 버섯으로, 본 연구에서는 우리나라 아까시나무 숲에서 수집한 감자덩이버섯균의 균사 생장특성과 효소활성에 대해 조사한 바를 처음으로 보고하고자 한다. 이 균은 PDA 배지, $30^{\circ}C$에서 가장 잘 생장하였고, 액체배양 시에는 pH 8.0의 MMN 배지에서 균 생장력이 가장 우수하였다. 또한 암모늄염보다는 질산염 형태의 무기질소원이 함유된 액체배지에서 균체량이 1.8배 더 높았으며, 효소 검색용 고체배지 상에서 carboxymethylcellase와 laccase 활성을 나타냈다.

Mattirolomyces terfezioides is a type of sweet truffles that predominantly form ectomycorrhizae with Robinia pseudoacacia. It is also worthy of artificial cultivation. This is the first report on characteristics of mycelial growth and enzyme activities of M. terfezioides collected from R. pseudoacacia forest in Korea. M. terfezioides showed the highest mycelial growth when cultured on potato dextrose agar (PDA) at $30^{\circ}C$ or in modified Melin-Norkran's liquid medium (pH 8.0). The biomass of M. terfezioides was higher in liquid medium containing nitrate-nitrogen than ammonium-nitrogen by 1.8 fold. The mycelia of M. terfezioides showed both carboxymethylcellulase and laccase activities on solid media for enzyme screening.

키워드

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