Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Photosynthesis rates, growth, and ginsenoside contents of 2-yr-old Panax ginseng grown at different light transmission rates in a greenhouse

  • Jang, In-Bae (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Yu, Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Hong-Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Mo, Hwang-Sung (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Kee-Choon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Hyun, Dong-Yun (Planning and Coordination Division, NIHHS, RDA) ;
  • Lee, Eung-Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee-Hong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, Kyung Hee University)
  • Received : 2014.12.04
  • Accepted : 2015.03.17
  • Published : 2015.10.15
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Abstract

Background: Ginseng is a semishade perennial plant cultivated in sloping, sun-shaded areas in Korea. Recently, owing to air-environmental stress and various fungal diseases, greenhouse cultivation has been suggested as an alternative. However, the optimal light transmission rate (LTR) in the greenhouse has not been established. Methods: The effect of LTR on photosynthesis rate, growth, and ginsenoside content of ginseng was examined by growing ginseng at the greenhouse under 6%, 9%, 13%, and 17% of LTR. Results: The light-saturated net photosynthesis rate ($A_{sat}$) and stomatal conductance ($g_{s}$) of ginseng increased until the LTR reached 17% in the early stage of growth, whereas they dropped sharply owing to excessive leaf chlorosis at 17% LTR during the hottest summer period in August. Overall, 6-17% of LTR had no effect on the aerial part of plant length or diameter, whereas 17% and 13% of LRT induced the largest leaf area and the highest root weight, respectively. The total ginsenoside content of the ginseng leaves increased as the LTR increased, and the overall content of protopanaxatriol line ginsenosides was higher than that of protopanaxadiol line ginsenosides. The ginsenoside content of the ginseng roots also increased as the LTR increased, and the total ginsenoside content of ginseng grown at 17% LTR increased by 49.7% and 68.3% more than the ginseng grown at 6% LTR in August and final harvest, respectively. Conclusion: These results indicate that 13-17% of LTR should be recommended for greenhouse cultivation of ginseng.

