Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Effects of CNCPS fraction-enriched proteins on ruminal fermentation and plasma metabolites in holstein steers fed TMR containing low protein

저단백질 TMR을 기초사료로 급여한 홀스타인 거세우에 있어서 CNCPS fraction별 고함유 단백질 공급이 반추위 발효패턴 및 혈액대사물질에 미치는 영향

  • Received : 2015.07.17
  • Accepted : 2015.08.25
  • Published : 2015.09.30
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Abstract

Four ruminally cannulated Holstein steers (BW $401.0{\pm}2.22kg$) fed TMR containing low protein (CP 9.63 %) as a basal diet were used to investigate the effects of cornell net carbohydrates and protein system (CNCPS) fraction enriched protein feeds on rumen fermentation and blood metabolites. The steers used in a $4{\times}4$ Latin square design consumed TMR only (control), TMR with rapeseed meal (AB1), TMR with soybean meal (B2) and TMR with perilla meal (B3C), respectively. The protein feeds were substituted for 30 % crude protein of TMR intake. For measuring ruminal pH, ammonia-N and volatile fatty acids (VFA), ruminal digesta was sampled through ruminal cannula at 1 h-interval after the afternoon feeding. Blood was sampled via the jugular vein after the ruminal digesta sampling. Different CNCPS fraction-enriched proteins did not affect (p>0.05) ruminal pH except B3C being numerically low compared with the other groups. Ammonia-N and VFA were not significantly different among the experimental groups. Numerically low ammonia-N appeared in the steers fed rapeseed meal even though it contained high soluble N composition (A and B1 fractions). The discrepancy is unclear; however this may be related to low protein level in the diet and/or low DM intake. Blood metabolites were not significantly affected by the protein substitution except for blood urea nitrogen that was significantly (p<0.05) increased.

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