농업과학연구 (Korean Journal of Agricultural Science)

  • 제42권3호
  • /
  • Pages.167-175
  • /
  • 2015
  • /
  • 2466-2402(pISSN)
  • /
  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
권호 표지
DOI QR코드

DOI QR Code

수분스트레스에 의한 케일 내 글루코시놀레이트 변화

Variations of glucosinolates in kale leaves (Brassica oleracea var. acephala) treated with drought-stress in autumn and spring seasons

  • 정나래 (충남대학교 생물환경화학과) ;
  • 천진혁 (충남대학교 생물환경화학과) ;
  • 박은재 (충남대학교 생물환경화학과) ;
  • 임예훈 (충남대학교 생물환경화학과) ;
  • 김선주 (충남대학교 생물환경화학과)
  • Jeong, Na-Rae (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Chun, Jin-Hyuk (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Park, Eun-Jae (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Lim, Ye-Hoon (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Kim, Sun-Ju (Department of Bio-Environmental Chemistry, Chungnam National University)
  • 투고 : 2015.05.08
  • 심사 : 2015.08.19
  • 발행 : 2015.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study aimed to investigate the effects of drought stress on the accumulation of glucosinolates (GSLs) in the leaves of Kale cultivated in autumn and spring. HPLC analysis guided to identify seven GSLs including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrasscin. Quantification of GSLs revealed that the contents of sigirin was the highest (45%) followed by the level of progoitrin (24%) in terms of total GSLs. The ranges of total GSL contents was 1.16 (84)-15.88 (89 DAS, ${\mu}mol/g$ dry wt. (DW)) in treatment plot and 1.23 (84)-7.05 (74 DAS, ${\mu}mol/g$ dry wt.) in control plot showed the enhancement in the contents of GSLs in treatment than in the control plot. The present results evidenced that the variation of total GSL contents were depending on the harvest period. In 105 DAS, comparatively no differences in the GSL contents on each sample in autumn season, whereas in spring season, although there was decrease in the GSLs tendency from 74 DAS to 84 DAS in both control and treatment plot, the GSL contents of treatment plot was dramatically increased in 89 DAS. In treatment plot, the GSL contents on 89 DAS (1.16) was 15 fold higher to 84 DAS ($15.88{\mu}mol/g$ DW). The variation in the contents of GSL in spring and autumn did not documented significant differences because of their differences in the growth time and cultivation conditions. In conclusion, the GSL contents in kale was likely to be affected by drought stress treatment. Scrutiny and further research for exact relation between drought stress and GSL contents in kale should be needed.

