Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Influence of insect pollinators on gene transfer from GM to non-GM soybeans

GM 콩의 도입유전자 이동에 미치는 화분 매개충의 영향

  • Lee, Bumkyu (Biosafety Division, National Academy of Agricultural Science) ;
  • Kim, Jun Hyeong (Biosafety Division, National Academy of Agricultural Science) ;
  • Sohn, Soo In (Biosafety Division, National Academy of Agricultural Science) ;
  • Kweon, Soon Jong (Central Area Crop Breeding Division, National Institute of Crop Science) ;
  • Park, Kee Woong (Department of Crop Science, Chungnam National University) ;
  • Chung, Young Soo (Department of Genetic Engineering, Dong-A University) ;
  • Lee, Si Myung (Biosafety Division, National Academy of Agricultural Science)
  • 이범규 (국립농업과학연구원 생물안전성과) ;
  • 김준형 (국립농업과학연구원 생물안전성과) ;
  • 손수인 (국립농업과학연구원 생물안전성과) ;
  • 권순종 (국립식량과학원 중부작물과) ;
  • 박기웅 (충남대학교 식물자원학과) ;
  • 정영수 (동아대학교 유전공학과) ;
  • 이시명 (국립농업과학연구원 생물안전성과)
  • Received : 2015.08.17
  • Accepted : 2015.09.08
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops are also increasing. One of the major concern in risk assessment is the possible development of hybrids through interspecific and intergeneric crosses with related species. This study was conducted to investigate the pollinator have an influence on insect-mediated gene transfer from GM soybeans. Hybrid was induced from GM soybeans by honeybee and western flower thrips, and non-GM soybeans were used as pollen receptor. The analysis for gene-flow was conducted by herbicide selection, immunostrip test, and PCR analysis. In the result of the analysis, three hybrids were detected on the distance 15, 75, 105 cm from pollen source in western flower thrips treatment. In honeybee treatment, one hybrid was detected in the farthest distance (300 cm). These results suggested honeybee and western flower thrips have a possibility they can transfer the introduced gene from GM soybeans to non-GM soybeans.

Keywords

References

  1. Ahmad QN, Britten EJ, Byth BE. 1977. Inversion bridges and meiotic behaviour in species hybrids of soybeans. Journal of Heredity. 68:360-364. https://doi.org/10.1093/oxfordjournals.jhered.a108860
  2. Ahrent DK, Caviness CE. 1994. Natural cross-pollinaion of twelve soybean cultivars in Arkansas. Crop Science. 34: 376-378. https://doi.org/10.2135/cropsci1994.0011183X003400020013x
  3. Al-Ahmad H, Gressel J. 2006. Mitigation using tandem construct containing a selectively unfit gene precludes establishment of Brassica napus transgenes in hybrids and backcrosses with weedy Brassica rapa. Plant Biotech 4(1): 23-33. https://doi.org/10.1111/j.1467-7652.2005.00153.x
  4. Baek HJ, Sohn SI, Cho MR, Lee GS, Oh YJ, Park JS, Lee KJ, Oh SD, Suh SC, Ryu TH. 2010, Development of protocol for analyzing pollinator insect-mediated gene transfer from gm crop. Korean Journal of International Agriculture. 22(3): 293-297.
  5. Biosafety white paper. 2013. LMO perception and communication (http://www.biosafety.or.kr).
  6. Cardinala S. Strakab J, Danfortha BN. 2010. Comprehensive phylogeny of apid bees reveals the evolutionary origins and antiquity of cleptoparasitism. PNAS. 107(37):16207-16211. https://doi.org/10.1073/pnas.1006299107
  7. Chiang YC, Kiang YT. 1987. Geometric position of genotypes, honeybee foraging patterns and outcrossing in soybean. Botanical Bulletin of Academia Sinica. 28:1-11.
  8. Danforth BN, Sipes S, Fang J, Brady SG. 2006. The history of early bee diversification based on five genes plus morphology. PNAS. 103(41):15118-15123. https://doi.org/10.1073/pnas.0604033103
  9. Huang KS, Lee SB, Yeh Y, Shen GS, Mei E, Chang CM. 2010. Taqman real-time quantitative PCR for identification of western flower thrip (Frankliniella occidentalis) for plant quarantine. Biology letters. 6:555-557. https://doi.org/10.1098/rsbl.2009.1060
  10. Jamse C. 2015. Global status of commercialized biotech/GM crops: 2014, BRIEF 49 (http://www.isaaa.org).
  11. Jang SJ, Park SJ, Piao XM, Song HL, Hwang TY, Cho YG, Liu XH, Woo SH, Kang JH, Kim HS. 2010. Genetic diversity and relationships of Korean, Japanese and Chinese Jilin provincial wild soybeans (Glycine soja Sieb. And Zucc.) based on SSR markers. Korean Journal Breeding Science. 42(1):87-99.
  12. Karasawa K. 1952. Crossing exeriments with Glycine soja and G. gracilis. Genetica 26:57-358
  13. KBCH. 2015. Stats of GM crops in Korea (http://www. biosafety.or.kr).
  14. Nakayama Y, Yamaguchi H. 2002, Natural hybridization in wild soybean (Glycine max ssp. soja) by pollen flow from cultivated soybean (Glycine max ssp. max) in a designed population. Weed Biology and Management. 2:25-30. https://doi.org/10.1046/j.1445-6664.2002.00043.x
  15. OECD. 2000. Consensus document on the biology of Glycine max (L.) Merr.(soybean).
  16. Snow AA and Moran-Palma P. 1997. Commercialization of transgenic plants: potential ecological risks. BioScience. 47: 86-96. https://doi.org/10.2307/1313019
  17. Warwick SI, Beckie HJ, Hall LM. 2009. Gene flow, invasiveness, and ecological impact of genetically modified crops. The Year in Evolutionary Biology 2009: Ann. N.Y. Acad. Sci. 1168:72-99.

Cited by

  1. A study on the establishment of isolation distances for environmental release of biotech crops vol.44, pp.2, 2017, https://doi.org/10.7744/kjoas.20170018
  2. Comparison of the Nutritional Compositions of hybrid Soybean between β-carotene Enhanced Transgenic Soybean and wild Soybean vol.32, pp.4, 2015, https://doi.org/10.12719/ksia.2020.32.4.339
  3. Pollen-mediated exclusive gene flow from transgenic crops vol.67, pp.3, 2021, https://doi.org/10.1080/09670874.2020.1748747