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References

  1. Cheon SK, Mok SK, Lee SS, Shin DY. Effects of light intensity and quality on the growth and quality of Korean ginseng (Panax ginseng C.A. Meyer): I. Effects of light intensity on the growth and yield of ginseng plants. Kor J Ginseng Sci 1991;15:21-30.
  2. Proctor JTA. Source-sink relations in North American ginseng seedlings as influenced by leaflet removal. J Ginseng Res 2008;32:337-40. https://doi.org/10.5142/JGR.2008.32.4.337
  3. Jochum GM, Mudge KW, Thomas RB. Elevated temperatures increase leaf senescence and root secondary metabolite concentrations in the understory herb Panax quinquefolius (Araliaceae). Am J Bot 2007;94:819-26. https://doi.org/10.3732/ajb.94.5.819
  4. Park YH, Lee SG, Ahn DJ, Kwon TR, Park SU, Lim HS, Bae H. Diversity of fungal endophytes in various tissues of Panax ginseng Meyer cultivated in Korea. J Ginseng Res 2012;36:211-7. https://doi.org/10.5142/jgr.2012.36.2.211
  5. Kim YC, Lee JH, Bae YS, Sohn BK, Park SK. Development of effective environmentally-friendly approaches to control Alternaria blight and anthracnose diseases of Korean ginseng. Eur J Plant Pathol 2010;127:443-50. https://doi.org/10.1007/s10658-010-9610-4
  6. Lee SS, Kim JM, Cheon SK, Kim YT. Relationship between environmental conditions and the growth of ginseng plant in field: II. Light intensity under shading material and photosynthesis. Kor J Crop Sci 1982;27:169-74.
  7. Lee JS, Lee JH, Ahn IO. Characteristics of resistant lines to high-temperature injury in ginseng (Panax ginseng C.A. Meyer). J Ginseng Res 2010;34:274-81. https://doi.org/10.5142/jgr.2010.34.4.274
  8. Kim DW, Kim JY, You DH, Kim CS, Kim HJ, Park JS, Kim JM, Choi DC, Oh NK. Effect of cultivation using plastic-film house on yield and quality of ginseng in paddy field. Kor J Med Crop Sci 2014;22:210-6. https://doi.org/10.7783/KJMCS.2014.22.3.210
  9. Kwon SG, Lee CY, Oh DJ, Li GY, Cha SW, Lee SW. Changes of growth characteristics and ginsenoside content by growth stages and different planting position in Panax ginseng C.A. Meyer. Kor J Med Crop Sci 2010;18:51-5.
  10. Hyun DY, Hwang JK, Choi SY, Jo JS. Photosynthetic characteristics of Panax ginseng C.A. Meyer: I. Photosynthetic response to changes of light intensity and leaf temperature. Kor J Ginseng Sci 1993;17:240-5.
  11. Lee SS. Characteristics of photosynthesis among new cultivars of ginseng (Panax ginseng C.A. Meyer). J Ginseng Res 2002;26:85-8. https://doi.org/10.5142/JGR.2002.26.2.085
  12. Lee JS, Lee DY, Lee JH, Ahn IO, In JG. Photosynthetic characteristics of resistance and susceptible lines to high temperature injury in Panax ginseng Meyer. J Ginseng Res 2012;36:461-8. https://doi.org/10.5142/jgr.2012.36.4.461
  13. Markwell J, Osterman JC, Mitchell JL. Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynth Res 1995;46:467-72. https://doi.org/10.1007/BF00032301
  14. Oh DJ, Lee CY, Kim SM, Li GY, Lee SJ, Hwang DY, Son HJ, Won JY. Effects of chlorophyll fluorescence and photosynthesis characteristics by planting positions and growth stage in Panax ginseng C.A. Meyer. Kor J Med Crop Sci 2010;18:65-9.
  15. Anderson JM. Photo-regulation of the composition, function, and structure of thylakoid membranes. Plant Physiol 1986;37:93-136. https://doi.org/10.1146/annurev.pp.37.060186.000521
  16. Terashima I. Anatomy of non-uniform leaf photosynthesis. Photosynth Res 1992;31:195-212. https://doi.org/10.1007/BF00035537
  17. Tyystjarvi E, Koivuniemi A, Kettnen R, Aro EM. Small light-harvesting antenna does not protect from photo-inhibition. Plant Physiol 1991;97:477-83. https://doi.org/10.1104/pp.97.2.477
  18. Lee CH. Effect of light intensity and temperature on the photosynthesis and respiration of Panax spp. Kor J Ginseng Sci 1988;12:11-29.
  19. Cho JW, Park HW, Kim MJ, Kim HH, Choi JE. Photosynthetic, morphological and growing characteristics by shading materials in Panax ginseng C.A. Meyer. Kor J Crop Sci 2008;53:256-60.
  20. Yang DC, Yoo HS, Yoon JJ. Investigation on the photo-oxidation of pigment in leaf-burning disease of Panax ginseng: II. Investigation and analysis of physiological reaction mechanism on the chlorophyll bleaching phenomenon. Kor J Ginseng Sci 1987;11:101-10.
  21. Osmond CB. Whatis photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR, Bowyer JR, editors. Photoinhibition of photosynthesis: from molecular mechanisms to the field. Oxford: BIOS Scientific; 1994.
  22. Ort DR, Baker NR. Consideration of photosynthetic efficiency at low light as a major determinant of crop photosynthetic performance. Plant Physiol Biochem 1988;26:555-65.
  23. Yang DC, Chae Q, Lee SJ, Kim YH, Kang YH. Effects of light and photosynthetic electron transport system on the generation of singlet oxygen ($^1O_2$) in ginseng thylakoid membrane. Kor J Ginseng Sci 1990;14:57-62.
  24. Powles SB. Photoinhibition of photosynthesis induced by visible light. Annu Rev Plant Biol 1984;35:15-44. https://doi.org/10.1146/annurev.pp.35.060184.000311
  25. Long SP, Humphries S, Falkowski PG. Photoinhibition of photo-synthesis in nature. Annu Rev Plant Physiol Plant Mol Biol 1994;45:633-62. https://doi.org/10.1146/annurev.pp.45.060194.003221
  26. Proctor JTA, Palmer JW, Follett JM. Growth, dry matter partitioning and photosynthesis in North American ginseng seedlings. J Ginseng Res 2010;34:175-82. https://doi.org/10.5142/jgr.2010.34.3.175
  27. Kim JM, Lee SS, Cheon SR, Cheon SK. Relationship between environmental conditions and the growth of ginseng plant in field: I. Productive structures as affected by planting positions and ages. Kor J Crop Sci 1982;27:94-8.
  28. Lichtenthaler HK, Buschmann C, Doll M, Fietz HJ, Bach T, Kozel U, Meier D, Rahmsdorf U. Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high-light and low-light plants and of sun and shade leaves. Photosynth Res 1981;2:115-41. https://doi.org/10.1007/BF00028752
  29. Jo DK, Yun CY, Kim KD, Kang YH. Estimation of solar radiation distribution in Korea using a satellite. Kor J Sol Energy 2011;31:99-106.
  30. Christensen LP. Ginsenosides chemistry, biosynthesis, analysis, and potential health effects. Adv Food Nutr Res 2009;55:1-99.
  31. Fournier AR, Proctor JT, Gauthier L, Khanizadeh S, Belanger A, Gosselin A, Dorais M. Understory light and root ginsenosides in forest-grown Panax quinquefolius. Phytochemistry 2003;63:777-82. https://doi.org/10.1016/S0031-9422(03)00346-7
  32. Lee MK, Park H, Lee CH. Effect of growth conditions on saponins content and ginsenoside pattern of Panax ginseng. Kor J Ginseng Sci 1987;11:233-51.
  33. Li TJ, Lian ML, Yu D, Shao CH, Piao XC. Effects of several factors on cell growth and ginsenoside accumulation of Panax ginseng suspension culture. Zhongguo Zhong Yao Za Zhi 2013;38:4047-51.
  34. Lee JC, Choi JH, Cheon SK, Lee CH, Jo JS. Studies on the optimum light intensity for growth of Panax ginseng: II. Effect of light intensity on the contents of saponins and free sugar in the ginseng leaf. Kor J Ginseng Sci 1983;28:497-503.
  35. Kim OT, Bang KH, Kim YC, Hyun DY, Kim MY, Cha SW. Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tiss Organ Cult 2009;98:25-33. https://doi.org/10.1007/s11240-009-9535-9
  36. Choi DW, Jung JD, Ha YI, Park HW, In DS, Chung HJ, Liu JR. Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites. Plant Cell Rep 2005;23:557-66. https://doi.org/10.1007/s00299-004-0845-4

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