키워드

참고문헌

  1. Brown AF, Yousef GG, Jeffery EH, Klein BP, Wallig MA, Kushad MM, Juvik JA. 2002. Glucosinolate profiles in broccoli: Variation in levels and implications in breeding for cancer chemoprotection. J. Amer. Soc. Hort. Sci. 127: 807-813.
  2. Clarke DB. 2010. Glucosinolates, structures and analysis in food. Anal. Methods 2:310-325. https://doi.org/10.1039/b9ay00280d
  3. Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085-1097. https://doi.org/10.1105/tpc.7.7.1085
  4. Fahey JW, Zalcmann AT, Talalay P. 2001. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5-51. https://doi.org/10.1016/S0031-9422(00)00316-2
  5. Halkier BA, Gershenzon J. 2006. Biology and biochemistry of glucosinolates. Annu. Rev. Plant Biol. 57:303-333. https://doi.org/10.1146/annurev.arplant.57.032905.105228
  6. Holst B, Williamson G. 2004. A critical review of the bioavailability of glucosinolates and related compounds. Nat. Prod. Rep. 21:425-447. https://doi.org/10.1039/b204039p
  7. Hwang ES, Hong EY, Kim GH. 2012. Determination of bioactive compounds and anti-biocancer effect from extracts of Korean cabbage and cabbage. Korean J. Food & Nutr. 25:259-265. https://doi.org/10.9799/ksfan.2012.25.2.259
  8. Jahangir M, Kim HK, Choi YH, Verpoorte R. 2009. Health-affecting compounds in Brassicaceae. Comprehensive Rev. Food Sci. Food Safety 8:31-43. https://doi.org/10.1111/j.1541-4337.2008.00065.x
  9. Keum YS, Jeong WS, Kong ANT. 2004. Chemoprevention by isothiocyanates and their underlying molecular signaling mechanisms. Mutat. Res. Fundam. Mol. Mech. Mutagen. 555:191-202. https://doi.org/10.1016/j.mrfmmm.2004.05.024
  10. Kim SJ, Kawaharada C, Jin S, Hashimoto M, Ishii G, Yamauchi H. 2007. Structural elucidation of 4-(cystein-S-yl) butyl glucosinolate from the leaves of Eruca sativa. Biosci. Biotechnol. Biochem. 71:114-121. https://doi.org/10.1271/bbb.60400
  11. Lee SM, Rhee SH, Park KY. 1997. Antimutagenic effect of various Cruciferous vegetables in salmonella assaying system. J. Food Hyg. Safety. 12:321-327.
  12. Park KW, Ryu KO. 1998. Functional property and health stuffed vegetables. pp. 172-175. Herbworld, Seoul, Korea.
  13. Poiroux-Gonord F, Bidel LP, Fanciullino AL, Gautier H, Lauri-Lopez F, Urban L. 2010. Health benefits of vitamins and secondary metabolites of fruits and vegetables and prospects to increase their concentrations by agronomic approaches. J. Agric. Food Chem. 58:12065-12082. https://doi.org/10.1021/jf1037745
  14. Rajashekar CB, Carey EE, Zhao X, Oh MM. 2009. Health-promoting phytochemicals in fruits and vegetables: Impact of abiotic stresses and crop production practices. Functional Plant Sci. Biotechnol. 3:30-38.
  15. Rosa E, Heaney RK. 1996. Seasonal variation in protein, mineral and glucosinolate composition of Portuguese cabbages and kale. Animal Feed Sci. Technol. 57:111-127. https://doi.org/10.1016/0377-8401(95)00841-1
  16. Schreiner M. 2005. Vegetable crop management strategies to increase the quantity of phytochemicals. European J. Nutr. 44: 85-94. https://doi.org/10.1007/s00394-004-0498-7
  17. Schmidt S, Zietz M, Schreiner M, Rohn S, Kroh LW, Krumbein A. 2010. Genotypic and climatic influences on the concentration and composition of flavonoids in kale (Brassica oleracea var. sabellica). Food Chem. 119:1293-1299. https://doi.org/10.1016/j.foodchem.2009.09.004
  18. Sun B, Liu N, Zhao Y, Yan H, Wang Q. 2011. Variation of glucosinolates in three edible parts of Chinese kale (Brassica alboglabra Bailey) varieties. Food Chemistry 124(3):941-947. https://doi.org/10.1016/j.foodchem.2010.07.031
  19. Van Etten CH, Daxenbichler ME, Wolff IA. 1969. Natural glucosinolates (thioglucosides) in foods and feeds. J. Agric. Food Chem. 17:483-491. https://doi.org/10.1021/jf60163a013
  20. Wattenberg LW. 1977. Inhibition of carcinogenic effects of polycyclic hydrocarbons by benzylisothicyanate and related compounds. J. Natl. Cancer. Inst. 58:398-398.
  21. Zhang Y, Talalay P. 1994. Anticarcinogenic activities of organic isothiocyanates: Chemistry and mechanisms. Cancer Research 54:1976-1981.
  22. Zhang Y, Wade K., Prestera T, Talalay P. 1996. Quantitative Determination of Isothiocyanates, Dithiocarbamates, Carbon Disulfide, and Related Thiocarbonyl Compounds by Cyclocondensation with 1,2-Benzenedithiol. Analytical Biochemistry 239:160-167. https://doi.org/10.1006/abio.1996.0311

피인용 문헌

  1. Optimizing growth conditions for glucosinolate production in Chinese cabbage vol.59, pp.5, 2018, https://doi.org/10.1007/s13580-018-0084-1
  2. 간척지 토양에서 양액의 전기전도도가 비트 및 순무의 생장에 미치는 영향 vol.37, pp.3, 2018, https://doi.org/10.5338/kjea.2018.37.3.25